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Article: Biphasic effects of cell volume on excitation-contraction coupling in rabbit ventricular myocytes

TitleBiphasic effects of cell volume on excitation-contraction coupling in rabbit ventricular myocytes
Authors
Issue Date2002
PublisherAmerican Physiological Society. The Journal's web site is located at http://intl-ajpheart.physiology.org/
Citation
American Journal Of Physiology - Heart And Circulatory Physiology, 2002, v. 282 n. 4 51-4, p. H1270-H1277 How to Cite?
AbstractWe studied the effects of osmotic swelling on the components of excitation-contraction coupling in ventricular myocytes. Myocyte volume rapidly increased 30% in hyposmotic (0.6T) solution and was constant thereafter. Cell shortening transiently increased 31% after 4 min in 0.6T but then decreased to 68% of control after 20 min. In parallel, the L-type Ca2+ current (ICa-L) transiently increased 10% and then declined to 70% of control. Similar biphasic effects on shortening were observed under current clamp. In contrast, action potential duration was unchanged at 4 min but decreased to 72% of control after 20 min. Ca2+ transients were measured with fura 2-AM. The emission ratio with excitation at 340 and 380 nm (f340/f380) decreased by 12% after 3 min in 0.6T, whereas shortening and ICa-L increased at the same time. After 8 min, shortening, ICa-L, and the f340/f380 ratio decreased 28, 25, and 59%, respectively. The results suggest that osmotic swelling causes biphasic changes in ICa-L that contribute to its biphasic effects on contraction. In addition, swelling initially appears to reduce the Ca2+ transient initiated by a given ICa-L, and later, both ICa-L and the Ca2+ transient are inhibited.
Persistent Identifierhttp://hdl.handle.net/10722/76571
ISSN
2015 Impact Factor: 3.324
2015 SCImago Journal Rankings: 1.823
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLi, GRen_HK
dc.contributor.authorZhang, Men_HK
dc.contributor.authorSatin, LSen_HK
dc.contributor.authorBaumgarten, CMen_HK
dc.date.accessioned2010-09-06T07:22:39Z-
dc.date.available2010-09-06T07:22:39Z-
dc.date.issued2002en_HK
dc.identifier.citationAmerican Journal Of Physiology - Heart And Circulatory Physiology, 2002, v. 282 n. 4 51-4, p. H1270-H1277en_HK
dc.identifier.issn0363-6135en_HK
dc.identifier.urihttp://hdl.handle.net/10722/76571-
dc.description.abstractWe studied the effects of osmotic swelling on the components of excitation-contraction coupling in ventricular myocytes. Myocyte volume rapidly increased 30% in hyposmotic (0.6T) solution and was constant thereafter. Cell shortening transiently increased 31% after 4 min in 0.6T but then decreased to 68% of control after 20 min. In parallel, the L-type Ca2+ current (ICa-L) transiently increased 10% and then declined to 70% of control. Similar biphasic effects on shortening were observed under current clamp. In contrast, action potential duration was unchanged at 4 min but decreased to 72% of control after 20 min. Ca2+ transients were measured with fura 2-AM. The emission ratio with excitation at 340 and 380 nm (f340/f380) decreased by 12% after 3 min in 0.6T, whereas shortening and ICa-L increased at the same time. After 8 min, shortening, ICa-L, and the f340/f380 ratio decreased 28, 25, and 59%, respectively. The results suggest that osmotic swelling causes biphasic changes in ICa-L that contribute to its biphasic effects on contraction. In addition, swelling initially appears to reduce the Ca2+ transient initiated by a given ICa-L, and later, both ICa-L and the Ca2+ transient are inhibited.en_HK
dc.languageengen_HK
dc.publisherAmerican Physiological Society. The Journal's web site is located at http://intl-ajpheart.physiology.org/en_HK
dc.relation.ispartofAmerican Journal of Physiology - Heart and Circulatory Physiologyen_HK
dc.subject.meshAction Potentials - physiologyen_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshCalcium Channels, L-Type - physiologyen_HK
dc.subject.meshCell Size - physiologyen_HK
dc.subject.meshHeart - physiologyen_HK
dc.subject.meshHeart Ventriclesen_HK
dc.subject.meshKineticsen_HK
dc.subject.meshMyocardial Contraction - physiologyen_HK
dc.subject.meshMyocardium - cytologyen_HK
dc.subject.meshOsmolar Concentrationen_HK
dc.subject.meshPatch-Clamp Techniquesen_HK
dc.subject.meshRabbitsen_HK
dc.subject.meshTime Factorsen_HK
dc.titleBiphasic effects of cell volume on excitation-contraction coupling in rabbit ventricular myocytesen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0002-9513&volume=282&spage=H1270&epage=H1277&date=2002&atitle=Biphasic+effects+of+cell+volume+on+excitation-contraction+coupling+in+rabbit+ventricular+myocytesen_HK
dc.identifier.emailLi, GR:grli@hkucc.hku.hken_HK
dc.identifier.authorityLi, GR=rp00476en_HK
dc.description.naturelink_to_OA_fulltext-
dc.identifier.pmid11893561-
dc.identifier.scopuseid_2-s2.0-0036081432en_HK
dc.identifier.hkuros112671en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0036081432&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume282en_HK
dc.identifier.issue4 51-4en_HK
dc.identifier.spageH1270en_HK
dc.identifier.epageH1277en_HK
dc.identifier.isiWOS:000174365800013-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridLi, GR=7408462932en_HK
dc.identifier.scopusauthoridZhang, M=36043218200en_HK
dc.identifier.scopusauthoridSatin, LS=7003323653en_HK
dc.identifier.scopusauthoridBaumgarten, CM=7006283434en_HK

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