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Article: Aberrant promoter methylation in Chinese patients with non-small cell lung cancer: Patterns in primary tumors and potential diagnostic application in bronchoalevolar lavage

TitleAberrant promoter methylation in Chinese patients with non-small cell lung cancer: Patterns in primary tumors and potential diagnostic application in bronchoalevolar lavage
Authors
Issue Date2002
PublisherAmerican Association for Cancer Research.
Citation
Clinical Cancer Research, 2002, v. 8 n. 12, p. 3741-3746 How to Cite?
AbstractThis study was aimed at defining patterns of aberrant gene methylation in non-small cell lung cancer (NSCLC) in Chinese patients and its use in detecting cancer cells in bronchoalveolar lavage (BAL). The methylation-specific PCR (MSP) was used to study methylation of the p16, retinoic acid receptor-β (RARβ), death-associated protein (DAP) kinase, and O6-methylguanine-DNA-methyltransferase (MGMT) genes in 75 NSCLCs [44 adenocarcinomas and 31 squamous cell carcinomas (SCCs)] and 68 BALs from suspected lung cancers. More females had adenocarcinoma than SCC (11 of 44 versus 2 of 31, P = 0.04). Aberrant methylation in at least one gene was found in 63 of 75 (84%) NSCLCs. p16, RARβ, DAP kinase, and MGMT methylation was similar in adenocarcinoma and SCC. However, females with NSCLC showed more frequent p16 methylation than males (12 of 13 versus 36 of 62, P = 0.02), because of more frequent p16 methylation in female adenocarcinomas (10 of 11 versus 17 of 33, P = 0.02). This sexual difference was not observed in RARβ, DAP kinase, and MGMT. At 92%, the frequency of p16 methylation in Chinese female NSCLC is one of the highest known. For BAL, MSP and cytological analysis showed concordant and discordant results in 25 of 68 and 43 of 68 samples. Of 41 MSP+/cytology-cases, 35 were eventually shown to have malignant lung lesions, 4 were at high risk but had no evidence of lung cancer, and 2 were lost to follow-up. There were two MSP-/cytology+ cases. Frequent gene methylations were seen in Chinese NSCLC patients. More frequent p16 methylation was seen in female patients. MSP is a useful molecular adjunct for cancer cell detection in BAL samples.
Persistent Identifierhttp://hdl.handle.net/10722/77326
ISSN
2015 Impact Factor: 8.738
2015 SCImago Journal Rankings: 5.314
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorChan, ECen_HK
dc.contributor.authorLam, SYen_HK
dc.contributor.authorTsang, KWen_HK
dc.contributor.authorLam, Ben_HK
dc.contributor.authorHo, JCMen_HK
dc.contributor.authorFu, KHen_HK
dc.contributor.authorLam, WKen_HK
dc.contributor.authorKwong, YLen_HK
dc.date.accessioned2010-09-06T07:30:42Z-
dc.date.available2010-09-06T07:30:42Z-
dc.date.issued2002en_HK
dc.identifier.citationClinical Cancer Research, 2002, v. 8 n. 12, p. 3741-3746en_HK
dc.identifier.issn1078-0432en_HK
dc.identifier.urihttp://hdl.handle.net/10722/77326-
dc.description.abstractThis study was aimed at defining patterns of aberrant gene methylation in non-small cell lung cancer (NSCLC) in Chinese patients and its use in detecting cancer cells in bronchoalveolar lavage (BAL). The methylation-specific PCR (MSP) was used to study methylation of the p16, retinoic acid receptor-β (RARβ), death-associated protein (DAP) kinase, and O6-methylguanine-DNA-methyltransferase (MGMT) genes in 75 NSCLCs [44 adenocarcinomas and 31 squamous cell carcinomas (SCCs)] and 68 BALs from suspected lung cancers. More females had adenocarcinoma than SCC (11 of 44 versus 2 of 31, P = 0.04). Aberrant methylation in at least one gene was found in 63 of 75 (84%) NSCLCs. p16, RARβ, DAP kinase, and MGMT methylation was similar in adenocarcinoma and SCC. However, females with NSCLC showed more frequent p16 methylation than males (12 of 13 versus 36 of 62, P = 0.02), because of more frequent p16 methylation in female adenocarcinomas (10 of 11 versus 17 of 33, P = 0.02). This sexual difference was not observed in RARβ, DAP kinase, and MGMT. At 92%, the frequency of p16 methylation in Chinese female NSCLC is one of the highest known. For BAL, MSP and cytological analysis showed concordant and discordant results in 25 of 68 and 43 of 68 samples. Of 41 MSP+/cytology-cases, 35 were eventually shown to have malignant lung lesions, 4 were at high risk but had no evidence of lung cancer, and 2 were lost to follow-up. There were two MSP-/cytology+ cases. Frequent gene methylations were seen in Chinese NSCLC patients. More frequent p16 methylation was seen in female patients. MSP is a useful molecular adjunct for cancer cell detection in BAL samples.en_HK
dc.languageengen_HK
dc.publisherAmerican Association for Cancer Research.en_HK
dc.relation.ispartofClinical Cancer Researchen_HK
dc.subject.meshCalcium-Calmodulin-Dependent Protein Kinases - genetics-
dc.subject.meshCarcinoma, Non-Small-Cell Lung - genetics-
dc.subject.meshCyclin-Dependent Kinase Inhibitor p16 - genetics-
dc.subject.meshO(6)-Methylguanine-DNA Methyltransferase - genetics-
dc.subject.meshPromoter Regions, Genetic - genetics-
dc.titleAberrant promoter methylation in Chinese patients with non-small cell lung cancer: Patterns in primary tumors and potential diagnostic application in bronchoalevolar lavageen_HK
dc.typeArticleen_HK
dc.identifier.emailHo, JCM:jhocm@hku.hken_HK
dc.identifier.emailKwong, YL:ylkwong@hku.hken_HK
dc.identifier.authorityHo, JCM=rp00258en_HK
dc.identifier.authorityKwong, YL=rp00358en_HK
dc.description.naturelink_to_OA_fulltext-
dc.identifier.pmid12473584-
dc.identifier.scopuseid_2-s2.0-0036899938en_HK
dc.identifier.hkuros108224en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0036899938&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume8en_HK
dc.identifier.issue12en_HK
dc.identifier.spage3741en_HK
dc.identifier.epage3746en_HK
dc.identifier.isiWOS:000179673400013-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridChan, EC=7401994120en_HK
dc.identifier.scopusauthoridLam, SY=7402279461en_HK
dc.identifier.scopusauthoridTsang, KW=7201555024en_HK
dc.identifier.scopusauthoridLam, B=9246012800en_HK
dc.identifier.scopusauthoridHo, JCM=7402649981en_HK
dc.identifier.scopusauthoridFu, KH=7202283800en_HK
dc.identifier.scopusauthoridLam, WK=7203021937en_HK
dc.identifier.scopusauthoridKwong, YL=7102818954en_HK

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