Article: 12-Lipoxygenase inhibition induced apoptosis in human gastric cancer cells

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Title12-Lipoxygenase inhibition induced apoptosis in human gastric cancer cells
AuthorsWong, BCY1
Wang, WP1
Cho, CH1
Fan, XM1
Lin, MCM1
Kung, HF1
Lam, SK1
Issue Date2001
PublisherOxford University Press. The Journal's web site is located at http://carcin.oxfordjournals.org/
CitationCarcinogenesis, 2001, v. 22 n. 9, p. 1349-1354 [How to Cite?]
AbstractArachidonic acid release from membrane phospholipids is essential for tumour cell proliferation. Lipoxygenases constitute a pathway for arachidonate metabolism. The present study investigated the expression of 12-lipoxygenase and its effect on cell proliferation as well as survival in two human gastric cancer cell lines (AGS and MKN-28). RT-PCR and western blots, respectively, showed 12-LOX mRNA and protein expression in both AGS and MKN-28 cell lines. Treatment with a 12-LOX inhibitor, baicalein, significantly inhibited cancer cell proliferation, but a metabolite of 12-LOX activity, 12 hydroxyeicosatetraenoic acid (12-HETE) reversed baicalein-induced growth inhibition. Furthermore, the blockade of the 12-LOX pathway through a 12-LOX inhibitor and antisense induced apoptosis of gastric cancer cell lines. The biochemical characteristics of apoptosis were p53-independent combined with a decrease in bcl-2 expression. Caspase-7 was proteolytically activated and responsible for the apoptosis execution.
ISSN0143-3334
2011 Impact Factor: 5.702
2011 SCImago Journal Rankings: 0.692
ISI Accession Number IDWOS:000171021100003
ReferencesReferences in Scopus
DC Field
Value
dc.contributor.authorWong, BCY
dc.contributor.authorWang, WP
dc.contributor.authorCho, CH
dc.contributor.authorFan, XM
dc.contributor.authorLin, MCM
dc.contributor.authorKung, HF
dc.contributor.authorLam, SK
dc.date.accessioned2010-09-06T07:19:36Z
dc.date.available2010-09-06T07:19:36Z
dc.date.issued2001
dc.description.abstractArachidonic acid release from membrane phospholipids is essential for tumour cell proliferation. Lipoxygenases constitute a pathway for arachidonate metabolism. The present study investigated the expression of 12-lipoxygenase and its effect on cell proliferation as well as survival in two human gastric cancer cell lines (AGS and MKN-28). RT-PCR and western blots, respectively, showed 12-LOX mRNA and protein expression in both AGS and MKN-28 cell lines. Treatment with a 12-LOX inhibitor, baicalein, significantly inhibited cancer cell proliferation, but a metabolite of 12-LOX activity, 12 hydroxyeicosatetraenoic acid (12-HETE) reversed baicalein-induced growth inhibition. Furthermore, the blockade of the 12-LOX pathway through a 12-LOX inhibitor and antisense induced apoptosis of gastric cancer cell lines. The biochemical characteristics of apoptosis were p53-independent combined with a decrease in bcl-2 expression. Caspase-7 was proteolytically activated and responsible for the apoptosis execution.
dc.description.natureLink_to_subscribed_fulltext
dc.identifier.citationCarcinogenesis, 2001, v. 22 n. 9, p. 1349-1354 [How to Cite?]
dc.identifier.epage1354
dc.identifier.hkuros68763
dc.identifier.isiWOS:000171021100003
dc.identifier.issn0143-3334
2011 Impact Factor: 5.702
2011 SCImago Journal Rankings: 0.692
dc.identifier.issue9
dc.identifier.openurl
dc.identifier.pmid11532854
dc.identifier.scopuseid_2-s2.0-0034811654
dc.identifier.spage1349
dc.identifier.urihttp://hdl.handle.net/10722/76285
dc.identifier.volume22
dc.languageeng
dc.publisherOxford University Press. The Journal's web site is located at http://carcin.oxfordjournals.org/
dc.publisher.placeUnited Kingdom
dc.relation.ispartofCarcinogenesis
dc.relation.referencesReferences in Scopus
dc.rightsCarcinogenesis. Copyright © Oxford University Press.
dc.subject.mesh12-Hydroxy-5,8,10,14-eicosatetraenoic Acid - pharmacology
dc.subject.meshApoptosis - drug effects - physiology
dc.subject.meshArachidonate 12-Lipoxygenase - biosynthesis - genetics
dc.subject.meshBlood Platelets - enzymology
dc.subject.meshBlotting, Western
dc.subject.meshCaspase 3
dc.subject.meshCaspase 7
dc.subject.meshCaspases - metabolism
dc.subject.meshCell Division - drug effects - physiology
dc.subject.meshDrug Interactions
dc.subject.meshFlavanones
dc.subject.meshFlavonoids - pharmacology
dc.subject.meshGene Expression Regulation, Neoplastic - drug effects
dc.subject.meshGrowth Inhibitors - pharmacology
dc.subject.meshHumans
dc.subject.meshLipoxygenase Inhibitors - pharmacology
dc.subject.meshRNA, Messenger - biosynthesis - genetics
dc.subject.meshStomach Neoplasms - enzymology - pathology
dc.subject.meshTumor Cells, Cultured
dc.title12-Lipoxygenase inhibition induced apoptosis in human gastric cancer cells
dc.typeArticle
Author Affiliations
  1. The University of Hong Kong