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Article: 12-Lipoxygenase inhibition induced apoptosis in human gastric cancer cells

Title12-Lipoxygenase inhibition induced apoptosis in human gastric cancer cells
Authors
Issue Date2001
PublisherOxford University Press. The Journal's web site is located at http://carcin.oxfordjournals.org/
Citation
Carcinogenesis, 2001, v. 22 n. 9, p. 1349-1354 How to Cite?
AbstractArachidonic acid release from membrane phospholipids is essential for tumour cell proliferation. Lipoxygenases constitute a pathway for arachidonate metabolism. The present study investigated the expression of 12-lipoxygenase and its effect on cell proliferation as well as survival in two human gastric cancer cell lines (AGS and MKN-28). RT-PCR and western blots, respectively, showed 12-LOX mRNA and protein expression in both AGS and MKN-28 cell lines. Treatment with a 12-LOX inhibitor, baicalein, significantly inhibited cancer cell proliferation, but a metabolite of 12-LOX activity, 12 hydroxyeicosatetraenoic acid (12-HETE) reversed baicalein-induced growth inhibition. Furthermore, the blockade of the 12-LOX pathway through a 12-LOX inhibitor and antisense induced apoptosis of gastric cancer cell lines. The biochemical characteristics of apoptosis were p53-independent combined with a decrease in bcl-2 expression. Caspase-7 was proteolytically activated and responsible for the apoptosis execution.
Persistent Identifierhttp://hdl.handle.net/10722/76285
ISSN
2014 Impact Factor: 5.334
2014 SCImago Journal Rankings: 2.152
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorWong, BCYen_HK
dc.contributor.authorWang, WPen_HK
dc.contributor.authorCho, CHen_HK
dc.contributor.authorFan, XMen_HK
dc.contributor.authorLin, MCMen_HK
dc.contributor.authorKung, HFen_HK
dc.contributor.authorLam, SKen_HK
dc.date.accessioned2010-09-06T07:19:36Z-
dc.date.available2010-09-06T07:19:36Z-
dc.date.issued2001en_HK
dc.identifier.citationCarcinogenesis, 2001, v. 22 n. 9, p. 1349-1354en_HK
dc.identifier.issn0143-3334en_HK
dc.identifier.urihttp://hdl.handle.net/10722/76285-
dc.description.abstractArachidonic acid release from membrane phospholipids is essential for tumour cell proliferation. Lipoxygenases constitute a pathway for arachidonate metabolism. The present study investigated the expression of 12-lipoxygenase and its effect on cell proliferation as well as survival in two human gastric cancer cell lines (AGS and MKN-28). RT-PCR and western blots, respectively, showed 12-LOX mRNA and protein expression in both AGS and MKN-28 cell lines. Treatment with a 12-LOX inhibitor, baicalein, significantly inhibited cancer cell proliferation, but a metabolite of 12-LOX activity, 12 hydroxyeicosatetraenoic acid (12-HETE) reversed baicalein-induced growth inhibition. Furthermore, the blockade of the 12-LOX pathway through a 12-LOX inhibitor and antisense induced apoptosis of gastric cancer cell lines. The biochemical characteristics of apoptosis were p53-independent combined with a decrease in bcl-2 expression. Caspase-7 was proteolytically activated and responsible for the apoptosis execution.en_HK
dc.languageengen_HK
dc.publisherOxford University Press. The Journal's web site is located at http://carcin.oxfordjournals.org/en_HK
dc.relation.ispartofCarcinogenesisen_HK
dc.rightsCarcinogenesis. Copyright © Oxford University Press.en_HK
dc.subject.mesh12-Hydroxy-5,8,10,14-eicosatetraenoic Acid - pharmacologyen_HK
dc.subject.meshApoptosis - drug effects - physiologyen_HK
dc.subject.meshArachidonate 12-Lipoxygenase - biosynthesis - geneticsen_HK
dc.subject.meshBlood Platelets - enzymologyen_HK
dc.subject.meshBlotting, Westernen_HK
dc.subject.meshCaspase 3en_HK
dc.subject.meshCaspase 7en_HK
dc.subject.meshCaspases - metabolismen_HK
dc.subject.meshCell Division - drug effects - physiologyen_HK
dc.subject.meshDrug Interactionsen_HK
dc.subject.meshFlavanonesen_HK
dc.subject.meshFlavonoids - pharmacologyen_HK
dc.subject.meshGene Expression Regulation, Neoplastic - drug effectsen_HK
dc.subject.meshGrowth Inhibitors - pharmacologyen_HK
dc.subject.meshHumansen_HK
dc.subject.meshLipoxygenase Inhibitors - pharmacologyen_HK
dc.subject.meshRNA, Messenger - biosynthesis - geneticsen_HK
dc.subject.meshStomach Neoplasms - enzymology - pathologyen_HK
dc.subject.meshTumor Cells, Cultureden_HK
dc.title12-Lipoxygenase inhibition induced apoptosis in human gastric cancer cellsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0143-3334&volume=22&issue=9&spage=1349&epage=54&date=2001&atitle=12-Lipoxygenase+inhibition+induced+apoptosis+in+human+gastric+cancer+cellsen_HK
dc.identifier.emailWong, BCY:bcywong@hku.hken_HK
dc.identifier.emailLin, MCM:mcllin@hkucc.hku.hken_HK
dc.identifier.authorityWong, BCY=rp00429en_HK
dc.identifier.authorityLin, MCM=rp00746en_HK
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1093/carcin/22.9.1349-
dc.identifier.pmid11532854-
dc.identifier.scopuseid_2-s2.0-0034811654en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0034811654&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume22en_HK
dc.identifier.issue9en_HK
dc.identifier.spage1349en_HK
dc.identifier.epage1354en_HK
dc.identifier.isiWOS:000171021100003-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridWong, BCY=7402023340en_HK
dc.identifier.scopusauthoridWang, WP=7501765704en_HK
dc.identifier.scopusauthoridCho, CH=14067000400en_HK
dc.identifier.scopusauthoridFan, XM=35187111100en_HK
dc.identifier.scopusauthoridLin, MCM=7404816359en_HK
dc.identifier.scopusauthoridKung, HF=7402514190en_HK
dc.identifier.scopusauthoridLam, SK=7402279473en_HK

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