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Article: Ethanolic extract of Actaea racemosa (black cohosh) potentiates bone nodule formation in MC3T3-E1 preosteoblast cells

TitleEthanolic extract of Actaea racemosa (black cohosh) potentiates bone nodule formation in MC3T3-E1 preosteoblast cells
Authors
Issue Date2008
PublisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/bone
Citation
Bone, 2008, v. 43 n. 3, p. 567-573 How to Cite?
AbstractAceaea racemosa (formerly Cimicifuga racemosa, black cohosh, AR) extracts have been widely used as an alternative to hormonal replacement therapy for menopausal symptoms. Recent evidences suggest AR extracts are also effective in protecting against postmenopausal bone loss. To determine whether AR has any direct anabolic effect on osteoblasts, we investigated the ethanolic extract of AR on bone nodule formation in mouse MC3T3-E1 preosteoblast cells. AR did not stimulate osteoblast proliferation. Rather, at high doses of 1000 ng/mL for 48 h, AR suppressed (7.2 ± 0.9% vs. control) osteoblast proliferation. At 500 ng/mL, a significant increase in bone nodule formation was seen with Von Kossa staining. Using quantitative PCR analysis, AR was shown to enhance the gene expression of runx2 and osteocalcin. Co-treatment with ICI 182,780, the selective estrogen receptor antagonist, abolished the stimulatory effect of AR on runx2 and osteocalcin gene induction, as well as on bone nodule formation in MC3T3-E1 cells. This is a first report of the direct effect of AR on enhancement of bone nodule formation in osteoblasts, and this action was mediated via an estrogen receptor-dependent mechanism. The results provide a scientific rationale at the molecular level for the claim that AR can offer effective prevention of postmenopausal bone loss. © 2008 Elsevier Inc.
Persistent Identifierhttp://hdl.handle.net/10722/76443
ISSN
2015 Impact Factor: 3.736
2015 SCImago Journal Rankings: 1.752
ISI Accession Number ID
Funding AgencyGrant Number
HKU Foundation Bone Health Fund CRCG
Osteoporosis Research Fund of The University of Hong Kong
NIH National Center for Complementary and Alternative MedicineR21 AT01957
Funding Information:

This study is supported by HKU Foundation Bone Health Fund CRCG grant and Osteoporosis Research Fund of The University of Hong Kong. We would like to thank NIH National Center for Complementary and Alternative Medicine grant (R21 AT01957) for the Support of F Kronenbergg, EJ Kennelly and B Jiang.

References

 

DC FieldValueLanguage
dc.contributor.authorChan, BYen_HK
dc.contributor.authorLau, KSen_HK
dc.contributor.authorJiang, Ben_HK
dc.contributor.authorKennelly, EJen_HK
dc.contributor.authorKronenberg, Fen_HK
dc.contributor.authorKung, AWCen_HK
dc.date.accessioned2010-09-06T07:21:17Z-
dc.date.available2010-09-06T07:21:17Z-
dc.date.issued2008en_HK
dc.identifier.citationBone, 2008, v. 43 n. 3, p. 567-573en_HK
dc.identifier.issn8756-3282en_HK
dc.identifier.urihttp://hdl.handle.net/10722/76443-
dc.description.abstractAceaea racemosa (formerly Cimicifuga racemosa, black cohosh, AR) extracts have been widely used as an alternative to hormonal replacement therapy for menopausal symptoms. Recent evidences suggest AR extracts are also effective in protecting against postmenopausal bone loss. To determine whether AR has any direct anabolic effect on osteoblasts, we investigated the ethanolic extract of AR on bone nodule formation in mouse MC3T3-E1 preosteoblast cells. AR did not stimulate osteoblast proliferation. Rather, at high doses of 1000 ng/mL for 48 h, AR suppressed (7.2 ± 0.9% vs. control) osteoblast proliferation. At 500 ng/mL, a significant increase in bone nodule formation was seen with Von Kossa staining. Using quantitative PCR analysis, AR was shown to enhance the gene expression of runx2 and osteocalcin. Co-treatment with ICI 182,780, the selective estrogen receptor antagonist, abolished the stimulatory effect of AR on runx2 and osteocalcin gene induction, as well as on bone nodule formation in MC3T3-E1 cells. This is a first report of the direct effect of AR on enhancement of bone nodule formation in osteoblasts, and this action was mediated via an estrogen receptor-dependent mechanism. The results provide a scientific rationale at the molecular level for the claim that AR can offer effective prevention of postmenopausal bone loss. © 2008 Elsevier Inc.en_HK
dc.languageengen_HK
dc.publisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/boneen_HK
dc.relation.ispartofBoneen_HK
dc.rightsBone. Copyright © Elsevier Inc.en_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshBone and Bones - metabolismen_HK
dc.subject.meshCell Proliferationen_HK
dc.subject.meshCimicifuga - metabolismen_HK
dc.subject.meshCore Binding Factor Alpha 1 Subunit - biosynthesisen_HK
dc.subject.meshDose-Response Relationship, Drugen_HK
dc.subject.meshEstradiol - analogs & derivatives - pharmacologyen_HK
dc.subject.meshEstrogen Antagonists - pharmacologyen_HK
dc.subject.meshEthanol - pharmacologyen_HK
dc.subject.meshMiceen_HK
dc.subject.meshOsteoblasts - cytology - metabolismen_HK
dc.subject.meshOsteocalcin - biosynthesisen_HK
dc.subject.meshOsteogenesis - drug effectsen_HK
dc.subject.meshPlant Extracts - pharmacologyen_HK
dc.subject.meshReceptors, Androgen - metabolismen_HK
dc.titleEthanolic extract of Actaea racemosa (black cohosh) potentiates bone nodule formation in MC3T3-E1 preosteoblast cellsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=8756-3282&volume=43&issue=3&spage=567&epage=73&date=2008&atitle=Ethanolic+extract+of+Actaea+racemosa+(black+cohosh)+potentiates+bone+nodule+formation+in+MC3T3-E1+preosteoblast+cellsen_HK
dc.identifier.emailKung, AWC:awckung@hku.hken_HK
dc.identifier.authorityKung, AWC=rp00368en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.bone.2008.04.018en_HK
dc.identifier.pmid18555764-
dc.identifier.scopuseid_2-s2.0-49149112633en_HK
dc.identifier.hkuros146151en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-49149112633&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume43en_HK
dc.identifier.issue3en_HK
dc.identifier.spage567en_HK
dc.identifier.epage573en_HK
dc.identifier.isiWOS:000258953800021-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridChan, BY=36765897200en_HK
dc.identifier.scopusauthoridLau, KS=35205833900en_HK
dc.identifier.scopusauthoridJiang, B=7402264735en_HK
dc.identifier.scopusauthoridKennelly, EJ=7004641877en_HK
dc.identifier.scopusauthoridKronenberg, F=7006982296en_HK
dc.identifier.scopusauthoridKung, AWC=7102322339en_HK
dc.identifier.citeulike2989744-

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