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Article: Plasma apolipoprotein E concentration is an important determinant of phospholipid transfer protein activity in type 2 diabetes mellitus

TitlePlasma apolipoprotein E concentration is an important determinant of phospholipid transfer protein activity in type 2 diabetes mellitus
Authors
Issue Date2006
PublisherJohn Wiley & Sons Ltd. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/10009394
Citation
Diabetes/Metabolism Research And Reviews, 2006, v. 22 n. 4, p. 307-312 How to Cite?
AbstractBackground: Phospholipid transfer protein (PLTP) transfers phospholipids between lipoproteins and plays an important role in HDL metabolism. PLTP exists as a high-activity and a low-activity form in the circulation. In vitro studies have shown that apolipoprotein (apo) E is involved in maintaining PLTP in the active form, while the low-activity form is associated with apo AI. We have therefore investigated whether plasma apo AI, B and E concentrations are important determinants of plasma PLTP activity in type 2 diabetes, a condition associated with increased plasma PLTP activity. Methods: Plasma PLTP activity was assayed by measuring the transfer of radiolabelled phosphatidylcholine from liposomes to HDL; apo AI and B by rate nephelometry and apo E by a 2-point turbidimetric assay. Results: Type 2 diabetic patients (n = 230) had higher PLTP activity than controls (n = 97) (2374 ± 628 nmol/mL/h versus 1862 ± 585 respectively, p < 0.01). They also had increased fasting triglyceride and low HDL. Plasma apo B (p < 0.01) and apo E (p < 0.05) were increased, whereas apo AI was reduced (p < 0.01). Univariate analysis showed that plasma PLTP activity correlated mainly with apolipoproteins AI and E. Stepwise regression analysis showed that apo E was the main determinant of plasma PLTP activity, accounting for 23% of its variability in the diabetic subjects and 8% in the controls respectively. Conclusions: The associations between plasma apo AI and E concentrations and PLTP activity suggest that these apolipoproteins are important regulators of PLTP activity in vivo. The increase in PUP activity in type 2 diabetes is partly related to the changes in these apolipoproteins. Copyright © 2006 John Wiley & Sons, Ltd.
Persistent Identifierhttp://hdl.handle.net/10722/77362
ISSN
2012 Impact Factor: 2.968
2015 SCImago Journal Rankings: 1.617
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorTan, KCBen_HK
dc.contributor.authorShiu, SWMen_HK
dc.contributor.authorWong, Yen_HK
dc.contributor.authorWong, WKen_HK
dc.contributor.authorTam, Sen_HK
dc.date.accessioned2010-09-06T07:31:05Z-
dc.date.available2010-09-06T07:31:05Z-
dc.date.issued2006en_HK
dc.identifier.citationDiabetes/Metabolism Research And Reviews, 2006, v. 22 n. 4, p. 307-312en_HK
dc.identifier.issn1520-7552en_HK
dc.identifier.urihttp://hdl.handle.net/10722/77362-
dc.description.abstractBackground: Phospholipid transfer protein (PLTP) transfers phospholipids between lipoproteins and plays an important role in HDL metabolism. PLTP exists as a high-activity and a low-activity form in the circulation. In vitro studies have shown that apolipoprotein (apo) E is involved in maintaining PLTP in the active form, while the low-activity form is associated with apo AI. We have therefore investigated whether plasma apo AI, B and E concentrations are important determinants of plasma PLTP activity in type 2 diabetes, a condition associated with increased plasma PLTP activity. Methods: Plasma PLTP activity was assayed by measuring the transfer of radiolabelled phosphatidylcholine from liposomes to HDL; apo AI and B by rate nephelometry and apo E by a 2-point turbidimetric assay. Results: Type 2 diabetic patients (n = 230) had higher PLTP activity than controls (n = 97) (2374 ± 628 nmol/mL/h versus 1862 ± 585 respectively, p < 0.01). They also had increased fasting triglyceride and low HDL. Plasma apo B (p < 0.01) and apo E (p < 0.05) were increased, whereas apo AI was reduced (p < 0.01). Univariate analysis showed that plasma PLTP activity correlated mainly with apolipoproteins AI and E. Stepwise regression analysis showed that apo E was the main determinant of plasma PLTP activity, accounting for 23% of its variability in the diabetic subjects and 8% in the controls respectively. Conclusions: The associations between plasma apo AI and E concentrations and PLTP activity suggest that these apolipoproteins are important regulators of PLTP activity in vivo. The increase in PUP activity in type 2 diabetes is partly related to the changes in these apolipoproteins. Copyright © 2006 John Wiley & Sons, Ltd.en_HK
dc.languageengen_HK
dc.publisherJohn Wiley & Sons Ltd. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/10009394en_HK
dc.relation.ispartofDiabetes/Metabolism Research and Reviewsen_HK
dc.rightsDiabetes - Metabolism: Research and Reviews. Copyright © John Wiley & Sons Ltd.en_HK
dc.subject.meshAdulten_HK
dc.subject.meshApolipoproteins E - blooden_HK
dc.subject.meshDiabetes Mellitus, Type 2 - blooden_HK
dc.subject.meshFemaleen_HK
dc.subject.meshHumansen_HK
dc.subject.meshMaleen_HK
dc.subject.meshMiddle Ageden_HK
dc.subject.meshPhospholipid Transfer Proteins - blooden_HK
dc.subject.meshReference Valuesen_HK
dc.subject.meshRegression Analysisen_HK
dc.titlePlasma apolipoprotein E concentration is an important determinant of phospholipid transfer protein activity in type 2 diabetes mellitusen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1520-7552&volume=22&spage=307&epage=12&date=2006&atitle=Plasma+apolipoprotein+E+concentration+is+an+important+determinant+of+phospholipid+transfer+protein+activity+in+type+2+diabetes+mellitusen_HK
dc.identifier.emailTan, KCB:kcbtan@hku.hken_HK
dc.identifier.authorityTan, KCB=rp00402en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1002/dmrr.616en_HK
dc.identifier.pmid16389649-
dc.identifier.scopuseid_2-s2.0-33746081685en_HK
dc.identifier.hkuros113731en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-33746081685&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume22en_HK
dc.identifier.issue4en_HK
dc.identifier.spage307en_HK
dc.identifier.epage312en_HK
dc.identifier.isiWOS:000239227500006-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridTan, KCB=8082703100en_HK
dc.identifier.scopusauthoridShiu, SWM=7005550652en_HK
dc.identifier.scopusauthoridWong, Y=24073787400en_HK
dc.identifier.scopusauthoridWong, WK=12753634200en_HK
dc.identifier.scopusauthoridTam, S=7202037323en_HK

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