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Article: Ethanolic extract of Actaea racemosa (black cohosh) potentiates bone nodule formation in MC3T3-E1 preosteoblast cells
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TitleEthanolic extract of Actaea racemosa (black cohosh) potentiates bone nodule formation in MC3T3-E1 preosteoblast cells
 
AuthorsChan, BY2
Lau, KS2
Jiang, B1 3
Kennelly, EJ1
Kronenberg, F3
Kung, AWC2
 
Issue Date2008
 
PublisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/bone
 
CitationBone, 2008, v. 43 n. 3, p. 567-573 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.bone.2008.04.018
 
AbstractAceaea racemosa (formerly Cimicifuga racemosa, black cohosh, AR) extracts have been widely used as an alternative to hormonal replacement therapy for menopausal symptoms. Recent evidences suggest AR extracts are also effective in protecting against postmenopausal bone loss. To determine whether AR has any direct anabolic effect on osteoblasts, we investigated the ethanolic extract of AR on bone nodule formation in mouse MC3T3-E1 preosteoblast cells. AR did not stimulate osteoblast proliferation. Rather, at high doses of 1000 ng/mL for 48 h, AR suppressed (7.2 ± 0.9% vs. control) osteoblast proliferation. At 500 ng/mL, a significant increase in bone nodule formation was seen with Von Kossa staining. Using quantitative PCR analysis, AR was shown to enhance the gene expression of runx2 and osteocalcin. Co-treatment with ICI 182,780, the selective estrogen receptor antagonist, abolished the stimulatory effect of AR on runx2 and osteocalcin gene induction, as well as on bone nodule formation in MC3T3-E1 cells. This is a first report of the direct effect of AR on enhancement of bone nodule formation in osteoblasts, and this action was mediated via an estrogen receptor-dependent mechanism. The results provide a scientific rationale at the molecular level for the claim that AR can offer effective prevention of postmenopausal bone loss. © 2008 Elsevier Inc.
 
ISSN8756-3282
2012 Impact Factor: 3.823
2012 SCImago Journal Rankings: 1.315
 
DOIhttp://dx.doi.org/10.1016/j.bone.2008.04.018
 
ISI Accession Number IDWOS:000258953800021
Funding AgencyGrant Number
HKU Foundation Bone Health Fund CRCG
Osteoporosis Research Fund of The University of Hong Kong
NIH National Center for Complementary and Alternative MedicineR21 AT01957
Funding Information:

This study is supported by HKU Foundation Bone Health Fund CRCG grant and Osteoporosis Research Fund of The University of Hong Kong. We would like to thank NIH National Center for Complementary and Alternative Medicine grant (R21 AT01957) for the Support of F Kronenbergg, EJ Kennelly and B Jiang.

 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorChan, BY
 
dc.contributor.authorLau, KS
 
dc.contributor.authorJiang, B
 
dc.contributor.authorKennelly, EJ
 
dc.contributor.authorKronenberg, F
 
dc.contributor.authorKung, AWC
 
dc.date.accessioned2010-09-06T07:21:17Z
 
dc.date.available2010-09-06T07:21:17Z
 
dc.date.issued2008
 
dc.description.abstractAceaea racemosa (formerly Cimicifuga racemosa, black cohosh, AR) extracts have been widely used as an alternative to hormonal replacement therapy for menopausal symptoms. Recent evidences suggest AR extracts are also effective in protecting against postmenopausal bone loss. To determine whether AR has any direct anabolic effect on osteoblasts, we investigated the ethanolic extract of AR on bone nodule formation in mouse MC3T3-E1 preosteoblast cells. AR did not stimulate osteoblast proliferation. Rather, at high doses of 1000 ng/mL for 48 h, AR suppressed (7.2 ± 0.9% vs. control) osteoblast proliferation. At 500 ng/mL, a significant increase in bone nodule formation was seen with Von Kossa staining. Using quantitative PCR analysis, AR was shown to enhance the gene expression of runx2 and osteocalcin. Co-treatment with ICI 182,780, the selective estrogen receptor antagonist, abolished the stimulatory effect of AR on runx2 and osteocalcin gene induction, as well as on bone nodule formation in MC3T3-E1 cells. This is a first report of the direct effect of AR on enhancement of bone nodule formation in osteoblasts, and this action was mediated via an estrogen receptor-dependent mechanism. The results provide a scientific rationale at the molecular level for the claim that AR can offer effective prevention of postmenopausal bone loss. © 2008 Elsevier Inc.
 
dc.description.natureLink_to_subscribed_fulltext
 
dc.identifier.citationBone, 2008, v. 43 n. 3, p. 567-573 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.bone.2008.04.018
 
dc.identifier.citeulike2989744
 
dc.identifier.doihttp://dx.doi.org/10.1016/j.bone.2008.04.018
 
dc.identifier.epage573
 
dc.identifier.hkuros146151
 
dc.identifier.isiWOS:000258953800021
Funding AgencyGrant Number
HKU Foundation Bone Health Fund CRCG
Osteoporosis Research Fund of The University of Hong Kong
NIH National Center for Complementary and Alternative MedicineR21 AT01957
Funding Information:

This study is supported by HKU Foundation Bone Health Fund CRCG grant and Osteoporosis Research Fund of The University of Hong Kong. We would like to thank NIH National Center for Complementary and Alternative Medicine grant (R21 AT01957) for the Support of F Kronenbergg, EJ Kennelly and B Jiang.

 
dc.identifier.issn8756-3282
2012 Impact Factor: 3.823
2012 SCImago Journal Rankings: 1.315
 
dc.identifier.issue3
 
dc.identifier.openurl
 
dc.identifier.pmid18555764
 
dc.identifier.scopuseid_2-s2.0-49149112633
 
dc.identifier.spage567
 
dc.identifier.urihttp://hdl.handle.net/10722/76443
 
dc.identifier.volume43
 
dc.languageeng
 
dc.publisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/bone
 
dc.publisher.placeUnited States
 
dc.relation.ispartofBone
 
dc.relation.referencesReferences in Scopus
 
dc.rightsBone. Copyright © Elsevier Inc.
 
dc.subject.meshAnimals
 
dc.subject.meshBone and Bones - metabolism
 
dc.subject.meshCell Proliferation
 
dc.subject.meshCimicifuga - metabolism
 
dc.subject.meshCore Binding Factor Alpha 1 Subunit - biosynthesis
 
dc.subject.meshDose-Response Relationship, Drug
 
dc.subject.meshEstradiol - analogs & derivatives - pharmacology
 
dc.subject.meshEstrogen Antagonists - pharmacology
 
dc.subject.meshEthanol - pharmacology
 
dc.subject.meshMice
 
dc.subject.meshOsteoblasts - cytology - metabolism
 
dc.subject.meshOsteocalcin - biosynthesis
 
dc.subject.meshOsteogenesis - drug effects
 
dc.subject.meshPlant Extracts - pharmacology
 
dc.subject.meshReceptors, Androgen - metabolism
 
dc.titleEthanolic extract of Actaea racemosa (black cohosh) potentiates bone nodule formation in MC3T3-E1 preosteoblast cells
 
dc.typeArticle
 
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Author Affiliations
  1. Lehman College
  2. The University of Hong Kong
  3. Columbia University, College of Physicians and Surgeons