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Article: Analysis of charge distribution of lambda- and kappa-IgA in IgA nephropathy by focused antigen capture immunoassay

TitleAnalysis of charge distribution of lambda- and kappa-IgA in IgA nephropathy by focused antigen capture immunoassay
Authors
KeywordsFocused antigen capture immunoassay
IgA
IgA nephropathy
Isoelectric focusing
Issue Date1997
PublisherInforma Healthcare. The Journal's web site is located at http://www.tandf.co.uk/journals/titles/00365599.asp
Citation
Scandinavian Journal Of Urology And Nephrology, 1997, v. 31 n. 3, p. 289-293 How to Cite?
AbstractThey finding that eluted mesangial IgA and serum IgA from patients with IgA nephropathy (IgAN) had a restricted anionic charge contrasting with normal serum IgA prompted us to examine the charge of κ- and λ-subclasses of IgA. Previous studies are not totally satisfactory because either total IgA without further dividing into subclass or only κ- and λ-IgA 1 was examined. A new approach is described to study the electrostatic property of total IgA and its light chain subclasses. The new focused antigen capture immunoassay (FACIA) allows us to separate the immunoglobulins by isoelectric point, then to capture by the heavy chain class and finally to visualize according to the light chain class. This method works well with whole blood or serum without the need of prior purification by affinity chromatography. The serum total IgA and λ-IgA levels in patients with IgAN were significantly higher than that of healthy controls. Elevated λ-IgA levels in patients with IgAN resulted in reduced κ/λ ratio of total IgA. Similar to our previous findings in IgA 1 with O-linked oligosaccharide side chain, λ- IgA from patients with IgAN or health controls is highly anionic whereas κ- IgA is relatively cationic. The higher anionic/cationic ratio observed in total IgA from patients compared with controls was due to the higher concentration of λ-IgA from the former group. Raised antonio λ-IgA in IgAN may be contributory to the immumopathogenesis through its selective mesangial binding.
Persistent Identifierhttp://hdl.handle.net/10722/76777
ISSN
2014 Impact Factor: 1.243
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorSuen, KKYen_HK
dc.contributor.authorLewis, WHPen_HK
dc.contributor.authorLai, KNen_HK
dc.date.accessioned2010-09-06T07:24:48Z-
dc.date.available2010-09-06T07:24:48Z-
dc.date.issued1997en_HK
dc.identifier.citationScandinavian Journal Of Urology And Nephrology, 1997, v. 31 n. 3, p. 289-293en_HK
dc.identifier.issn0036-5599en_HK
dc.identifier.urihttp://hdl.handle.net/10722/76777-
dc.description.abstractThey finding that eluted mesangial IgA and serum IgA from patients with IgA nephropathy (IgAN) had a restricted anionic charge contrasting with normal serum IgA prompted us to examine the charge of κ- and λ-subclasses of IgA. Previous studies are not totally satisfactory because either total IgA without further dividing into subclass or only κ- and λ-IgA 1 was examined. A new approach is described to study the electrostatic property of total IgA and its light chain subclasses. The new focused antigen capture immunoassay (FACIA) allows us to separate the immunoglobulins by isoelectric point, then to capture by the heavy chain class and finally to visualize according to the light chain class. This method works well with whole blood or serum without the need of prior purification by affinity chromatography. The serum total IgA and λ-IgA levels in patients with IgAN were significantly higher than that of healthy controls. Elevated λ-IgA levels in patients with IgAN resulted in reduced κ/λ ratio of total IgA. Similar to our previous findings in IgA 1 with O-linked oligosaccharide side chain, λ- IgA from patients with IgAN or health controls is highly anionic whereas κ- IgA is relatively cationic. The higher anionic/cationic ratio observed in total IgA from patients compared with controls was due to the higher concentration of λ-IgA from the former group. Raised antonio λ-IgA in IgAN may be contributory to the immumopathogenesis through its selective mesangial binding.en_HK
dc.languageengen_HK
dc.publisherInforma Healthcare. The Journal's web site is located at http://www.tandf.co.uk/journals/titles/00365599.aspen_HK
dc.relation.ispartofScandinavian Journal of Urology and Nephrologyen_HK
dc.rightsScandinavian Journal of Urology and Nephrology. Copyright © Informa Healthcare.en_HK
dc.subjectFocused antigen capture immunoassayen_HK
dc.subjectIgAen_HK
dc.subjectIgA nephropathyen_HK
dc.subjectIsoelectric focusingen_HK
dc.titleAnalysis of charge distribution of lambda- and kappa-IgA in IgA nephropathy by focused antigen capture immunoassayen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0036-5599&volume=31&spage=289&epage=293&date=1997&atitle=Analysis+of+charge+distribution+of+lambda+-+and+kappa-IgA+in+IgA+nephropathy+by+focused+antigen+capture+immunoassayen_HK
dc.identifier.emailLai, KN: knlai@hku.hken_HK
dc.identifier.authorityLai, KN=rp00324en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.pmid9249895en_HK
dc.identifier.scopuseid_2-s2.0-0030741767en_HK
dc.identifier.hkuros41370en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0030741767&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume31en_HK
dc.identifier.issue3en_HK
dc.identifier.spage289en_HK
dc.identifier.epage293en_HK
dc.identifier.isiWOS:A1997XJ04800012-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridSuen, KKY=8072332500en_HK
dc.identifier.scopusauthoridLewis, WHP=7202045417en_HK
dc.identifier.scopusauthoridLai, KN=7402135706en_HK

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