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Article: Proliferating cell nuclear antigen (PCNA) as a proliferative marker during embryonic and adult zebrafish hematopoiesis

TitleProliferating cell nuclear antigen (PCNA) as a proliferative marker during embryonic and adult zebrafish hematopoiesis
Authors
KeywordsHematopoiesis
ICM
Kidneys
PCNA
Zebrafish
Issue Date2005
PublisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00418/index.htm
Citation
Histochemistry And Cell Biology, 2005, v. 124 n. 2, p. 105-111 How to Cite?
AbstractWe investigated the expression of proliferative cell nuclear antigen (PCNA) in zebrafish to delineate the proliferative hematopoietic component during adult and embryonic hematopoiesis. Immunostaining for PCNA and enhanced green fluorescence protein (eGFP) was performed in wild-type and fli1-eGFP (endothelial marker) and gata1-eGFP (erythroid cell marker) transgenic fish. Expression of PCNA mRNA was examined in wild-type and chordin morphant embryos. In adult zebrafish kidney, the renal tubules are surrounded by endothelial cells and it is separated into hematopoietic and excretory compartments. PCNA was expressed in hematopoietic progenitor cells but not in mature neutrophils, eosinophils or erythroid cells. Some PCNA+ cells are scattered in the hematopoietic compartment of the kidney while others are closely associated with renal tubular cells. PCNA was also expressed in spermatogonial stem cells and intestine crypts, consistent with its role in cell proliferation and DNA synthesis. In embryos, PCNA is expressed in the brain, spinal cord and intermediate cell mass (ICM) at 24 h-post fertilization. In chordin morphants, PCNA is significantly upregulated in the expanded ICM. Therefore, PCNA can be used to mark cell proliferation in zebrafish hematopoietic tissues and to identify a population of progenitor cells whose significance would have to be further investigated. © Springer-Verlag 2005.
Persistent Identifierhttp://hdl.handle.net/10722/77221
ISSN
2015 Impact Factor: 2.78
2015 SCImago Journal Rankings: 1.287
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLeung, AYHen_HK
dc.contributor.authorLeung, JCKen_HK
dc.contributor.authorChan, LYYen_HK
dc.contributor.authorMa, ESKen_HK
dc.contributor.authorKwan, TTFen_HK
dc.contributor.authorLai, KNen_HK
dc.contributor.authorMeng, Aen_HK
dc.contributor.authorLiang, Ren_HK
dc.date.accessioned2010-09-06T07:29:34Z-
dc.date.available2010-09-06T07:29:34Z-
dc.date.issued2005en_HK
dc.identifier.citationHistochemistry And Cell Biology, 2005, v. 124 n. 2, p. 105-111en_HK
dc.identifier.issn0948-6143en_HK
dc.identifier.urihttp://hdl.handle.net/10722/77221-
dc.description.abstractWe investigated the expression of proliferative cell nuclear antigen (PCNA) in zebrafish to delineate the proliferative hematopoietic component during adult and embryonic hematopoiesis. Immunostaining for PCNA and enhanced green fluorescence protein (eGFP) was performed in wild-type and fli1-eGFP (endothelial marker) and gata1-eGFP (erythroid cell marker) transgenic fish. Expression of PCNA mRNA was examined in wild-type and chordin morphant embryos. In adult zebrafish kidney, the renal tubules are surrounded by endothelial cells and it is separated into hematopoietic and excretory compartments. PCNA was expressed in hematopoietic progenitor cells but not in mature neutrophils, eosinophils or erythroid cells. Some PCNA+ cells are scattered in the hematopoietic compartment of the kidney while others are closely associated with renal tubular cells. PCNA was also expressed in spermatogonial stem cells and intestine crypts, consistent with its role in cell proliferation and DNA synthesis. In embryos, PCNA is expressed in the brain, spinal cord and intermediate cell mass (ICM) at 24 h-post fertilization. In chordin morphants, PCNA is significantly upregulated in the expanded ICM. Therefore, PCNA can be used to mark cell proliferation in zebrafish hematopoietic tissues and to identify a population of progenitor cells whose significance would have to be further investigated. © Springer-Verlag 2005.en_HK
dc.languageengen_HK
dc.publisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00418/index.htmen_HK
dc.relation.ispartofHistochemistry and Cell Biologyen_HK
dc.subjectHematopoiesisen_HK
dc.subjectICMen_HK
dc.subjectKidneysen_HK
dc.subjectPCNAen_HK
dc.subjectZebrafishen_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshAnimals, Genetically Modifieden_HK
dc.subject.meshBiological Markers - metabolismen_HK
dc.subject.meshCell Proliferationen_HK
dc.subject.meshEmbryo, Nonmammalian - cytology - metabolismen_HK
dc.subject.meshGreen Fluorescent Proteins - metabolismen_HK
dc.subject.meshHematopoiesis, Extramedullary - physiologyen_HK
dc.subject.meshHematopoietic Stem Cells - cytology - metabolismen_HK
dc.subject.meshImmunoenzyme Techniquesen_HK
dc.subject.meshIn Situ Hybridizationen_HK
dc.subject.meshKidney - cytology - metabolismen_HK
dc.subject.meshMaleen_HK
dc.subject.meshProliferating Cell Nuclear Antigen - genetics - metabolismen_HK
dc.subject.meshRNA, Messenger - metabolismen_HK
dc.subject.meshZebrafishen_HK
dc.titleProliferating cell nuclear antigen (PCNA) as a proliferative marker during embryonic and adult zebrafish hematopoiesisen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0948-6143&volume=124&issue=2&spage=105&epage=11&date=2005&atitle=Proliferating+cell+nuclear+antigen+(PCNA)+as+a+proliferative+marker+during+embryonic+and+adult+zebrafish+hematopoiesisen_HK
dc.identifier.emailLeung, AYH: ayhleung@hku.hken_HK
dc.identifier.emailLeung, JCK: jckleung@hku.hken_HK
dc.identifier.emailLai, KN: knlai@hku.hken_HK
dc.identifier.emailLiang, R: rliang@hku.hken_HK
dc.identifier.authorityLeung, AYH=rp00265en_HK
dc.identifier.authorityLeung, JCK=rp00448en_HK
dc.identifier.authorityLai, KN=rp00324en_HK
dc.identifier.authorityLiang, R=rp00345en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1007/s00418-005-0003-2en_HK
dc.identifier.pmid16028068-
dc.identifier.scopuseid_2-s2.0-26444545046en_HK
dc.identifier.hkuros122756en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-26444545046&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume124en_HK
dc.identifier.issue2en_HK
dc.identifier.spage105en_HK
dc.identifier.epage111en_HK
dc.identifier.isiWOS:000232530700002-
dc.publisher.placeGermanyen_HK
dc.identifier.scopusauthoridLeung, AYH=7403012668en_HK
dc.identifier.scopusauthoridLeung, JCK=7202180349en_HK
dc.identifier.scopusauthoridChan, LYY=8108378300en_HK
dc.identifier.scopusauthoridMa, ESK=7202039934en_HK
dc.identifier.scopusauthoridKwan, TTF=8776810800en_HK
dc.identifier.scopusauthoridLai, KN=7402135706en_HK
dc.identifier.scopusauthoridMeng, A=7006106872en_HK
dc.identifier.scopusauthoridLiang, R=26643224900en_HK
dc.identifier.citeulike10829357-

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