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Article: The angiogenic effects of Angelica sinensis extract on HUVEC in vitro and zebrafish in vivo

TitleThe angiogenic effects of Angelica sinensis extract on HUVEC in vitro and zebrafish in vivo
Authors
Issue Date2008
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/35503
Citation
Journal Of Cellular Biochemistry, 2008, v. 103 n. 1, p. 195-211 How to Cite?
AbstractAngiogenesis plays an important role in a wide range of physiological processes such as wound healing and fetal development. Many diseases are associated with imbalances in regulation of angiogenesis, in which it is either excessive or there is insufficient blood vessel formation. Angelica sinensis (AS), commonly used in the prescriptions of Chinese medicine, is a potential candidate for curing such diseases. However, biological effects of AS on angiogenesis and underlying mechanisms are yet to be fully elucidated. This investigation describes the angiogenic effects of AS extract on human endothelial cells (HUVEC) in vitro and zebrafish in vivo. The extract was demonstrated, by XTT assay and microscopic cell counting, to stimulate the proliferation of HUVEC; in addition, flow cytometry analysis indicated that the extract increased the percentage of HUVEC in the S phase. The wound healing migration assay illustrated that a dramatic increase in migration could be measured in AS extract-treated HUVEC. Meanwhile, the number of invaded cells and the mean tube length were significantly increased in AS extract treatment groups. The extract was also demonstrated to promote changes in subintestinal vessels (SIVs) in zebrafish, one feature of angiogenesis. In addition, AS extract was found by real-time PCR to enhance vascular endothelial growth factor (VEGF) mRNA expression. In a bead-based immunoassay, higher levels of p38 and JN K1/2 expression were also observed in effusions compared with control cells. All results suggest that Angelica sinensis extract can promote angiogenesis, and that the angiogenic effects involve p38 and JNK 1/2 phosphorylation. © 2007 Wiley-Liss, Inc.
Persistent Identifierhttp://hdl.handle.net/10722/76830
ISSN
2015 Impact Factor: 3.446
2015 SCImago Journal Rankings: 1.520
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLam, HWen_HK
dc.contributor.authorLin, HCen_HK
dc.contributor.authorLao, SCen_HK
dc.contributor.authorGao, JLen_HK
dc.contributor.authorHong, SJen_HK
dc.contributor.authorLeong, CWen_HK
dc.contributor.authorYue, PYKen_HK
dc.contributor.authorKwan, YWen_HK
dc.contributor.authorLeung, AYHen_HK
dc.contributor.authorWang, YTen_HK
dc.contributor.authorLee, SMYen_HK
dc.date.accessioned2010-09-06T07:25:22Z-
dc.date.available2010-09-06T07:25:22Z-
dc.date.issued2008en_HK
dc.identifier.citationJournal Of Cellular Biochemistry, 2008, v. 103 n. 1, p. 195-211en_HK
dc.identifier.issn0730-2312en_HK
dc.identifier.urihttp://hdl.handle.net/10722/76830-
dc.description.abstractAngiogenesis plays an important role in a wide range of physiological processes such as wound healing and fetal development. Many diseases are associated with imbalances in regulation of angiogenesis, in which it is either excessive or there is insufficient blood vessel formation. Angelica sinensis (AS), commonly used in the prescriptions of Chinese medicine, is a potential candidate for curing such diseases. However, biological effects of AS on angiogenesis and underlying mechanisms are yet to be fully elucidated. This investigation describes the angiogenic effects of AS extract on human endothelial cells (HUVEC) in vitro and zebrafish in vivo. The extract was demonstrated, by XTT assay and microscopic cell counting, to stimulate the proliferation of HUVEC; in addition, flow cytometry analysis indicated that the extract increased the percentage of HUVEC in the S phase. The wound healing migration assay illustrated that a dramatic increase in migration could be measured in AS extract-treated HUVEC. Meanwhile, the number of invaded cells and the mean tube length were significantly increased in AS extract treatment groups. The extract was also demonstrated to promote changes in subintestinal vessels (SIVs) in zebrafish, one feature of angiogenesis. In addition, AS extract was found by real-time PCR to enhance vascular endothelial growth factor (VEGF) mRNA expression. In a bead-based immunoassay, higher levels of p38 and JN K1/2 expression were also observed in effusions compared with control cells. All results suggest that Angelica sinensis extract can promote angiogenesis, and that the angiogenic effects involve p38 and JNK 1/2 phosphorylation. © 2007 Wiley-Liss, Inc.en_HK
dc.languageengen_HK
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/35503en_HK
dc.relation.ispartofJournal of Cellular Biochemistryen_HK
dc.rightsJournal of Cellular Biochemistry. Copyright © John Wiley & Sons, Inc.en_HK
dc.subject.meshAngelica sinensis - chemistryen_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshCell Movement - drug effectsen_HK
dc.subject.meshCell Proliferation - drug effectsen_HK
dc.subject.meshCells, Cultureden_HK
dc.subject.meshEndothelial Cells - cytology - drug effects - metabolismen_HK
dc.subject.meshExtracellular Signal-Regulated MAP Kinases - metabolismen_HK
dc.subject.meshGene Expression Regulation - drug effectsen_HK
dc.subject.meshHumansen_HK
dc.subject.meshMAP Kinase Signaling System - drug effectsen_HK
dc.subject.meshNeovascularization, Physiologic - drug effectsen_HK
dc.subject.meshPhenotypeen_HK
dc.subject.meshPlant Extracts - chemistry - pharmacologyen_HK
dc.subject.meshRNA, Messenger - geneticsen_HK
dc.subject.meshS Phase - drug effectsen_HK
dc.subject.meshUmbilical Veins - blood supply - cytology - drug effects - metabolismen_HK
dc.subject.meshZebrafishen_HK
dc.subject.meshp38 Mitogen-Activated Protein Kinases - metabolismen_HK
dc.titleThe angiogenic effects of Angelica sinensis extract on HUVEC in vitro and zebrafish in vivoen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0730-2312&volume=103&issue=1&spage=195 &epage= 211&date=2007&atitle=The+angiogenic+effects+of+Angelica+sinensis+extract+on+HUVEC+in+vitro+and+zebrafish+in+vivo+en_HK
dc.identifier.emailLeung, AYH:ayhleung@hku.hken_HK
dc.identifier.authorityLeung, AYH=rp00265en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1002/jcb.21403en_HK
dc.identifier.pmid17497682-
dc.identifier.scopuseid_2-s2.0-38149013793en_HK
dc.identifier.hkuros157067en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-38149013793&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume103en_HK
dc.identifier.issue1en_HK
dc.identifier.spage195en_HK
dc.identifier.epage211en_HK
dc.identifier.isiWOS:000252334400016-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridLam, HW=23397470200en_HK
dc.identifier.scopusauthoridLin, HC=23397349100en_HK
dc.identifier.scopusauthoridLao, SC=16645820100en_HK
dc.identifier.scopusauthoridGao, JL=23018670200en_HK
dc.identifier.scopusauthoridHong, SJ=23396465100en_HK
dc.identifier.scopusauthoridLeong, CW=16646052800en_HK
dc.identifier.scopusauthoridYue, PYK=8570616200en_HK
dc.identifier.scopusauthoridKwan, YW=7005662153en_HK
dc.identifier.scopusauthoridLeung, AYH=7403012668en_HK
dc.identifier.scopusauthoridWang, YT=8327361900en_HK
dc.identifier.scopusauthoridLee, SMY=35233892600en_HK

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