File Download
There are no files associated with this item.
Links for fulltext
(May Require Subscription)
- Publisher Website: 10.1007/s00775-007-0237-7
- Scopus: eid_2-s2.0-34547404155
- PMID: 17503094
- WOS: WOS:000248414100011
- Find via
Supplementary
-
Bookmarks:
- CiteULike: 1
- Citations:
- Appears in Collections:
Article: A proteomic approach for the identification of bismuth-binding proteins in Helicobacter pylori
Title | A proteomic approach for the identification of bismuth-binding proteins in Helicobacter pylori |
---|---|
Authors | |
Keywords | Colloidal bismuth subcitrate Helicobacter pylori Immobilized-metal affinity chromatography Proteomics Reactive oxygen species |
Issue Date | 2007 |
Publisher | Springer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00775/index.htm |
Citation | Journal of Biological Inorganic Chemistry, 2007, v. 12 n. 6, p. 831-842 How to Cite? |
Abstract | Helicobacter pylori is a major human pathogen that can cause peptic ulcers and chronic gastritis. Bismuth-based triple or quadruple therapies are commonly recommended for the treatment of H. pylori infections. However, the molecular mechanisms underlying treatment with bismuth are currently not fully understood. We have conducted a detailed comparative proteomic analysis of H. pylori cells both before and after treatment with colloidal bismuth subcitrate (CBS). Eight proteins were found to be significantly upregulated or downregulated in the presence of CBS (20 μg mL -1). Bismuth-induced oxidative stress was confirmed by detecting higher levels of lipid hydroperoxide (approximately 1.8 times) and hemin (approximately 3.4 times), in whole cell extracts of bismuth-treated H. pylori cells, compared with those from untreated cells. The presence of bismuth also led to an approximately eightfold decrease in cellular protease activities. Using immobilized-bismuth affinity chromatography, we isolated and subsequently identified seven bismuth-binding proteins from H. pylori cell extracts. The intracellular levels of four of these proteins (HspA, HspB, NapA and TsaA) were influenced by the addition of CBS, which strongly suggests that they interact directly with bismuth. The other bismuth-interacting proteins identified were two enzymes (fumarase and the urease subunit UreB), and a translational factor (Ef-Tu). Our data suggest that the inhibition of proteases, modulation of cellular oxidative stress and interference with nickel homeostasis may be key processes underlying the molecular mechanism of bismuth's actions against H. pylori. © 2007 SBIC. |
Persistent Identifier | http://hdl.handle.net/10722/77087 |
ISSN | 2023 Impact Factor: 2.7 2023 SCImago Journal Rankings: 0.543 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Ge, R | en_HK |
dc.contributor.author | Sun, X | en_HK |
dc.contributor.author | Gu, Q | en_HK |
dc.contributor.author | Watt, RM | en_HK |
dc.contributor.author | Tanner, JA | en_HK |
dc.contributor.author | Wong, BCY | en_HK |
dc.contributor.author | Xia, HH | en_HK |
dc.contributor.author | Huang, JD | en_HK |
dc.contributor.author | He, QY | en_HK |
dc.contributor.author | Sun, H | en_HK |
dc.date.accessioned | 2010-09-06T07:28:07Z | - |
dc.date.available | 2010-09-06T07:28:07Z | - |
dc.date.issued | 2007 | en_HK |
dc.identifier.citation | Journal of Biological Inorganic Chemistry, 2007, v. 12 n. 6, p. 831-842 | en_HK |
dc.identifier.issn | 0949-8257 | en_HK |
dc.identifier.uri | http://hdl.handle.net/10722/77087 | - |
dc.description.abstract | Helicobacter pylori is a major human pathogen that can cause peptic ulcers and chronic gastritis. Bismuth-based triple or quadruple therapies are commonly recommended for the treatment of H. pylori infections. However, the molecular mechanisms underlying treatment with bismuth are currently not fully understood. We have conducted a detailed comparative proteomic analysis of H. pylori cells both before and after treatment with colloidal bismuth subcitrate (CBS). Eight proteins were found to be significantly upregulated or downregulated in the presence of CBS (20 μg mL -1). Bismuth-induced oxidative stress was confirmed by detecting higher levels of lipid hydroperoxide (approximately 1.8 times) and hemin (approximately 3.4 times), in whole cell extracts of bismuth-treated H. pylori cells, compared with those from untreated cells. The presence of bismuth also led to an approximately eightfold decrease in cellular protease activities. Using immobilized-bismuth affinity chromatography, we isolated and subsequently identified seven bismuth-binding proteins from H. pylori cell extracts. The intracellular levels of four of these proteins (HspA, HspB, NapA and TsaA) were influenced by the addition of CBS, which strongly suggests that they interact directly with bismuth. The other bismuth-interacting proteins identified were two enzymes (fumarase and the urease subunit UreB), and a translational factor (Ef-Tu). Our data suggest that the inhibition of proteases, modulation of cellular oxidative stress and interference with nickel homeostasis may be key processes underlying the molecular mechanism of bismuth's actions against H. pylori. © 2007 SBIC. | en_HK |
dc.language | eng | en_HK |
dc.publisher | Springer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00775/index.htm | en_HK |
dc.relation.ispartof | Journal of Biological Inorganic Chemistry | en_HK |
dc.subject | Colloidal bismuth subcitrate | en_HK |
dc.subject | Helicobacter pylori | en_HK |
dc.subject | Immobilized-metal affinity chromatography | en_HK |
dc.subject | Proteomics | en_HK |
dc.subject | Reactive oxygen species | en_HK |
dc.subject.mesh | Bacterial Proteins - drug effects - genetics - metabolism | en_HK |
dc.subject.mesh | Cell Line | en_HK |
dc.subject.mesh | Gene Expression Profiling - methods | en_HK |
dc.subject.mesh | Gene Expression Regulation, Bacterial - drug effects | en_HK |
dc.subject.mesh | Helicobacter pylori - chemistry - drug effects - genetics | en_HK |
dc.subject.mesh | Humans | en_HK |
dc.subject.mesh | Metalloproteins - analysis - genetics | en_HK |
dc.subject.mesh | Microbial Sensitivity Tests | en_HK |
dc.subject.mesh | Nickel - metabolism | en_HK |
dc.subject.mesh | Organometallic Compounds - pharmacology | en_HK |
dc.subject.mesh | Protease Inhibitors | en_HK |
dc.subject.mesh | Proteomics - methods | en_HK |
dc.title | A proteomic approach for the identification of bismuth-binding proteins in Helicobacter pylori | en_HK |
dc.type | Article | en_HK |
dc.identifier.openurl | http://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0949-8257&volume=12&spage=831&epage=42&date=2007&atitle=A+proteomic+approach+for+the+identification+of+bismuth-binding+proteins+in+Helicobacter+pylori | en_HK |
dc.identifier.email | Watt, RM:rmwatt@hku.hk | en_HK |
dc.identifier.email | Tanner, JA:jatanner@hku.hk | en_HK |
dc.identifier.email | Wong, BCY:bcywong@hku.hk | en_HK |
dc.identifier.email | Huang, JD:jdhuang@hkucc.hku.hk | en_HK |
dc.identifier.email | Sun, H:hsun@hkucc.hku.hk | en_HK |
dc.identifier.authority | Watt, RM=rp00043 | en_HK |
dc.identifier.authority | Tanner, JA=rp00495 | en_HK |
dc.identifier.authority | Wong, BCY=rp00429 | en_HK |
dc.identifier.authority | Huang, JD=rp00451 | en_HK |
dc.identifier.authority | Sun, H=rp00777 | en_HK |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1007/s00775-007-0237-7 | en_HK |
dc.identifier.pmid | 17503094 | - |
dc.identifier.scopus | eid_2-s2.0-34547404155 | en_HK |
dc.identifier.hkuros | 131431 | en_HK |
dc.identifier.hkuros | 128580 | - |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-34547404155&selection=ref&src=s&origin=recordpage | en_HK |
dc.identifier.volume | 12 | en_HK |
dc.identifier.issue | 6 | en_HK |
dc.identifier.spage | 831 | en_HK |
dc.identifier.epage | 842 | en_HK |
dc.identifier.isi | WOS:000248414100011 | - |
dc.publisher.place | Germany | en_HK |
dc.identifier.scopusauthorid | Ge, R=7005525090 | en_HK |
dc.identifier.scopusauthorid | Sun, X=8906547400 | en_HK |
dc.identifier.scopusauthorid | Gu, Q=24469982400 | en_HK |
dc.identifier.scopusauthorid | Watt, RM=7102907536 | en_HK |
dc.identifier.scopusauthorid | Tanner, JA=35513993000 | en_HK |
dc.identifier.scopusauthorid | Wong, BCY=7402023340 | en_HK |
dc.identifier.scopusauthorid | Xia, HH=35081494200 | en_HK |
dc.identifier.scopusauthorid | Huang, JD=8108660600 | en_HK |
dc.identifier.scopusauthorid | He, QY=34770287900 | en_HK |
dc.identifier.scopusauthorid | Sun, H=7404827446 | en_HK |
dc.identifier.citeulike | 1681659 | - |
dc.identifier.issnl | 0949-8257 | - |