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Article: Aberrant gene promoter methylation in acute promyelocytic leukaemia: Profile and prognostic significance

TitleAberrant gene promoter methylation in acute promyelocytic leukaemia: Profile and prognostic significance
Authors
Issue Date2003
PublisherBlackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/BJH
Citation
British Journal Of Haematology, 2003, v. 122 n. 4, p. 571-578 How to Cite?
AbstractAcute promyelocytic leukaemia (APL) has distinct clinicopathological and molecular features, However, the profile of aberrant gene promoter methylation is undefined. In this study, methylation-specific polymerase chain reaction (MSP) was used to define the methylation status of a panel of nine genes, comprising p 15, p 16, RARβ, oestrogen receptor (ER). E-cadherin (E-CAD), p73. caspase 8 (CASP8), VHL and MGMT, in 29 patients with APL. Aberrant methylation of p15, ER, RARβ, p16 and E-CAD occurred, respectively, in 23 (79%), 14 (48%), six (21%), six (21%) and two (7%) patients at diagnosis, but p73, VHL. CASP8 and MGMT were not methylated in any patients. There was methylation of one gene in 13 patients (45%), two genes in four patients (14%), three genes in six patients (21%) and four genes in three patients (10%). Concurrent methylation of two or more genes occurred in 13 patients (45%). No association was identified between gene methylation and presenting clinicopathological features. However, p15 methylation was significantly associated with an inferior disease-free survival (DFS, P = 0.008), and remained the only poor prognostic factor in multivariate analysis (P = 0.019). In APL, p15, p16, ER and RARβ were most frequently methylated. This profile is distinct from other types of myeloid leukaemias, p15 methylation has a poor prognostic impact on DFS.
Persistent Identifierhttp://hdl.handle.net/10722/76400
ISSN
2015 Impact Factor: 5.401
2015 SCImago Journal Rankings: 2.313
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorChim, CSen_HK
dc.contributor.authorWong, SYen_HK
dc.contributor.authorKwong, YLen_HK
dc.date.accessioned2010-09-06T07:20:49Z-
dc.date.available2010-09-06T07:20:49Z-
dc.date.issued2003en_HK
dc.identifier.citationBritish Journal Of Haematology, 2003, v. 122 n. 4, p. 571-578en_HK
dc.identifier.issn0007-1048en_HK
dc.identifier.urihttp://hdl.handle.net/10722/76400-
dc.description.abstractAcute promyelocytic leukaemia (APL) has distinct clinicopathological and molecular features, However, the profile of aberrant gene promoter methylation is undefined. In this study, methylation-specific polymerase chain reaction (MSP) was used to define the methylation status of a panel of nine genes, comprising p 15, p 16, RARβ, oestrogen receptor (ER). E-cadherin (E-CAD), p73. caspase 8 (CASP8), VHL and MGMT, in 29 patients with APL. Aberrant methylation of p15, ER, RARβ, p16 and E-CAD occurred, respectively, in 23 (79%), 14 (48%), six (21%), six (21%) and two (7%) patients at diagnosis, but p73, VHL. CASP8 and MGMT were not methylated in any patients. There was methylation of one gene in 13 patients (45%), two genes in four patients (14%), three genes in six patients (21%) and four genes in three patients (10%). Concurrent methylation of two or more genes occurred in 13 patients (45%). No association was identified between gene methylation and presenting clinicopathological features. However, p15 methylation was significantly associated with an inferior disease-free survival (DFS, P = 0.008), and remained the only poor prognostic factor in multivariate analysis (P = 0.019). In APL, p15, p16, ER and RARβ were most frequently methylated. This profile is distinct from other types of myeloid leukaemias, p15 methylation has a poor prognostic impact on DFS.en_HK
dc.languageengen_HK
dc.publisherBlackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/BJHen_HK
dc.relation.ispartofBritish Journal of Haematologyen_HK
dc.rightsBritish Journal of Haematology. Copyright © Blackwell Publishing Ltd.en_HK
dc.subject.meshAdolescenten_HK
dc.subject.meshAdulten_HK
dc.subject.meshAgeden_HK
dc.subject.meshBase Sequenceen_HK
dc.subject.meshDNA Methylationen_HK
dc.subject.meshDNA, Neoplasm - geneticsen_HK
dc.subject.meshFemaleen_HK
dc.subject.meshGenes, Tumor Suppressoren_HK
dc.subject.meshHumansen_HK
dc.subject.meshLeukemia, Promyelocytic, Acute - genetics - pathologyen_HK
dc.subject.meshMaleen_HK
dc.subject.meshMiddle Ageden_HK
dc.subject.meshMolecular Sequence Dataen_HK
dc.subject.meshNeoplasm Proteins - geneticsen_HK
dc.subject.meshNeoplasm, Residualen_HK
dc.subject.meshPolymerase Chain Reaction - methodsen_HK
dc.subject.meshPrognosisen_HK
dc.subject.meshPromoter Regions, Genetic - geneticsen_HK
dc.subject.meshSequence Alignmenten_HK
dc.subject.meshSurvival Analysisen_HK
dc.subject.meshTumor Markers, Biological - geneticsen_HK
dc.titleAberrant gene promoter methylation in acute promyelocytic leukaemia: Profile and prognostic significanceen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0007-1048&volume=122&issue=4&spage=571&epage=578&date=2003&atitle=Aberrant+gene+promoter+methylation+in+acute+promyelocytic+leukaemia:+profile+and+prognostic+significanceen_HK
dc.identifier.emailChim, CS:jcschim@hku.hken_HK
dc.identifier.emailKwong, YL:ylkwong@hku.hken_HK
dc.identifier.authorityChim, CS=rp00408en_HK
dc.identifier.authorityKwong, YL=rp00358en_HK
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1046/j.1365-2141.2003.04462.xen_HK
dc.identifier.pmid12899712-
dc.identifier.scopuseid_2-s2.0-0043163772en_HK
dc.identifier.hkuros88682en_HK
dc.identifier.hkuros87919-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0043163772&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume122en_HK
dc.identifier.issue4en_HK
dc.identifier.spage571en_HK
dc.identifier.epage578en_HK
dc.identifier.isiWOS:000184573300005-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridChim, CS=7004597253en_HK
dc.identifier.scopusauthoridWong, SY=7404589866en_HK
dc.identifier.scopusauthoridKwong, YL=7102818954en_HK

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