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Article: Genomic changes in regenerated porcine coronary arterial endothelial cells

TitleGenomic changes in regenerated porcine coronary arterial endothelial cells
Authors
KeywordsCoagulation
Endothelial regeneration
Extracellular matrix
Genomics
Lipids
Nitric oxide
ROS
Issue Date2007
PublisherLippincott Williams & Wilkins. The Journal's web site is located at http://www.lww.com/product/?1079-5642
Citation
Arteriosclerosis, Thrombosis, And Vascular Biology, 2007, v. 27 n. 11, p. 2443-2449 How to Cite?
Abstract
OBJECTIVE - Genomic changes were defined in cultures of regenerated porcine coronary endothelial cells to explain the alterations that underlie their dysfunction. METHODS AND RESULTS - Regeneration of the endothelium was triggered in vivo by endothelial balloon denudation. After 28 days, both left circumflex (native cells) and left anterior descending (regenerated cells) coronary arteries were dissected, their endothelial cells harvested, and primary cultures established. The basal cyclic GMP production was reduced in regenerated cells without significant reduction in the response to bradykinin and A23187. The mRNA expression levels in both native and regenerated cells were measured by microarray and RT-PCR. The comparison revealed genomic changes related to vasomotor control (cyclooxygenase-1, angiotensin II receptor), coagulation (F2 and TFPI), oxidative stress (Mn SOD, GPX3, and GSR), lipid metabolism (PLA2 and HPGD), and extracellular matrix (MMPs). A-FABP and MMP7 were induced by regeneration. RT-PCR revealed upregulation of A-FABP and downregulation of eNOS and TR. The differential gene expression profiles were confirmed at the protein level by Western blotting for eNOS, F2, Mn SOD, MMP7, and TR. CONCLUSIONS - Cultures from regenerated coronary endothelial cells exhibit genomic changes explaining endothelial dysfunction and suggesting facilitation of coagulation, lipid peroxidation, and extracellular matrix remodeling. © 2007 American Heart Association, Inc.
Persistent Identifierhttp://hdl.handle.net/10722/76692
ISSN
2013 Impact Factor: 5.533
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLee, MYKen_HK
dc.contributor.authorTse, HFen_HK
dc.contributor.authorSiu, CWen_HK
dc.contributor.authorZhu, SGen_HK
dc.contributor.authorMan, RYKen_HK
dc.contributor.authorVanhoutte, PMen_HK
dc.date.accessioned2010-09-06T07:23:55Z-
dc.date.available2010-09-06T07:23:55Z-
dc.date.issued2007en_HK
dc.identifier.citationArteriosclerosis, Thrombosis, And Vascular Biology, 2007, v. 27 n. 11, p. 2443-2449en_HK
dc.identifier.issn1079-5642en_HK
dc.identifier.urihttp://hdl.handle.net/10722/76692-
dc.description.abstractOBJECTIVE - Genomic changes were defined in cultures of regenerated porcine coronary endothelial cells to explain the alterations that underlie their dysfunction. METHODS AND RESULTS - Regeneration of the endothelium was triggered in vivo by endothelial balloon denudation. After 28 days, both left circumflex (native cells) and left anterior descending (regenerated cells) coronary arteries were dissected, their endothelial cells harvested, and primary cultures established. The basal cyclic GMP production was reduced in regenerated cells without significant reduction in the response to bradykinin and A23187. The mRNA expression levels in both native and regenerated cells were measured by microarray and RT-PCR. The comparison revealed genomic changes related to vasomotor control (cyclooxygenase-1, angiotensin II receptor), coagulation (F2 and TFPI), oxidative stress (Mn SOD, GPX3, and GSR), lipid metabolism (PLA2 and HPGD), and extracellular matrix (MMPs). A-FABP and MMP7 were induced by regeneration. RT-PCR revealed upregulation of A-FABP and downregulation of eNOS and TR. The differential gene expression profiles were confirmed at the protein level by Western blotting for eNOS, F2, Mn SOD, MMP7, and TR. CONCLUSIONS - Cultures from regenerated coronary endothelial cells exhibit genomic changes explaining endothelial dysfunction and suggesting facilitation of coagulation, lipid peroxidation, and extracellular matrix remodeling. © 2007 American Heart Association, Inc.en_HK
dc.languageengen_HK
dc.publisherLippincott Williams & Wilkins. The Journal's web site is located at http://www.lww.com/product/?1079-5642en_HK
dc.relation.ispartofArteriosclerosis, Thrombosis, and Vascular Biologyen_HK
dc.rightsArteriosclerosis, Thrombosis, and Vascular Biology. Copyright © Lippincott Williams & Wilkins.en_HK
dc.subjectCoagulationen_HK
dc.subjectEndothelial regenerationen_HK
dc.subjectExtracellular matrixen_HK
dc.subjectGenomicsen_HK
dc.subjectLipidsen_HK
dc.subjectNitric oxideen_HK
dc.subjectROSen_HK
dc.titleGenomic changes in regenerated porcine coronary arterial endothelial cellsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1079-5642&volume=27&spage=2443&epage=2449&date=2007&atitle=Genomic+changes+in+regenerated+porcine+coronary+arterial+endothelial+cells+en_HK
dc.identifier.emailTse, HF: hftse@hkucc.hku.hken_HK
dc.identifier.emailSiu, CW: cwdsiu@hkucc.hku.hken_HK
dc.identifier.emailMan, RYK: rykman@hkucc.hku.hken_HK
dc.identifier.emailVanhoutte, PM: vanhoutt@hku.hken_HK
dc.identifier.authorityTse, HF=rp00428en_HK
dc.identifier.authoritySiu, CW=rp00534en_HK
dc.identifier.authorityMan, RYK=rp00236en_HK
dc.identifier.authorityVanhoutte, PM=rp00238en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1161/ATVBAHA.107.141705en_HK
dc.identifier.scopuseid_2-s2.0-36048970965en_HK
dc.identifier.hkuros147765en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-36048970965&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume27en_HK
dc.identifier.issue11en_HK
dc.identifier.spage2443en_HK
dc.identifier.epage2449en_HK
dc.identifier.isiWOS:000250424700025-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridLee, MYK=22980015700en_HK
dc.identifier.scopusauthoridTse, HF=7006070805en_HK
dc.identifier.scopusauthoridSiu, CW=7006550690en_HK
dc.identifier.scopusauthoridZhu, SG=55237337600en_HK
dc.identifier.scopusauthoridMan, RYK=7004986435en_HK
dc.identifier.scopusauthoridVanhoutte, PM=7202304247en_HK

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