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Article: Intracerebroventricular injection of a neuropeptide Y-Y1 receptor agonist increases while BIBP3226, a Y1 antagonist, reduces the infarct volume following transient middle cerebral artery occlusion in rats

TitleIntracerebroventricular injection of a neuropeptide Y-Y1 receptor agonist increases while BIBP3226, a Y1 antagonist, reduces the infarct volume following transient middle cerebral artery occlusion in rats
Authors
Issue Date2003
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/neuroscience
Citation
Neuroscience, 2003, v. 116 n. 1, p. 119-126 How to Cite?
AbstractRecent studies using middle cerebral artery occlusion in the rat have suggested a role of neuropeptide Y in ischemic pathophysiology. In this study, we investigated the effects of an i.c.v. injection of a neuropeptide Y-Y2 receptor agonist, neuropeptide Y 3-36, a Y1 receptor agonist, [Leu31,Pro34]-neuropeptide Y, or a Y1 receptor antagonist, BIBP3226, on infarct volume and hemodynamic parameters following middle cerebral artery occlusion. Adult male Sprague-Dawley rats were subjected to transient middle cerebral artery occlusion for 2 h. A single i.c.v. injection of neuropeptide Y 3-36 (15 μg/kg), [Leu31,Pro34]-neuropeptide Y (30 μg/kg), or BIBP3226 (5, 15, or 45 μg/kg) was given at 30 min of ischemia. Blood pressure, heart rate, and regional cerebral perfusion were monitored during ischemia and reperfusion. The rats were decapitated after 70 h of reperfusion, and their brains were cut into 2-mm-thick coronal slices before reaction with a 2% solution of 2,3,5-triphenyltetrazolium chloride to reveal the infarct. When compared with an infarct volume of 17.4±4.4% of the ipsilateral hemisphere following injection of neuropeptide Y 3-36, administration of the Y1 receptor analogs significantly modified the infarct volume (ordinary one-way analysis of variance (ANOVA), P<0.0001). [Leu31,Pro34]-neuropeptide Y increased the infarct volume to 32.0±4.1% (Student-Newman-Keuls post-test, P<0.01), whereas BIBP3226 at 15 μg/kg decreased the infarct volume to 6.5±1.0% (post-test P<0.05). Although there was no major difference in the hemodynamic parameters among the groups, injection of [Leu31,Pro34]-neuropeptide Y tended to further reduce cerebral perfusion during ischemia, while injection of BIBP3226 at 15 μg/kg appeared to have the opposite effect. In addition to glutamate, calcium ion and nitric oxide, activation of the neuropeptide Y-Y1 receptors may mediate cerebral damage during focal ischemia. Conversely, inhibiting the Y1 receptors may protect the brain against ischemic injury. Further studies are warranted to confirm the neuroprotective potential of neuropeptide Y-Y1 receptor inhibition. © 2003 IBRO. Published by Elsevier Science Ltd. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/77122
ISSN
2015 Impact Factor: 3.231
2015 SCImago Journal Rankings: 1.768
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorChen, SHen_HK
dc.contributor.authorCheung, RTFen_HK
dc.date.accessioned2010-09-06T07:28:30Z-
dc.date.available2010-09-06T07:28:30Z-
dc.date.issued2003en_HK
dc.identifier.citationNeuroscience, 2003, v. 116 n. 1, p. 119-126en_HK
dc.identifier.issn0306-4522en_HK
dc.identifier.urihttp://hdl.handle.net/10722/77122-
dc.description.abstractRecent studies using middle cerebral artery occlusion in the rat have suggested a role of neuropeptide Y in ischemic pathophysiology. In this study, we investigated the effects of an i.c.v. injection of a neuropeptide Y-Y2 receptor agonist, neuropeptide Y 3-36, a Y1 receptor agonist, [Leu31,Pro34]-neuropeptide Y, or a Y1 receptor antagonist, BIBP3226, on infarct volume and hemodynamic parameters following middle cerebral artery occlusion. Adult male Sprague-Dawley rats were subjected to transient middle cerebral artery occlusion for 2 h. A single i.c.v. injection of neuropeptide Y 3-36 (15 μg/kg), [Leu31,Pro34]-neuropeptide Y (30 μg/kg), or BIBP3226 (5, 15, or 45 μg/kg) was given at 30 min of ischemia. Blood pressure, heart rate, and regional cerebral perfusion were monitored during ischemia and reperfusion. The rats were decapitated after 70 h of reperfusion, and their brains were cut into 2-mm-thick coronal slices before reaction with a 2% solution of 2,3,5-triphenyltetrazolium chloride to reveal the infarct. When compared with an infarct volume of 17.4±4.4% of the ipsilateral hemisphere following injection of neuropeptide Y 3-36, administration of the Y1 receptor analogs significantly modified the infarct volume (ordinary one-way analysis of variance (ANOVA), P<0.0001). [Leu31,Pro34]-neuropeptide Y increased the infarct volume to 32.0±4.1% (Student-Newman-Keuls post-test, P<0.01), whereas BIBP3226 at 15 μg/kg decreased the infarct volume to 6.5±1.0% (post-test P<0.05). Although there was no major difference in the hemodynamic parameters among the groups, injection of [Leu31,Pro34]-neuropeptide Y tended to further reduce cerebral perfusion during ischemia, while injection of BIBP3226 at 15 μg/kg appeared to have the opposite effect. In addition to glutamate, calcium ion and nitric oxide, activation of the neuropeptide Y-Y1 receptors may mediate cerebral damage during focal ischemia. Conversely, inhibiting the Y1 receptors may protect the brain against ischemic injury. Further studies are warranted to confirm the neuroprotective potential of neuropeptide Y-Y1 receptor inhibition. © 2003 IBRO. Published by Elsevier Science Ltd. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/neuroscienceen_HK
dc.relation.ispartofNeuroscienceen_HK
dc.rightsNeuroscience. Copyright © Elsevier BV.en_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshAnti-Anxiety Agents - administration & dosage - pharmacologyen_HK
dc.subject.meshArginine - administration & dosage - analogs & derivatives - pharmacologyen_HK
dc.subject.meshInfarction, Middle Cerebral Artery - drug therapy - metabolismen_HK
dc.subject.meshInjections, Intraventricularen_HK
dc.subject.meshMaleen_HK
dc.subject.meshNeuropeptide Y - adverse effects - analogs & derivatives - pharmacologyen_HK
dc.subject.meshPeptide Fragments - pharmacologyen_HK
dc.subject.meshRatsen_HK
dc.subject.meshRats, Sprague-Dawleyen_HK
dc.subject.meshReceptors, Neuropeptide Y - agonists - antagonists & inhibitors - metabolismen_HK
dc.titleIntracerebroventricular injection of a neuropeptide Y-Y1 receptor agonist increases while BIBP3226, a Y1 antagonist, reduces the infarct volume following transient middle cerebral artery occlusion in ratsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0306-4522&volume=116&spage=119&epage=126&date=2003&atitle=Intracerebroventricular+injection+of+a+neuropeptide+Y-Y1+receptor+agonist+increases+while+BIBP3226,+a+Y1+antagonist,+reduces+the+infarct+volume+following+transient+middle+cerebral+artery+occlusion+in+ratsen_HK
dc.identifier.emailCheung, RTF:rtcheung@hku.hken_HK
dc.identifier.authorityCheung, RTF=rp00434en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/S0306-4522(02)00576-6en_HK
dc.identifier.pmid12535945-
dc.identifier.scopuseid_2-s2.0-0037439157en_HK
dc.identifier.hkuros87554en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0037439157&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume116en_HK
dc.identifier.issue1en_HK
dc.identifier.spage119en_HK
dc.identifier.epage126en_HK
dc.identifier.isiWOS:000180738000016-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridChen, SH=12806098400en_HK
dc.identifier.scopusauthoridCheung, RTF=7202397498en_HK

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