File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Resolution of chronic hepatitis B and anti-HBs seroconversion in humans by adoptive transfer of immunity to hepatitis B core antigen

TitleResolution of chronic hepatitis B and anti-HBs seroconversion in humans by adoptive transfer of immunity to hepatitis B core antigen
Authors
Issue Date2002
PublisherWB Saunders Co. The Journal's web site is located at http://www.elsevier.com/locate/gastro
Citation
Gastroenterology, 2002, v. 122 n. 3, p. 614-624 How to Cite?
AbstractBackground & Aims: Impaired T-cell reactivity is believed to be the dominant cause of chronic hepatitis B virus (HBV) infection. We characterized HBV-specific T-cell responses in chronic hepatitis B surface antigen carriers who received bone marrow from HLA-identical donors with natural immunity to HBV and seroconverted to antibody to hepatitis B surface antigen. Methods: T-cell reactivity to HBV antigens and peptides was assessed in a proliferation assay, the frequency of HBV core- and surface-specific T cells was quantified directly by ELISPOT assays, and T-cell subsets were analyzed by flow cytometry. Results: CD4+ T-cell reactivity to HBV core was common in bone marrow donors and the corresponding recipients after hepatitis B surface antigen clearance, whereas none reacted to surface, pre-S1, or pre-S2 antigens. Furthermore, CD4+ T cells from donor/recipient pairs recognized similar epitopes on hepatitis B core antigen; using polymerase chain reaction for the Y chromosome, the recipients' CD4+ T lymphocytes were confirmed to be of donor origin. The frequency of core-specific CD4+ and CD8+ T cells was several-fold higher than those specific for surface antigen. Conclusions: This study provides the first evidence in humans that transfer of hepatitis B core antigen-reactive T cells is associated with resolution of chronic HBV infection. Therapeutic immunization with HBV core gene or protein deserves further investigation in patients with chronic hepatitis B.
Persistent Identifierhttp://hdl.handle.net/10722/77136
ISSN
2015 Impact Factor: 18.187
2015 SCImago Journal Rankings: 7.170
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLau, GKKen_HK
dc.contributor.authorSuri, Den_HK
dc.contributor.authorLiang, Ren_HK
dc.contributor.authorRigopoulou, EIen_HK
dc.contributor.authorThomas, MGen_HK
dc.contributor.authorMullerova, Ien_HK
dc.contributor.authorNanji, Aen_HK
dc.contributor.authorYuen, Sen_HK
dc.contributor.authorWilliams, Ren_HK
dc.contributor.authorNaoumov, NVen_HK
dc.date.accessioned2010-09-06T07:28:39Z-
dc.date.available2010-09-06T07:28:39Z-
dc.date.issued2002en_HK
dc.identifier.citationGastroenterology, 2002, v. 122 n. 3, p. 614-624en_HK
dc.identifier.issn0016-5085en_HK
dc.identifier.urihttp://hdl.handle.net/10722/77136-
dc.description.abstractBackground & Aims: Impaired T-cell reactivity is believed to be the dominant cause of chronic hepatitis B virus (HBV) infection. We characterized HBV-specific T-cell responses in chronic hepatitis B surface antigen carriers who received bone marrow from HLA-identical donors with natural immunity to HBV and seroconverted to antibody to hepatitis B surface antigen. Methods: T-cell reactivity to HBV antigens and peptides was assessed in a proliferation assay, the frequency of HBV core- and surface-specific T cells was quantified directly by ELISPOT assays, and T-cell subsets were analyzed by flow cytometry. Results: CD4+ T-cell reactivity to HBV core was common in bone marrow donors and the corresponding recipients after hepatitis B surface antigen clearance, whereas none reacted to surface, pre-S1, or pre-S2 antigens. Furthermore, CD4+ T cells from donor/recipient pairs recognized similar epitopes on hepatitis B core antigen; using polymerase chain reaction for the Y chromosome, the recipients' CD4+ T lymphocytes were confirmed to be of donor origin. The frequency of core-specific CD4+ and CD8+ T cells was several-fold higher than those specific for surface antigen. Conclusions: This study provides the first evidence in humans that transfer of hepatitis B core antigen-reactive T cells is associated with resolution of chronic HBV infection. Therapeutic immunization with HBV core gene or protein deserves further investigation in patients with chronic hepatitis B.en_HK
dc.languageengen_HK
dc.publisherWB Saunders Co. The Journal's web site is located at http://www.elsevier.com/locate/gastroen_HK
dc.relation.ispartofGastroenterologyen_HK
dc.subject.meshAdolescenten_HK
dc.subject.meshAdulten_HK
dc.subject.meshBone Marrow Transplantationen_HK
dc.subject.meshCD4-Positive T-Lymphocytes - cytology - immunology - virologyen_HK
dc.subject.meshCD8-Positive T-Lymphocytes - cytology - immunology - virologyen_HK
dc.subject.meshCell Division - immunologyen_HK
dc.subject.meshFemaleen_HK
dc.subject.meshFlow Cytometryen_HK
dc.subject.meshHepatitis B Antibodies - blooden_HK
dc.subject.meshHepatitis B Core Antigens - immunologyen_HK
dc.subject.meshHepatitis B, Chronic - immunology - therapyen_HK
dc.subject.meshHumansen_HK
dc.subject.meshImmunotherapy, Adoptiveen_HK
dc.subject.meshLymphocyte Activation - immunologyen_HK
dc.subject.meshMaleen_HK
dc.subject.meshMiddle Ageden_HK
dc.titleResolution of chronic hepatitis B and anti-HBs seroconversion in humans by adoptive transfer of immunity to hepatitis B core antigenen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0016-5085&volume=122&issue=3&spage=614&epage=624&date=2002&atitle=Resolution+of+chronic+hepatitis+B+and+anti-HBs+seroconversion+in+humans+by+adoptive+transfer+of+immunity+to+hepatitis+B+core+antigenen_HK
dc.identifier.emailLiang, R:rliang@hku.hken_HK
dc.identifier.authorityLiang, R=rp00345en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.pmid11874993-
dc.identifier.scopuseid_2-s2.0-0036176535en_HK
dc.identifier.hkuros67750en_HK
dc.identifier.hkuros67538-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0036176535&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume122en_HK
dc.identifier.issue3en_HK
dc.identifier.spage614en_HK
dc.identifier.epage624en_HK
dc.identifier.isiWOS:000174056900006-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridLau, GKK=7102301257en_HK
dc.identifier.scopusauthoridSuri, D=15749573300en_HK
dc.identifier.scopusauthoridLiang, R=26643224900en_HK
dc.identifier.scopusauthoridRigopoulou, EI=6602370445en_HK
dc.identifier.scopusauthoridThomas, MG=34572732200en_HK
dc.identifier.scopusauthoridMullerova, I=15749530400en_HK
dc.identifier.scopusauthoridNanji, A=35885060300en_HK
dc.identifier.scopusauthoridYuen, S=8323342200en_HK
dc.identifier.scopusauthoridWilliams, R=35400042900en_HK
dc.identifier.scopusauthoridNaoumov, NV=7006460467en_HK

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats