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Article: Identification and characterization of BCL6 translocation partner genes in primary gastric high-grade B-cell lymphoma: Heat shock protein 89 alpha is a novel fusion partner gene of BCL6

TitleIdentification and characterization of BCL6 translocation partner genes in primary gastric high-grade B-cell lymphoma: Heat shock protein 89 alpha is a novel fusion partner gene of BCL6
Authors
Issue Date2000
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/38250
Citation
Genes Chromosomes And Cancer, 2000, v. 27 n. 1, p. 69-75 How to Cite?
AbstractPrimary gastric high-grade B-cell lymphoma (HGBL) is a special type of non-Hodgkin's lymphoma. So far, the genetic features of this tumor have not been well characterized. Recently, a high incidence of BCL6 rearrangements has been detected in HGBL. However, no previous cytogenetic studies have found translocations involving the BCL6 locus (3q27) in HGBL, and the genetic basis underlying the BCL6 rearrangements in this tumor remains unclear. We therefore characterized the partner genes of BCL6 in five primary gastric HGBLs with a rearranged BCL6 gene by analyzing BCL6 transcripts using the 5' RACE (rapid amplification of cDNA end) strategy. BCL6 translocation partner genes were identified at the 5' end of the chimeric transcripts in all five cases, including the immunoglobulin heavy-chain (IGH) gene in three cases and the immunoglobulin λ-light-chain gene and the heat shock protein 89 alpha (HSP89A) gene in the other two cases. The chimeric transcripts in all cases contained the intact BCL6 exon 2, but lacked exon 1, which was replaced by sequences from the partner genes, suggesting that BCL6 expression was under the control of regulatory sequences of the partner genes. These results, for the first time, indicate that immunoglobulin genes, especially IGH, are the most common BCL6 translocation partner genes in primary gastric HGBL and that HSP89A is a novel partner of BCL6. Because immunoglobulin genes are also the most frequent partners of BCL6 in nodal diffuse large B-cell lymphoma (DLBCL), these data suggest that primary gastric HGBL shares a common genetic basis with nodal DLBCL.
Persistent Identifierhttp://hdl.handle.net/10722/76509
ISSN
2015 Impact Factor: 3.96
2015 SCImago Journal Rankings: 2.210
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorXu, WSen_HK
dc.contributor.authorLiang, RHSen_HK
dc.contributor.authorSrivastava, Gen_HK
dc.date.accessioned2010-09-06T07:22:00Z-
dc.date.available2010-09-06T07:22:00Z-
dc.date.issued2000en_HK
dc.identifier.citationGenes Chromosomes And Cancer, 2000, v. 27 n. 1, p. 69-75en_HK
dc.identifier.issn1045-2257en_HK
dc.identifier.urihttp://hdl.handle.net/10722/76509-
dc.description.abstractPrimary gastric high-grade B-cell lymphoma (HGBL) is a special type of non-Hodgkin's lymphoma. So far, the genetic features of this tumor have not been well characterized. Recently, a high incidence of BCL6 rearrangements has been detected in HGBL. However, no previous cytogenetic studies have found translocations involving the BCL6 locus (3q27) in HGBL, and the genetic basis underlying the BCL6 rearrangements in this tumor remains unclear. We therefore characterized the partner genes of BCL6 in five primary gastric HGBLs with a rearranged BCL6 gene by analyzing BCL6 transcripts using the 5' RACE (rapid amplification of cDNA end) strategy. BCL6 translocation partner genes were identified at the 5' end of the chimeric transcripts in all five cases, including the immunoglobulin heavy-chain (IGH) gene in three cases and the immunoglobulin λ-light-chain gene and the heat shock protein 89 alpha (HSP89A) gene in the other two cases. The chimeric transcripts in all cases contained the intact BCL6 exon 2, but lacked exon 1, which was replaced by sequences from the partner genes, suggesting that BCL6 expression was under the control of regulatory sequences of the partner genes. These results, for the first time, indicate that immunoglobulin genes, especially IGH, are the most common BCL6 translocation partner genes in primary gastric HGBL and that HSP89A is a novel partner of BCL6. Because immunoglobulin genes are also the most frequent partners of BCL6 in nodal diffuse large B-cell lymphoma (DLBCL), these data suggest that primary gastric HGBL shares a common genetic basis with nodal DLBCL.en_HK
dc.languageengen_HK
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/38250en_HK
dc.relation.ispartofGenes Chromosomes and Canceren_HK
dc.rightsGenes, Chromosomes & Cancer. Copyright © John Wiley & Sons, Inc.en_HK
dc.subject.mesh5' Untranslated Regionsen_HK
dc.subject.meshChromosome Mappingen_HK
dc.subject.meshChromosomes, Human, Pair 3 - geneticsen_HK
dc.subject.meshDNA, Complementary - analysisen_HK
dc.subject.meshDNA, Neoplasmen_HK
dc.subject.meshDNA-Binding Proteins - geneticsen_HK
dc.subject.meshGenes, Immunoglobulinen_HK
dc.subject.meshHSP90 Heat-Shock Proteins - chemistry - geneticsen_HK
dc.subject.meshHumansen_HK
dc.subject.meshImmunoglobulin Heavy Chains - geneticsen_HK
dc.subject.meshImmunoglobulin Light Chains - geneticsen_HK
dc.subject.meshLymphoma, B-Cell - geneticsen_HK
dc.subject.meshLymphoma, Non-Hodgkin - geneticsen_HK
dc.subject.meshProto-Oncogene Proteins - geneticsen_HK
dc.subject.meshProto-Oncogene Proteins c-bcl-6en_HK
dc.subject.meshRNA, Messenger - geneticsen_HK
dc.subject.meshRecombinant Fusion Proteins - geneticsen_HK
dc.subject.meshStomach Neoplasms - geneticsen_HK
dc.subject.meshTranscription Factors - geneticsen_HK
dc.subject.meshTranslocation, Genetic - geneticsen_HK
dc.titleIdentification and characterization of BCL6 translocation partner genes in primary gastric high-grade B-cell lymphoma: Heat shock protein 89 alpha is a novel fusion partner gene of BCL6en_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1045-2257&volume=27&spage=69&epage=75&date=2000&atitle=Identification+and+characterization+of+BCL6+translocation+partner+genes+in+primary+gastric+high-grade+B-cell+lymphoma:+heat+shock+protein+89+alpha+is+a+novel+fusion+partner+gene+of+BCL6en_HK
dc.identifier.emailLiang, RHS:rliang@hku.hken_HK
dc.identifier.emailSrivastava, G:gopesh@pathology.hku.hken_HK
dc.identifier.authorityLiang, RHS=rp00345en_HK
dc.identifier.authoritySrivastava, G=rp00365en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1002/(SICI)1098-2264(200001)27:1<69::AID-GCC9>3.0.CO;2-5en_HK
dc.identifier.pmid10564588-
dc.identifier.scopuseid_2-s2.0-0008210433en_HK
dc.identifier.hkuros52632en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0008210433&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume27en_HK
dc.identifier.issue1en_HK
dc.identifier.spage69en_HK
dc.identifier.epage75en_HK
dc.identifier.isiWOS:000083959800009-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridXu, WS=36804007100en_HK
dc.identifier.scopusauthoridLiang, RHS=26643224900en_HK
dc.identifier.scopusauthoridSrivastava, G=7202242238en_HK

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