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Article: Increased ascitic level of hyaluronan in liver cirrhosis

TitleIncreased ascitic level of hyaluronan in liver cirrhosis
Authors
Issue Date1998
PublisherMosby, Inc. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/709553/description#description
Citation
Journal Of Laboratory And Clinical Medicine, 1998, v. 131 n. 4, p. 354-359 How to Cite?
AbstractHyaluronan (HA) is a polysaccharide that forms a critical component of extracellular matrixes. It is present in high concentrations in tissues undergoing remodeling and morphogenesis. Serum HA is elevated in patients with chronic liver disease, and this has been considered to be caused by impaired degradation by the liver endothelial cells. We studied the level of HA In the ascitic fluid and plasma from 27 patients with cirrhotic ascites. These values were compared with peritoneal dialysate effluent (PDE) and plasma from 33 patients with uremia who were undergoing continuous ambulatory peritoneal dialysis (CAPD). The median HA levels in ascitic fluid and plasma from our 26 patients with cirrhosis were significantly higher than corresponding PDE and plasma values from the 33 CAPD patients (p < 0.0001). The median peritoneal/plasma ratios of creatinine, albumin, and immunoglobulin G in either cirrhotic or CAPD patients were less than unity. In contrast, the median peritoneal/plasma ratios of HA in both groups of patients exceeded one with a higher peritoneal/plasma ratio of HA in patients with cirrhosis (p = 0.0035). A significant correlation was observed between the ascitic level of HA and interleukin-1β, interleukln-6, or transforming growth factor-β. Our in vitro cell culture studies revealed that HA is synthesized by both mesothelial cells and macrophages. We observed an additive effect in the synthesis of HA by mesothe-lial cells when the macrophage-conditioned medium was added to the RPMI culture medium. We conclude that a high level of HA is found In ascites from patients with cirrhosis. Our results strongly suggest that simultaneous Increased synthesis of HA by the peritoneal cells and a reduction of degradation by liver endothelial cells occur in these patients with cirrhosis with ascites. This event of Increased HA synthesis may be contributory to remodeling and regeneration of the peritoneal lining.
Persistent Identifierhttp://hdl.handle.net/10722/76574
ISSN
2008 Impact Factor: 2.795
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLai, KNen_HK
dc.contributor.authorSzeto, CCen_HK
dc.contributor.authorLam, CWKen_HK
dc.contributor.authorLai, KBen_HK
dc.contributor.authorWong, TYHen_HK
dc.contributor.authorLeung, JCKen_HK
dc.date.accessioned2010-09-06T07:22:41Z-
dc.date.available2010-09-06T07:22:41Z-
dc.date.issued1998en_HK
dc.identifier.citationJournal Of Laboratory And Clinical Medicine, 1998, v. 131 n. 4, p. 354-359en_HK
dc.identifier.issn0022-2143en_HK
dc.identifier.urihttp://hdl.handle.net/10722/76574-
dc.description.abstractHyaluronan (HA) is a polysaccharide that forms a critical component of extracellular matrixes. It is present in high concentrations in tissues undergoing remodeling and morphogenesis. Serum HA is elevated in patients with chronic liver disease, and this has been considered to be caused by impaired degradation by the liver endothelial cells. We studied the level of HA In the ascitic fluid and plasma from 27 patients with cirrhotic ascites. These values were compared with peritoneal dialysate effluent (PDE) and plasma from 33 patients with uremia who were undergoing continuous ambulatory peritoneal dialysis (CAPD). The median HA levels in ascitic fluid and plasma from our 26 patients with cirrhosis were significantly higher than corresponding PDE and plasma values from the 33 CAPD patients (p < 0.0001). The median peritoneal/plasma ratios of creatinine, albumin, and immunoglobulin G in either cirrhotic or CAPD patients were less than unity. In contrast, the median peritoneal/plasma ratios of HA in both groups of patients exceeded one with a higher peritoneal/plasma ratio of HA in patients with cirrhosis (p = 0.0035). A significant correlation was observed between the ascitic level of HA and interleukin-1β, interleukln-6, or transforming growth factor-β. Our in vitro cell culture studies revealed that HA is synthesized by both mesothelial cells and macrophages. We observed an additive effect in the synthesis of HA by mesothe-lial cells when the macrophage-conditioned medium was added to the RPMI culture medium. We conclude that a high level of HA is found In ascites from patients with cirrhosis. Our results strongly suggest that simultaneous Increased synthesis of HA by the peritoneal cells and a reduction of degradation by liver endothelial cells occur in these patients with cirrhosis with ascites. This event of Increased HA synthesis may be contributory to remodeling and regeneration of the peritoneal lining.en_HK
dc.languageengen_HK
dc.publisherMosby, Inc. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/709553/description#descriptionen_HK
dc.relation.ispartofJournal of Laboratory and Clinical Medicineen_HK
dc.rightsJournal of Laboratory and Clinical Medicine. Copyright © Mosby, Inc.en_HK
dc.subject.meshAdulten_HK
dc.subject.meshAgeden_HK
dc.subject.meshAscites - metabolismen_HK
dc.subject.meshAscitic Fluid - metabolismen_HK
dc.subject.meshCells, Cultureden_HK
dc.subject.meshEpithelial Cells - metabolismen_HK
dc.subject.meshFemaleen_HK
dc.subject.meshHumansen_HK
dc.subject.meshHyaluronic Acid - blood - metabolismen_HK
dc.subject.meshLiver Cirrhosis - metabolismen_HK
dc.subject.meshMacrophages - metabolismen_HK
dc.subject.meshMaleen_HK
dc.subject.meshMiddle Ageden_HK
dc.titleIncreased ascitic level of hyaluronan in liver cirrhosisen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0022-2143&volume=121&spage=354&epage=359&date=1998&atitle=Increased+ascitic+level+of+hyaluronan+in+liver+cirrhosisen_HK
dc.identifier.emailLai, KN: knlai@hku.hken_HK
dc.identifier.emailLeung, JCK: jckleung@hku.hken_HK
dc.identifier.authorityLai, KN=rp00324en_HK
dc.identifier.authorityLeung, JCK=rp00448en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.pmid9579389-
dc.identifier.scopuseid_2-s2.0-0006722780en_HK
dc.identifier.hkuros41508en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0006722780&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume131en_HK
dc.identifier.issue4en_HK
dc.identifier.spage354en_HK
dc.identifier.epage359en_HK
dc.identifier.isiWOS:000073339300012-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridLai, KN=7402135706en_HK
dc.identifier.scopusauthoridSzeto, CC=35495407200en_HK
dc.identifier.scopusauthoridLam, CWK=7402527629en_HK
dc.identifier.scopusauthoridLai, KB=7402135525en_HK
dc.identifier.scopusauthoridWong, TYH=7403531489en_HK
dc.identifier.scopusauthoridLeung, JCK=7202180349en_HK

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