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Article: Plasma membrane cholesterol: A possible barrier to intracellular oxygen in normal and mutant CHO cells defective in cholesterol metabolism

TitlePlasma membrane cholesterol: A possible barrier to intracellular oxygen in normal and mutant CHO cells defective in cholesterol metabolism
Authors
Issue Date2003
PublisherAmerican Chemical Society. The Journal's web site is located at http://pubs.acs.org/biochemistry
Citation
Biochemistry, 2003, v. 42 n. 1, p. 23-29 How to Cite?
AbstractThe effect of the cholesterol content of the plasma membrane on the intracellular concentration of oxygen in Chinese hamster ovary (CHO) cells and their mutants was investigated by EPR oximetry. Total and free cholesterol content was significantly higher in 25 RA CHO cells as compared to wild-type and M 19 CHO cells, with most of the free cholesterol in normal and mutant CHO cells located in the plasma membrane. The plasma membrane cholesterol content also was altered by various biochemical means, and the effect on the oxygen gradient was studied. Comparing the three cell lines, the gradient was larger with increased content of cholesterol in the plasma cell membrane. This result also is supported by an additional increase in the oxygen gradients with the incorporation of additional cholesterol in the plasma membrane and a decrease in the oxygen gradient when the cholesterol was depleted from the plasma membrane. The results indicate that the concentration of cholesterol in the plasma membrane can be an important factor for the magnitude of the oxygen gradient observed across the cell membrane.
Persistent Identifierhttp://hdl.handle.net/10722/77208
ISSN
2015 Impact Factor: 2.876
2015 SCImago Journal Rankings: 1.769
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorKhan, Nen_HK
dc.contributor.authorShen, Jen_HK
dc.contributor.authorChang, TYen_HK
dc.contributor.authorChang, CCen_HK
dc.contributor.authorFung, PCWen_HK
dc.contributor.authorGrinberg, Oen_HK
dc.contributor.authorDemidenko, Een_HK
dc.contributor.authorSwartz, Hen_HK
dc.date.accessioned2010-09-06T07:29:26Z-
dc.date.available2010-09-06T07:29:26Z-
dc.date.issued2003en_HK
dc.identifier.citationBiochemistry, 2003, v. 42 n. 1, p. 23-29en_HK
dc.identifier.issn0006-2960en_HK
dc.identifier.urihttp://hdl.handle.net/10722/77208-
dc.description.abstractThe effect of the cholesterol content of the plasma membrane on the intracellular concentration of oxygen in Chinese hamster ovary (CHO) cells and their mutants was investigated by EPR oximetry. Total and free cholesterol content was significantly higher in 25 RA CHO cells as compared to wild-type and M 19 CHO cells, with most of the free cholesterol in normal and mutant CHO cells located in the plasma membrane. The plasma membrane cholesterol content also was altered by various biochemical means, and the effect on the oxygen gradient was studied. Comparing the three cell lines, the gradient was larger with increased content of cholesterol in the plasma cell membrane. This result also is supported by an additional increase in the oxygen gradients with the incorporation of additional cholesterol in the plasma membrane and a decrease in the oxygen gradient when the cholesterol was depleted from the plasma membrane. The results indicate that the concentration of cholesterol in the plasma membrane can be an important factor for the magnitude of the oxygen gradient observed across the cell membrane.en_HK
dc.languageengen_HK
dc.publisherAmerican Chemical Society. The Journal's web site is located at http://pubs.acs.org/biochemistryen_HK
dc.relation.ispartofBiochemistryen_HK
dc.titlePlasma membrane cholesterol: A possible barrier to intracellular oxygen in normal and mutant CHO cells defective in cholesterol metabolismen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0006-2960&volume=42&issue=1&spage=23&epage=29&date=2003&atitle=Plasma+membrane+cholesterol:+A+possible+barrier+to+intracellular+oxygen+in+normal+and+mutant+CHO+cells+defective+in+cholesterol+metabolismen_HK
dc.identifier.emailShen, J: shenjg@hku.hken_HK
dc.identifier.authorityShen, J=rp00487en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1021/bi026039ten_HK
dc.identifier.pmid12515536-
dc.identifier.scopuseid_2-s2.0-0347926502en_HK
dc.identifier.hkuros84032en_HK
dc.identifier.hkuros95066-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0347926502&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume42en_HK
dc.identifier.issue1en_HK
dc.identifier.spage23en_HK
dc.identifier.epage29en_HK
dc.identifier.isiWOS:000180324300004-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridKhan, N=7401797460en_HK
dc.identifier.scopusauthoridShen, J=7404929947en_HK
dc.identifier.scopusauthoridChang, TY=7404726013en_HK
dc.identifier.scopusauthoridChang, CC=36134211000en_HK
dc.identifier.scopusauthoridFung, PCW=7101613315en_HK
dc.identifier.scopusauthoridGrinberg, O=7003675552en_HK
dc.identifier.scopusauthoridDemidenko, E=7003550138en_HK
dc.identifier.scopusauthoridSwartz, H=7102010298en_HK

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