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Article: Aldehyde dehydrogenase activity in leukemic blasts defines a subgroup of acute myeloid leukemia with adverse prognosis and superior NOD/SCID engrafting potential

TitleAldehyde dehydrogenase activity in leukemic blasts defines a subgroup of acute myeloid leukemia with adverse prognosis and superior NOD/SCID engrafting potential
Authors
Issue Date2007
PublisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/leu
Citation
Leukemia, 2007, v. 21 n. 7, p. 1423-1430 How to Cite?
AbstractAldehyde dehydrogenase (ALDH) activity is used to define normal hematopoietic stem cell (HSC), but its link to leukemic stem cells (LSC) in acute myeloid leukemia (AML) is currently unknown. We hypothesize that ALDH activity in AML might be correlated with the presence of LSC. Fifty-eight bone marrow (BM) samples were collected from AML (n=43), acute lymphoblastic leukemia (ALL) (n=8) and normal cases (n=7). In 14 AML cases, a high SSC loALDH br cell population was identified (ALDH +AML) (median: 14.89%, range: 5.65-48.01%), with the majority of the SSC loALDH br cells coexpressing CD34 +. In another 29 cases, there was undetectable (n=23) or rare (≥ 5%) (n=6) SSC lo ALDH br population (ALDH -AML). Among other clinicopathologic variables, ALDH +AML was significantly associated with adverse cytogenetic abnormalities. CD34 + BM cells from ALDH +AML engrafted significantly better in NOD/ SCID mice (ALDH +AML: injected bone 21.11±9.07%; uninjected bone 1.52±0.75% vs ALDH -AML: injected bone 1.77±1.66% (P=0.05); uninjected bone 0.23±0.23% (P=0.03)) with the engrafting cells showing molecular and cytogenetic aberrations identical to the original clones. Normal BM contained a small SSC loALDH br population (median: 2.92%, range: 0.92-5.79%), but none of the ALL cases showed this fraction. In conclusion, SSC loALDH br cells in ALDH + AML might denote primitive LSC and confer an inferior prognosis in patients.
Persistent Identifierhttp://hdl.handle.net/10722/76707
ISSN
2015 Impact Factor: 12.104
2015 SCImago Journal Rankings: 5.142
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorCheung, AMSen_HK
dc.contributor.authorWan, TSKen_HK
dc.contributor.authorLeung, JCKen_HK
dc.contributor.authorChan, LYYen_HK
dc.contributor.authorHuang, Hen_HK
dc.contributor.authorKwong, YLen_HK
dc.contributor.authorLiang, Ren_HK
dc.contributor.authorLeung, AYHen_HK
dc.date.accessioned2010-09-06T07:24:05Z-
dc.date.available2010-09-06T07:24:05Z-
dc.date.issued2007en_HK
dc.identifier.citationLeukemia, 2007, v. 21 n. 7, p. 1423-1430en_HK
dc.identifier.issn0887-6924en_HK
dc.identifier.urihttp://hdl.handle.net/10722/76707-
dc.description.abstractAldehyde dehydrogenase (ALDH) activity is used to define normal hematopoietic stem cell (HSC), but its link to leukemic stem cells (LSC) in acute myeloid leukemia (AML) is currently unknown. We hypothesize that ALDH activity in AML might be correlated with the presence of LSC. Fifty-eight bone marrow (BM) samples were collected from AML (n=43), acute lymphoblastic leukemia (ALL) (n=8) and normal cases (n=7). In 14 AML cases, a high SSC loALDH br cell population was identified (ALDH +AML) (median: 14.89%, range: 5.65-48.01%), with the majority of the SSC loALDH br cells coexpressing CD34 +. In another 29 cases, there was undetectable (n=23) or rare (≥ 5%) (n=6) SSC lo ALDH br population (ALDH -AML). Among other clinicopathologic variables, ALDH +AML was significantly associated with adverse cytogenetic abnormalities. CD34 + BM cells from ALDH +AML engrafted significantly better in NOD/ SCID mice (ALDH +AML: injected bone 21.11±9.07%; uninjected bone 1.52±0.75% vs ALDH -AML: injected bone 1.77±1.66% (P=0.05); uninjected bone 0.23±0.23% (P=0.03)) with the engrafting cells showing molecular and cytogenetic aberrations identical to the original clones. Normal BM contained a small SSC loALDH br population (median: 2.92%, range: 0.92-5.79%), but none of the ALL cases showed this fraction. In conclusion, SSC loALDH br cells in ALDH + AML might denote primitive LSC and confer an inferior prognosis in patients.en_HK
dc.languageengen_HK
dc.publisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/leuen_HK
dc.relation.ispartofLeukemiaen_HK
dc.subject.meshAldehyde Dehydrogenase - analysis-
dc.subject.meshLeukemia, Myeloid - diagnosis - pathology-
dc.subject.meshNeoplasm Transplantation-
dc.subject.meshNeoplastic Stem Cells - pathology-
dc.titleAldehyde dehydrogenase activity in leukemic blasts defines a subgroup of acute myeloid leukemia with adverse prognosis and superior NOD/SCID engrafting potentialen_HK
dc.typeArticleen_HK
dc.identifier.emailCheung, AMS:h9945256@graduate.hku.hken_HK
dc.identifier.emailLeung, JCK:jckleung@hku.hken_HK
dc.identifier.emailKwong, YL:ylkwong@hku.hken_HK
dc.identifier.emailLiang, R:rliang@hku.hken_HK
dc.identifier.emailLeung, AYH:ayhleung@hku.hken_HK
dc.identifier.authorityCheung, AMS=rp01572en_HK
dc.identifier.authorityLeung, JCK=rp00448en_HK
dc.identifier.authorityKwong, YL=rp00358en_HK
dc.identifier.authorityLiang, R=rp00345en_HK
dc.identifier.authorityLeung, AYH=rp00265en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1038/sj.leu.2404721en_HK
dc.identifier.pmid17476279-
dc.identifier.scopuseid_2-s2.0-34250732230en_HK
dc.identifier.hkuros135393en_HK
dc.identifier.hkuros137479-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-34250732230&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume21en_HK
dc.identifier.issue7en_HK
dc.identifier.spage1423en_HK
dc.identifier.epage1430en_HK
dc.identifier.isiWOS:000247494700010-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridCheung, AMS=36985759800en_HK
dc.identifier.scopusauthoridWan, TSK=25623981600en_HK
dc.identifier.scopusauthoridLeung, JCK=7202180349en_HK
dc.identifier.scopusauthoridChan, LYY=35336076700en_HK
dc.identifier.scopusauthoridHuang, H=7405614998en_HK
dc.identifier.scopusauthoridKwong, YL=7102818954en_HK
dc.identifier.scopusauthoridLiang, R=26643224900en_HK
dc.identifier.scopusauthoridLeung, AYH=7403012668en_HK
dc.identifier.citeulike1275650-

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