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Article: The role of jak2a in zebrafish hematopoiesis

TitleThe role of jak2a in zebrafish hematopoiesis
Authors
Issue Date2007
PublisherAmerican Society of Hematology. The Journal's web site is located at http://bloodjournal.hematologylibrary.org/
Citation
Blood, 2007, v. 110 n. 6, p. 1824-1830 How to Cite?
AbstractJanus kinase 2 (Jak2) transduces signals from hematopoietic cytokines, and a gain-of-function mutation (Jak2617V>F) is associated with myeloproliferative diseases, particularly polycythemia vera. In this study, we examined the role of jak2a in zebrafish embryos in knock-down and overexpression studies using morpholinos (MOs) targeting the 5′ untranslated region (UTR) (jak2aUTR-MO) and splicesite junction (jak2aSS-MO) of jak2a, a Jak inhibitor AG490 and a constitutive-active form of jak2a (jak2aca). At 18 and 24 hours after fertilization (hpf), jak2a is expressed predominantly in the intermediate cell mass (ICM; site of primitive hematopoiesis) of wild-type and chordin morphant embryos (characterized by expansion of ICM). Both jak2a MOs and AG490 reduced gata1+ (erythroid) cells in Tg(gata1:GFP) embryos, signal transducer and activation of transcription 5 (stat5) phosphorylation, and gene expression associated with early progenitors (scl and lmo2) and erythroid (gata1, αhe1 and βhe1) and myeloid (spi1 [early] and mpo [late]) lineages. The chordin morphant is associated with increased stat5 phosphorylation, and both jak2a MOs and treatment with AG490 significantly ameliorated ICM expansion and hematopoietic gene upregulation in these embryos. Injection of plasmid encoding jak2a ca significantly increased erythropoiesis and expression of gata1, αhe1 and βhe1, spi1, mpo, and l-plastin. In conclusion, zebrafish jak2a is involved in primitive hematopoiesis under normal and deregulated conditions. © 2007 by The American Society of Hematology.
Persistent Identifierhttp://hdl.handle.net/10722/77404
ISSN
2015 Impact Factor: 11.841
2015 SCImago Journal Rankings: 6.395
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorMa, ACHen_HK
dc.contributor.authorWard, ACen_HK
dc.contributor.authorLiang, Ren_HK
dc.contributor.authorLeung, AYHen_HK
dc.date.accessioned2010-09-06T07:31:32Z-
dc.date.available2010-09-06T07:31:32Z-
dc.date.issued2007en_HK
dc.identifier.citationBlood, 2007, v. 110 n. 6, p. 1824-1830en_HK
dc.identifier.issn0006-4971en_HK
dc.identifier.urihttp://hdl.handle.net/10722/77404-
dc.description.abstractJanus kinase 2 (Jak2) transduces signals from hematopoietic cytokines, and a gain-of-function mutation (Jak2617V>F) is associated with myeloproliferative diseases, particularly polycythemia vera. In this study, we examined the role of jak2a in zebrafish embryos in knock-down and overexpression studies using morpholinos (MOs) targeting the 5′ untranslated region (UTR) (jak2aUTR-MO) and splicesite junction (jak2aSS-MO) of jak2a, a Jak inhibitor AG490 and a constitutive-active form of jak2a (jak2aca). At 18 and 24 hours after fertilization (hpf), jak2a is expressed predominantly in the intermediate cell mass (ICM; site of primitive hematopoiesis) of wild-type and chordin morphant embryos (characterized by expansion of ICM). Both jak2a MOs and AG490 reduced gata1+ (erythroid) cells in Tg(gata1:GFP) embryos, signal transducer and activation of transcription 5 (stat5) phosphorylation, and gene expression associated with early progenitors (scl and lmo2) and erythroid (gata1, αhe1 and βhe1) and myeloid (spi1 [early] and mpo [late]) lineages. The chordin morphant is associated with increased stat5 phosphorylation, and both jak2a MOs and treatment with AG490 significantly ameliorated ICM expansion and hematopoietic gene upregulation in these embryos. Injection of plasmid encoding jak2a ca significantly increased erythropoiesis and expression of gata1, αhe1 and βhe1, spi1, mpo, and l-plastin. In conclusion, zebrafish jak2a is involved in primitive hematopoiesis under normal and deregulated conditions. © 2007 by The American Society of Hematology.en_HK
dc.languageengen_HK
dc.publisherAmerican Society of Hematology. The Journal's web site is located at http://bloodjournal.hematologylibrary.org/en_HK
dc.relation.ispartofBlooden_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshAnimals, Genetically Modifieden_HK
dc.subject.meshCell Divisionen_HK
dc.subject.meshCell Lineageen_HK
dc.subject.meshEmbryo, Nonmammalian - physiologyen_HK
dc.subject.meshEnzyme Activationen_HK
dc.subject.meshErythroid Precursor Cells - metabolismen_HK
dc.subject.meshGene Expression Profilingen_HK
dc.subject.meshGene Expression Regulation, Developmentalen_HK
dc.subject.meshGlycoproteins - metabolismen_HK
dc.subject.meshHematopoiesisen_HK
dc.subject.meshIntercellular Signaling Peptides and Proteins - metabolismen_HK
dc.subject.meshJanus Kinase 2 - genetics - physiologyen_HK
dc.subject.meshNeovascularization, Physiologicen_HK
dc.subject.meshOligonucleotide Array Sequence Analysisen_HK
dc.subject.meshPhosphorylationen_HK
dc.subject.meshSTAT5 Transcription Factor - metabolismen_HK
dc.subject.meshSignal Transductionen_HK
dc.subject.meshZebrafish - embryology - geneticsen_HK
dc.subject.meshZebrafish Proteins - genetics - metabolismen_HK
dc.titleThe role of jak2a in zebrafish hematopoiesisen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0006-4971&volume=110&spage=1824&epage=1830&date=2007&atitle=The+role+of+jak2a+in+zebrafish+hematopoiesisen_HK
dc.identifier.emailLiang, R:rliang@hku.hken_HK
dc.identifier.emailLeung, AYH:ayhleung@hku.hken_HK
dc.identifier.authorityLiang, R=rp00345en_HK
dc.identifier.authorityLeung, AYH=rp00265en_HK
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1182/blood-2007-03-078287en_HK
dc.identifier.pmid17545503-
dc.identifier.scopuseid_2-s2.0-34548822125en_HK
dc.identifier.hkuros147252en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-34548822125&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume110en_HK
dc.identifier.issue6en_HK
dc.identifier.spage1824en_HK
dc.identifier.epage1830en_HK
dc.identifier.eissn1528-0020-
dc.identifier.isiWOS:000249671700023-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridMa, ACH=15849157500en_HK
dc.identifier.scopusauthoridWard, AC=7402090618en_HK
dc.identifier.scopusauthoridLiang, R=26643224900en_HK
dc.identifier.scopusauthoridLeung, AYH=7403012668en_HK
dc.identifier.citeulike10830166-

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