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Article: Functional ion channels in mouse bone marrow mesenchymal stem cells

TitleFunctional ion channels in mouse bone marrow mesenchymal stem cells
Authors
Issue Date2007
PublisherAmerican Physiological Society. The Journal's web site is located at http://intl-ajpcell.physiology.org/
Citation
American Journal Of Physiology - Cell Physiology, 2007, v. 293 n. 5, p. C1561-C1567 How to Cite?
AbstractBone marrow mesenchymal stem cells (MSCs) are used as a cell source for cardiomyoplasty; however, the cellular electrophysiological properties are not fully understood. The present study was to investigate the functional ionic channels in undifferentiated mouse bone marrow MSCs using whole cell patch-voltage clamp technique, RT-PCR, and Western immunoblotting analysis. We found that three types of ionic currents were present in mouse MSCs, including a Ca2+-activated K+ current (IKCa), an inwardly rectifying K+ current (IKir), and a chloride current (ICl). IKir was inhibited by Ba2+, and I KCa was activated by the Ca2+ ionophore A-23187 and inhibited by the intermediate-conductance IKCa channel blocker clotrimazole. ICl was activated by hyposmotic (0.8 T) conditions and inhibited by the chloride channel blockers DIDS and NPPB. The corresponding ion channel genes and proteins, KCa3.1 for IKCa, Kir2.1 for I Kir, and Clcn3 for ICl, were confirmed by RT-PCR and Western immunoblotting analysis in mouse MSCs. These results demonstrate that three types of functional ion channel currents (i.e., IKir, I KCa, and ICl) are present in mouse bone marrow MSCs. Copyright © 2007 the American Physiological Society.
Persistent Identifierhttp://hdl.handle.net/10722/76415
ISSN
2015 Impact Factor: 3.395
2015 SCImago Journal Rankings: 1.893
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorTao, Ren_HK
dc.contributor.authorLau, CPen_HK
dc.contributor.authorTse, HFen_HK
dc.contributor.authorLi, GRen_HK
dc.date.accessioned2010-09-06T07:20:59Z-
dc.date.available2010-09-06T07:20:59Z-
dc.date.issued2007en_HK
dc.identifier.citationAmerican Journal Of Physiology - Cell Physiology, 2007, v. 293 n. 5, p. C1561-C1567en_HK
dc.identifier.issn0363-6143en_HK
dc.identifier.urihttp://hdl.handle.net/10722/76415-
dc.description.abstractBone marrow mesenchymal stem cells (MSCs) are used as a cell source for cardiomyoplasty; however, the cellular electrophysiological properties are not fully understood. The present study was to investigate the functional ionic channels in undifferentiated mouse bone marrow MSCs using whole cell patch-voltage clamp technique, RT-PCR, and Western immunoblotting analysis. We found that three types of ionic currents were present in mouse MSCs, including a Ca2+-activated K+ current (IKCa), an inwardly rectifying K+ current (IKir), and a chloride current (ICl). IKir was inhibited by Ba2+, and I KCa was activated by the Ca2+ ionophore A-23187 and inhibited by the intermediate-conductance IKCa channel blocker clotrimazole. ICl was activated by hyposmotic (0.8 T) conditions and inhibited by the chloride channel blockers DIDS and NPPB. The corresponding ion channel genes and proteins, KCa3.1 for IKCa, Kir2.1 for I Kir, and Clcn3 for ICl, were confirmed by RT-PCR and Western immunoblotting analysis in mouse MSCs. These results demonstrate that three types of functional ion channel currents (i.e., IKir, I KCa, and ICl) are present in mouse bone marrow MSCs. Copyright © 2007 the American Physiological Society.en_HK
dc.languageengen_HK
dc.publisherAmerican Physiological Society. The Journal's web site is located at http://intl-ajpcell.physiology.org/en_HK
dc.relation.ispartofAmerican Journal of Physiology - Cell Physiologyen_HK
dc.subject.mesh4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid - pharmacologyen_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshBarium Compounds - metabolismen_HK
dc.subject.meshBlotting, Westernen_HK
dc.subject.meshBone Marrow Cells - drug effects - metabolismen_HK
dc.subject.meshCalcimycin - pharmacologyen_HK
dc.subject.meshCell Sizeen_HK
dc.subject.meshCells, Cultureden_HK
dc.subject.meshChloride Channels - drug effects - genetics - metabolismen_HK
dc.subject.meshChlorides - metabolismen_HK
dc.subject.meshClotrimazole - pharmacologyen_HK
dc.subject.meshIntermediate-Conductance Calcium-Activated Potassium Channels - drug effects - genetics - metabolismen_HK
dc.subject.meshIonophores - pharmacologyen_HK
dc.subject.meshMembrane Potentialsen_HK
dc.subject.meshMesenchymal Stem Cells - drug effects - metabolismen_HK
dc.subject.meshMiceen_HK
dc.subject.meshMice, Inbred C57BLen_HK
dc.subject.meshNitrobenzoates - pharmacologyen_HK
dc.subject.meshPatch-Clamp Techniquesen_HK
dc.subject.meshPotassium - metabolismen_HK
dc.subject.meshPotassium Channel Blockers - pharmacologyen_HK
dc.subject.meshPotassium Channels, Inwardly Rectifying - drug effects - genetics - metabolismen_HK
dc.subject.meshRNA, Messenger - metabolismen_HK
dc.subject.meshReverse Transcriptase Polymerase Chain Reactionen_HK
dc.titleFunctional ion channels in mouse bone marrow mesenchymal stem cellsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0363-6143&volume=293&issue=5&spage=C1561&epage=7&date=2007&atitle=Functional+Ion+Channels+in+Mouse+Bone+Marrow+Mesenchymal+Stem+Cells.+en_HK
dc.identifier.emailTse, HF:hftse@hkucc.hku.hken_HK
dc.identifier.emailLi, GR:grli@hkucc.hku.hken_HK
dc.identifier.authorityTse, HF=rp00428en_HK
dc.identifier.authorityLi, GR=rp00476en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1152/ajpcell.00240.2007en_HK
dc.identifier.pmid17699636-
dc.identifier.scopuseid_2-s2.0-36049009956en_HK
dc.identifier.hkuros139379en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-36049009956&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume293en_HK
dc.identifier.issue5en_HK
dc.identifier.spageC1561en_HK
dc.identifier.epageC1567en_HK
dc.identifier.isiWOS:000250709500015-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridTao, R=7102857104en_HK
dc.identifier.scopusauthoridLau, CP=7401968501en_HK
dc.identifier.scopusauthoridTse, HF=7006070805en_HK
dc.identifier.scopusauthoridLi, GR=7408462932en_HK
dc.identifier.citeulike10063198-

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