Article: Genomic changes in regenerated porcine coronary arterial endothelial cells

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TitleGenomic changes in regenerated porcine coronary arterial endothelial cells
AuthorsLee, MYK1
Tse, HF1
Siu, CW1
Zhu, SG1
Man, RYK1
Vanhoutte, PM1
KeywordsCoagulation
Endothelial regeneration
Extracellular matrix
Genomics
Lipids
Nitric oxide
ROS
Issue Date2007
PublisherLippincott Williams & Wilkins. The Journal's web site is located at http://www.lww.com/product/?1079-5642
CitationArteriosclerosis, Thrombosis, And Vascular Biology, 2007, v. 27 n. 11, p. 2443-2449 [How to Cite?]
DOI: http://dx.doi.org/10.1161/ATVBAHA.107.141705
AbstractOBJECTIVE - Genomic changes were defined in cultures of regenerated porcine coronary endothelial cells to explain the alterations that underlie their dysfunction. METHODS AND RESULTS - Regeneration of the endothelium was triggered in vivo by endothelial balloon denudation. After 28 days, both left circumflex (native cells) and left anterior descending (regenerated cells) coronary arteries were dissected, their endothelial cells harvested, and primary cultures established. The basal cyclic GMP production was reduced in regenerated cells without significant reduction in the response to bradykinin and A23187. The mRNA expression levels in both native and regenerated cells were measured by microarray and RT-PCR. The comparison revealed genomic changes related to vasomotor control (cyclooxygenase-1, angiotensin II receptor), coagulation (F2 and TFPI), oxidative stress (Mn SOD, GPX3, and GSR), lipid metabolism (PLA2 and HPGD), and extracellular matrix (MMPs). A-FABP and MMP7 were induced by regeneration. RT-PCR revealed upregulation of A-FABP and downregulation of eNOS and TR. The differential gene expression profiles were confirmed at the protein level by Western blotting for eNOS, F2, Mn SOD, MMP7, and TR. CONCLUSIONS - Cultures from regenerated coronary endothelial cells exhibit genomic changes explaining endothelial dysfunction and suggesting facilitation of coagulation, lipid peroxidation, and extracellular matrix remodeling. © 2007 American Heart Association, Inc.
ISSN1079-5642
2011 Impact Factor: 6.368
2011 SCImago Journal Rankings: 0.891
DOIhttp://dx.doi.org/10.1161/ATVBAHA.107.141705
ReferencesReferences in Scopus
DC Field
Value
dc.contributor.authorLee, MYK
dc.contributor.authorTse, HF
dc.contributor.authorSiu, CW
dc.contributor.authorZhu, SG
dc.contributor.authorMan, RYK
dc.contributor.authorVanhoutte, PM
dc.date.accessioned2010-09-06T07:23:55Z
dc.date.available2010-09-06T07:23:55Z
dc.date.issued2007
dc.description.abstractOBJECTIVE - Genomic changes were defined in cultures of regenerated porcine coronary endothelial cells to explain the alterations that underlie their dysfunction. METHODS AND RESULTS - Regeneration of the endothelium was triggered in vivo by endothelial balloon denudation. After 28 days, both left circumflex (native cells) and left anterior descending (regenerated cells) coronary arteries were dissected, their endothelial cells harvested, and primary cultures established. The basal cyclic GMP production was reduced in regenerated cells without significant reduction in the response to bradykinin and A23187. The mRNA expression levels in both native and regenerated cells were measured by microarray and RT-PCR. The comparison revealed genomic changes related to vasomotor control (cyclooxygenase-1, angiotensin II receptor), coagulation (F2 and TFPI), oxidative stress (Mn SOD, GPX3, and GSR), lipid metabolism (PLA2 and HPGD), and extracellular matrix (MMPs). A-FABP and MMP7 were induced by regeneration. RT-PCR revealed upregulation of A-FABP and downregulation of eNOS and TR. The differential gene expression profiles were confirmed at the protein level by Western blotting for eNOS, F2, Mn SOD, MMP7, and TR. CONCLUSIONS - Cultures from regenerated coronary endothelial cells exhibit genomic changes explaining endothelial dysfunction and suggesting facilitation of coagulation, lipid peroxidation, and extracellular matrix remodeling. © 2007 American Heart Association, Inc.
dc.description.natureLink_to_subscribed_fulltext
dc.identifier.citationArteriosclerosis, Thrombosis, And Vascular Biology, 2007, v. 27 n. 11, p. 2443-2449 [How to Cite?]
DOI: http://dx.doi.org/10.1161/ATVBAHA.107.141705
dc.identifier.doihttp://dx.doi.org/10.1161/ATVBAHA.107.141705
dc.identifier.epage2449
dc.identifier.hkuros147765
dc.identifier.isiWOS:000250424700025
dc.identifier.issn1079-5642
2011 Impact Factor: 6.368
2011 SCImago Journal Rankings: 0.891
dc.identifier.issue11
dc.identifier.openurl
dc.identifier.scopuseid_2-s2.0-36048970965
dc.identifier.spage2443
dc.identifier.urihttp://hdl.handle.net/10722/76692
dc.identifier.volume27
dc.languageeng
dc.publisherLippincott Williams & Wilkins. The Journal's web site is located at http://www.lww.com/product/?1079-5642
dc.publisher.placeUnited States
dc.relation.ispartofArteriosclerosis, Thrombosis, and Vascular Biology
dc.relation.referencesReferences in Scopus
dc.rightsArteriosclerosis, Thrombosis, and Vascular Biology. Copyright © Lippincott Williams & Wilkins.
dc.subjectCoagulation
dc.subjectEndothelial regeneration
dc.subjectExtracellular matrix
dc.subjectGenomics
dc.subjectLipids
dc.subjectNitric oxide
dc.subjectROS
dc.titleGenomic changes in regenerated porcine coronary arterial endothelial cells
dc.typeArticle
Author Affiliations
  1. The University of Hong Kong Li Ka Shing Faculty of Medicine