File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: The use of IgH fingerprinting and ASO-dependent PCR for the investigation of residual disease (MRD) in ALL

TitleThe use of IgH fingerprinting and ASO-dependent PCR for the investigation of residual disease (MRD) in ALL
Authors
Issue Date1996
PublisherBlackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/BJH
Citation
British Journal Of Haematology, 1996, v. 92 n. 1, p. 104-115 How to Cite?
AbstractIn acute lymphoblastic leukaemia (ALL), investigation of minimal residual disease by conventional morphology and immunology fails to detect levels of residual disease of <1 leukaemic in 10-100 normal cells. The use of polymerase chain reaction (PCR) to exploit the diversity of the complementarity determining region (CDR) and immunoglobulin variable heavy chain (VH) family specific usage has greatly improved the sensitivity up to one leukaemic cell in 105-106 normal bone marrow cells. Here we report on a prospective study of 14 patients with ALL of B-cell lineage by using a combined PCR approach which estimates levels of disease between 1:103 and 1:105. The sequential use of allele-specific oligoprimer (ASO) independent tests (using framework 1, FR1 and 3, FR3 primers with a JH consensus primer, sensivity up to 1:5 x 103) and ASO-dependent PCR (sensitivity up to 1:105.) assays were applied to 64 bone marrow (BM) follow-up samples in a sequential array of tests. Results presented in this study indicate high concordance of MRD among different tests for samples with level of residual disease > 1:5 x 103. Consequently, samples positive by the FR1 and FR3 fingerprinting tests were confirmed by the more sensitive ASO-denpendent tests, as expected. However, the ASO-dependent assays revealed levels of disease undetected by the FR1 and FR3 test. Although a higher level of sensitivity is provided by the ASO-dependent tests, the FR1 and FR3 fingerprinting tests allow MRD investigation in patients with oligoclonal B cell proliferations, CDR3 region of size < 15bp or with ASO primers unsuitable for PCR investigation on technical grounds (i.e. background signal). If a sequential order of investigation from less (e.g. FR1 and FR3 fingerprinting) to more sensitive tests (ASO-dependent) is applied, an indirect estimate of MRD is obtained for patients with level of disease < 1:103.
Persistent Identifierhttp://hdl.handle.net/10722/77233
ISSN
2015 Impact Factor: 5.401
2015 SCImago Journal Rankings: 2.313
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorChim, JCSen_HK
dc.contributor.authorCoyle, LAen_HK
dc.contributor.authorYaxley, JCen_HK
dc.contributor.authorColeSinclair, MFen_HK
dc.contributor.authorCannell, PKen_HK
dc.contributor.authorHoffbrand, VAen_HK
dc.contributor.authorForoni, Len_HK
dc.date.accessioned2010-09-06T07:29:42Z-
dc.date.available2010-09-06T07:29:42Z-
dc.date.issued1996en_HK
dc.identifier.citationBritish Journal Of Haematology, 1996, v. 92 n. 1, p. 104-115en_HK
dc.identifier.issn0007-1048en_HK
dc.identifier.urihttp://hdl.handle.net/10722/77233-
dc.description.abstractIn acute lymphoblastic leukaemia (ALL), investigation of minimal residual disease by conventional morphology and immunology fails to detect levels of residual disease of <1 leukaemic in 10-100 normal cells. The use of polymerase chain reaction (PCR) to exploit the diversity of the complementarity determining region (CDR) and immunoglobulin variable heavy chain (VH) family specific usage has greatly improved the sensitivity up to one leukaemic cell in 105-106 normal bone marrow cells. Here we report on a prospective study of 14 patients with ALL of B-cell lineage by using a combined PCR approach which estimates levels of disease between 1:103 and 1:105. The sequential use of allele-specific oligoprimer (ASO) independent tests (using framework 1, FR1 and 3, FR3 primers with a JH consensus primer, sensivity up to 1:5 x 103) and ASO-dependent PCR (sensitivity up to 1:105.) assays were applied to 64 bone marrow (BM) follow-up samples in a sequential array of tests. Results presented in this study indicate high concordance of MRD among different tests for samples with level of residual disease > 1:5 x 103. Consequently, samples positive by the FR1 and FR3 fingerprinting tests were confirmed by the more sensitive ASO-denpendent tests, as expected. However, the ASO-dependent assays revealed levels of disease undetected by the FR1 and FR3 test. Although a higher level of sensitivity is provided by the ASO-dependent tests, the FR1 and FR3 fingerprinting tests allow MRD investigation in patients with oligoclonal B cell proliferations, CDR3 region of size < 15bp or with ASO primers unsuitable for PCR investigation on technical grounds (i.e. background signal). If a sequential order of investigation from less (e.g. FR1 and FR3 fingerprinting) to more sensitive tests (ASO-dependent) is applied, an indirect estimate of MRD is obtained for patients with level of disease < 1:103.en_HK
dc.languageengen_HK
dc.publisherBlackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/BJHen_HK
dc.relation.ispartofBritish Journal of Haematologyen_HK
dc.rightsBritish Journal of Haematology. Copyright © Blackwell Publishing Ltd.en_HK
dc.subject.meshAdolescenten_HK
dc.subject.meshAdulten_HK
dc.subject.meshAllelesen_HK
dc.subject.meshAntibodies, Neoplasm - geneticsen_HK
dc.subject.meshBase Sequenceen_HK
dc.subject.meshBurkitt Lymphoma - diagnosisen_HK
dc.subject.meshChilden_HK
dc.subject.meshChild, Preschoolen_HK
dc.subject.meshDNA Fingerprinting - methodsen_HK
dc.subject.meshFemaleen_HK
dc.subject.meshFollow-Up Studiesen_HK
dc.subject.meshHumansen_HK
dc.subject.meshImmunoglobulin Heavy Chains - geneticsen_HK
dc.subject.meshImmunoglobulin Variable Region - geneticsen_HK
dc.subject.meshMaleen_HK
dc.subject.meshMolecular Sequence Dataen_HK
dc.subject.meshNeoplasm, Residual - drug therapyen_HK
dc.subject.meshPolymerase Chain Reaction - methodsen_HK
dc.subject.meshProspective Studiesen_HK
dc.subject.meshSensitivity and Specificityen_HK
dc.titleThe use of IgH fingerprinting and ASO-dependent PCR for the investigation of residual disease (MRD) in ALLen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0007-1048&volume=92&spage=104&epage=115&date=1996&atitle=The+use+of+IgH+fingerprinting+and+ASO-dependent+PCR+for+the+investigation+of+residual+disease+(MRD)+in+ALL.en_HK
dc.identifier.emailChim, JCS:jcschim@hku.hken_HK
dc.identifier.authorityChim, JCS=rp00408en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1046/j.1365-2141.1996.289831.x-
dc.identifier.pmid8562380-
dc.identifier.scopuseid_2-s2.0-0030040436en_HK
dc.identifier.hkuros25646en_HK
dc.identifier.hkuros13341-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0030040436&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume92en_HK
dc.identifier.issue1en_HK
dc.identifier.spage104en_HK
dc.identifier.epage115en_HK
dc.identifier.isiWOS:A1996TN89300015-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridChim, JCS=7004597253en_HK
dc.identifier.scopusauthoridCoyle, LA=6701910626en_HK
dc.identifier.scopusauthoridYaxley, JC=6603510974en_HK
dc.identifier.scopusauthoridColeSinclair, MF=6602634669en_HK
dc.identifier.scopusauthoridCannell, PK=6701709012en_HK
dc.identifier.scopusauthoridHoffbrand, VA=6603600054en_HK
dc.identifier.scopusauthoridForoni, L=7005279817en_HK

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats