Article: Ethanolic extract of Actaea racemosa (black cohosh) potentiates bone nodule formation in MC3T3-E1 preosteoblast cells
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- Publisher Website: 10.1016/j.bone.2008.04.018
- Scopus: eid_2-s2.0-49149112633
- PMID: 18555764
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| Title | Ethanolic extract of Actaea racemosa (black cohosh) potentiates bone nodule formation in MC3T3-E1 preosteoblast cells | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| Authors | Chan, BY2 Lau, KS2 Jiang, B1 3 Kennelly, EJ1 Kronenberg, F3 Kung, AWC2 | ||||||||
| Issue Date | 2008 | ||||||||
| Publisher | Elsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/bone | ||||||||
| Citation | Bone, 2008, v. 43 n. 3, p. 567-573 [How to Cite?] DOI: http://dx.doi.org/10.1016/j.bone.2008.04.018 | ||||||||
| Abstract | Aceaea racemosa (formerly Cimicifuga racemosa, black cohosh, AR) extracts have been widely used as an alternative to hormonal replacement therapy for menopausal symptoms. Recent evidences suggest AR extracts are also effective in protecting against postmenopausal bone loss. To determine whether AR has any direct anabolic effect on osteoblasts, we investigated the ethanolic extract of AR on bone nodule formation in mouse MC3T3-E1 preosteoblast cells. AR did not stimulate osteoblast proliferation. Rather, at high doses of 1000 ng/mL for 48 h, AR suppressed (7.2 ± 0.9% vs. control) osteoblast proliferation. At 500 ng/mL, a significant increase in bone nodule formation was seen with Von Kossa staining. Using quantitative PCR analysis, AR was shown to enhance the gene expression of runx2 and osteocalcin. Co-treatment with ICI 182,780, the selective estrogen receptor antagonist, abolished the stimulatory effect of AR on runx2 and osteocalcin gene induction, as well as on bone nodule formation in MC3T3-E1 cells. This is a first report of the direct effect of AR on enhancement of bone nodule formation in osteoblasts, and this action was mediated via an estrogen receptor-dependent mechanism. The results provide a scientific rationale at the molecular level for the claim that AR can offer effective prevention of postmenopausal bone loss. © 2008 Elsevier Inc. | ||||||||
| ISSN | 8756-3282 2011 Impact Factor: 4.023 2011 SCImago Journal Rankings: 0.326 | ||||||||
| DOI | http://dx.doi.org/10.1016/j.bone.2008.04.018 | ||||||||
| ISI Accession Number ID | WOS:000258953800021
Funding Information: This study is supported by HKU Foundation Bone Health Fund CRCG grant and Osteoporosis Research Fund of The University of Hong Kong. We would like to thank NIH National Center for Complementary and Alternative Medicine grant (R21 AT01957) for the Support of F Kronenbergg, EJ Kennelly and B Jiang. | ||||||||
| References | References in Scopus |
| dc.contributor.author | Chan, BY | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| dc.contributor.author | Lau, KS | ||||||||
| dc.contributor.author | Jiang, B | ||||||||
| dc.contributor.author | Kennelly, EJ | ||||||||
| dc.contributor.author | Kronenberg, F | ||||||||
| dc.contributor.author | Kung, AWC | ||||||||
| dc.date.accessioned | 2010-09-06T07:21:17Z | ||||||||
| dc.date.available | 2010-09-06T07:21:17Z | ||||||||
| dc.date.issued | 2008 | ||||||||
| dc.description.abstract | Aceaea racemosa (formerly Cimicifuga racemosa, black cohosh, AR) extracts have been widely used as an alternative to hormonal replacement therapy for menopausal symptoms. Recent evidences suggest AR extracts are also effective in protecting against postmenopausal bone loss. To determine whether AR has any direct anabolic effect on osteoblasts, we investigated the ethanolic extract of AR on bone nodule formation in mouse MC3T3-E1 preosteoblast cells. AR did not stimulate osteoblast proliferation. Rather, at high doses of 1000 ng/mL for 48 h, AR suppressed (7.2 ± 0.9% vs. control) osteoblast proliferation. At 500 ng/mL, a significant increase in bone nodule formation was seen with Von Kossa staining. Using quantitative PCR analysis, AR was shown to enhance the gene expression of runx2 and osteocalcin. Co-treatment with ICI 182,780, the selective estrogen receptor antagonist, abolished the stimulatory effect of AR on runx2 and osteocalcin gene induction, as well as on bone nodule formation in MC3T3-E1 cells. This is a first report of the direct effect of AR on enhancement of bone nodule formation in osteoblasts, and this action was mediated via an estrogen receptor-dependent mechanism. The results provide a scientific rationale at the molecular level for the claim that AR can offer effective prevention of postmenopausal bone loss. © 2008 Elsevier Inc. | ||||||||
| dc.description.nature | Link_to_subscribed_fulltext | ||||||||
| dc.identifier.citation | Bone, 2008, v. 43 n. 3, p. 567-573 [How to Cite?] DOI: http://dx.doi.org/10.1016/j.bone.2008.04.018 | ||||||||
| dc.identifier.citeulike | 2989744 | ||||||||
| dc.identifier.doi | http://dx.doi.org/10.1016/j.bone.2008.04.018 | ||||||||
| dc.identifier.epage | 573 | ||||||||
| dc.identifier.hkuros | 146151 | ||||||||
| dc.identifier.isi | WOS:000258953800021
Funding Information: This study is supported by HKU Foundation Bone Health Fund CRCG grant and Osteoporosis Research Fund of The University of Hong Kong. We would like to thank NIH National Center for Complementary and Alternative Medicine grant (R21 AT01957) for the Support of F Kronenbergg, EJ Kennelly and B Jiang. | ||||||||
| dc.identifier.issn | 8756-3282 2011 Impact Factor: 4.023 2011 SCImago Journal Rankings: 0.326 | ||||||||
| dc.identifier.issue | 3 | ||||||||
| dc.identifier.openurl | | ||||||||
| dc.identifier.pmid | 18555764 | ||||||||
| dc.identifier.scopus | eid_2-s2.0-49149112633 | ||||||||
| dc.identifier.spage | 567 | ||||||||
| dc.identifier.uri | http://hdl.handle.net/10722/76443 | ||||||||
| dc.identifier.volume | 43 | ||||||||
| dc.language | eng | ||||||||
| dc.publisher | Elsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/bone | ||||||||
| dc.publisher.place | United States | ||||||||
| dc.relation.ispartof | Bone | ||||||||
| dc.relation.references | References in Scopus | ||||||||
| dc.rights | Bone. Copyright © Elsevier Inc. | ||||||||
| dc.subject.mesh | Animals | ||||||||
| dc.subject.mesh | Bone and Bones - metabolism | ||||||||
| dc.subject.mesh | Cell Proliferation | ||||||||
| dc.subject.mesh | Cimicifuga - metabolism | ||||||||
| dc.subject.mesh | Core Binding Factor Alpha 1 Subunit - biosynthesis | ||||||||
| dc.subject.mesh | Dose-Response Relationship, Drug | ||||||||
| dc.subject.mesh | Estradiol - analogs & derivatives - pharmacology | ||||||||
| dc.subject.mesh | Estrogen Antagonists - pharmacology | ||||||||
| dc.subject.mesh | Ethanol - pharmacology | ||||||||
| dc.subject.mesh | Mice | ||||||||
| dc.subject.mesh | Osteoblasts - cytology - metabolism | ||||||||
| dc.subject.mesh | Osteocalcin - biosynthesis | ||||||||
| dc.subject.mesh | Osteogenesis - drug effects | ||||||||
| dc.subject.mesh | Plant Extracts - pharmacology | ||||||||
| dc.subject.mesh | Receptors, Androgen - metabolism | ||||||||
| dc.title | Ethanolic extract of Actaea racemosa (black cohosh) potentiates bone nodule formation in MC3T3-E1 preosteoblast cells | ||||||||
| dc.type | Article |
Author Affiliations
- Lehman College
- The University of Hong Kong
- Columbia University, College of Physicians and Surgeons