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Article: Characterization of expanded intermediate cell mass in zebrafish chordin morphant embryos

TitleCharacterization of expanded intermediate cell mass in zebrafish chordin morphant embryos
Authors
Issue Date2005
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/ydbio
Citation
Developmental Biology, 2005, v. 277 n. 1, p. 235-254 How to Cite?
AbstractWe investigated the mechanisms of intermediate cell mass (ICM) expansion in zebrafish chordin (Chd) morphant embryos and examined the role of BMPs in relation to this phenotype. At 24 h post-fertilization (hpf), the expanded ICM of embryos injected with chd morpholino (MO) (Chd MO embryos) contained a monotonous population of hematopoietic progenitors. In situ hybridization showed that hematopoietic transcription factors were ubiquitously expressed in the ICM whereas vascular gene expression was confined to the periphery. BMP4 (but not BMP2b or 7) and smad5 mRNA were ectopically expressed in the Chd MO ICM. At 48 hpf, monocytic cells were evident in both the ICM and circulation of Chd MO but not WT embryos. While injection of BMP4 MO had no effect on WT hematopoiesis, co-injecting BMP4 with chd MOs significantly reduced ICM expansion. Microarray studies revealed a number of genes that were differentially expressed in Chd MO and WT embryos and their roles in hematopoiesis has yet to be determined. In conclusion, the expanded ICM in Chd MO embryos represented an expansion of embryonic hematopoiesis that was skewed towards a monocytic lineage. BMP4, but not BMP2b or 7, was involved in this process. The results provide ground for further research into the mechanisms of embryonic hematopoietic cell expansion. © 2004 Elsevier Inc. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/76317
ISSN
2015 Impact Factor: 3.155
2015 SCImago Journal Rankings: 2.554
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLeung, AYHen_HK
dc.contributor.authorMendenhall, EMen_HK
dc.contributor.authorKwan, TTFen_HK
dc.contributor.authorLiang, Ren_HK
dc.contributor.authorEckfeldt, Cen_HK
dc.contributor.authorChen, Een_HK
dc.contributor.authorHammerschmidt, Men_HK
dc.contributor.authorGrindley, Sen_HK
dc.contributor.authorEkker, SCen_HK
dc.contributor.authorVerfaillie, CMen_HK
dc.date.accessioned2010-09-06T07:19:57Z-
dc.date.available2010-09-06T07:19:57Z-
dc.date.issued2005en_HK
dc.identifier.citationDevelopmental Biology, 2005, v. 277 n. 1, p. 235-254en_HK
dc.identifier.issn0012-1606en_HK
dc.identifier.urihttp://hdl.handle.net/10722/76317-
dc.description.abstractWe investigated the mechanisms of intermediate cell mass (ICM) expansion in zebrafish chordin (Chd) morphant embryos and examined the role of BMPs in relation to this phenotype. At 24 h post-fertilization (hpf), the expanded ICM of embryos injected with chd morpholino (MO) (Chd MO embryos) contained a monotonous population of hematopoietic progenitors. In situ hybridization showed that hematopoietic transcription factors were ubiquitously expressed in the ICM whereas vascular gene expression was confined to the periphery. BMP4 (but not BMP2b or 7) and smad5 mRNA were ectopically expressed in the Chd MO ICM. At 48 hpf, monocytic cells were evident in both the ICM and circulation of Chd MO but not WT embryos. While injection of BMP4 MO had no effect on WT hematopoiesis, co-injecting BMP4 with chd MOs significantly reduced ICM expansion. Microarray studies revealed a number of genes that were differentially expressed in Chd MO and WT embryos and their roles in hematopoiesis has yet to be determined. In conclusion, the expanded ICM in Chd MO embryos represented an expansion of embryonic hematopoiesis that was skewed towards a monocytic lineage. BMP4, but not BMP2b or 7, was involved in this process. The results provide ground for further research into the mechanisms of embryonic hematopoietic cell expansion. © 2004 Elsevier Inc. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/ydbioen_HK
dc.relation.ispartofDevelopmental Biologyen_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshBone Morphogenetic Protein 4en_HK
dc.subject.meshBone Morphogenetic Proteins - physiologyen_HK
dc.subject.meshCell Divisionen_HK
dc.subject.meshDose-Response Relationship, Drugen_HK
dc.subject.meshGene Expression Profilingen_HK
dc.subject.meshGlycoproteins - physiologyen_HK
dc.subject.meshHematopoiesisen_HK
dc.subject.meshIntercellular Signaling Peptides and Proteins - physiologyen_HK
dc.subject.meshOligonucleotide Array Sequence Analysisen_HK
dc.subject.meshSignal Transductionen_HK
dc.subject.meshZebrafish - embryologyen_HK
dc.subject.meshZebrafish Proteinsen_HK
dc.titleCharacterization of expanded intermediate cell mass in zebrafish chordin morphant embryosen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0012-1606&volume=277&issue=1&spage=235&epage=254&date=2005&atitle=Characterization+of+expanded+intermediate+cell+mass+in+zebrafish+chordin+morphant+embryosen_HK
dc.identifier.emailLeung, AYH:ayhleung@hku.hken_HK
dc.identifier.emailLiang, R:rliang@hku.hken_HK
dc.identifier.authorityLeung, AYH=rp00265en_HK
dc.identifier.authorityLiang, R=rp00345en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.ydbio.2004.09.032en_HK
dc.identifier.pmid15572152-
dc.identifier.scopuseid_2-s2.0-9644294510en_HK
dc.identifier.hkuros99039en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-9644294510&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume277en_HK
dc.identifier.issue1en_HK
dc.identifier.spage235en_HK
dc.identifier.epage254en_HK
dc.identifier.isiWOS:000225741200018-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridLeung, AYH=7403012668en_HK
dc.identifier.scopusauthoridMendenhall, EM=9741377300en_HK
dc.identifier.scopusauthoridKwan, TTF=8776810800en_HK
dc.identifier.scopusauthoridLiang, R=26643224900en_HK
dc.identifier.scopusauthoridEckfeldt, C=6507708270en_HK
dc.identifier.scopusauthoridChen, E=7402316227en_HK
dc.identifier.scopusauthoridHammerschmidt, M=7006962776en_HK
dc.identifier.scopusauthoridGrindley, S=8776811300en_HK
dc.identifier.scopusauthoridEkker, SC=7003541150en_HK
dc.identifier.scopusauthoridVerfaillie, CM=7004524257en_HK
dc.identifier.citeulike11790760-

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