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Article: 12-Lipoxygenase inhibition induced apoptosis in human gastric cancer cells
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Title12-Lipoxygenase inhibition induced apoptosis in human gastric cancer cells
 
AuthorsWong, BCY1
Wang, WP1
Cho, CH1
Fan, XM1
Lin, MCM1
Kung, HF1
Lam, SK1
 
Issue Date2001
 
PublisherOxford University Press. The Journal's web site is located at http://carcin.oxfordjournals.org/
 
CitationCarcinogenesis, 2001, v. 22 n. 9, p. 1349-1354 [How to Cite?]
DOI: http://dx.doi.org/10.1093/carcin/22.9.1349
 
AbstractArachidonic acid release from membrane phospholipids is essential for tumour cell proliferation. Lipoxygenases constitute a pathway for arachidonate metabolism. The present study investigated the expression of 12-lipoxygenase and its effect on cell proliferation as well as survival in two human gastric cancer cell lines (AGS and MKN-28). RT-PCR and western blots, respectively, showed 12-LOX mRNA and protein expression in both AGS and MKN-28 cell lines. Treatment with a 12-LOX inhibitor, baicalein, significantly inhibited cancer cell proliferation, but a metabolite of 12-LOX activity, 12 hydroxyeicosatetraenoic acid (12-HETE) reversed baicalein-induced growth inhibition. Furthermore, the blockade of the 12-LOX pathway through a 12-LOX inhibitor and antisense induced apoptosis of gastric cancer cell lines. The biochemical characteristics of apoptosis were p53-independent combined with a decrease in bcl-2 expression. Caspase-7 was proteolytically activated and responsible for the apoptosis execution.
 
ISSN0143-3334
2013 Impact Factor: 5.266
 
DOIhttp://dx.doi.org/10.1093/carcin/22.9.1349
 
ISI Accession Number IDWOS:000171021100003
 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorWong, BCY
 
dc.contributor.authorWang, WP
 
dc.contributor.authorCho, CH
 
dc.contributor.authorFan, XM
 
dc.contributor.authorLin, MCM
 
dc.contributor.authorKung, HF
 
dc.contributor.authorLam, SK
 
dc.date.accessioned2010-09-06T07:19:36Z
 
dc.date.available2010-09-06T07:19:36Z
 
dc.date.issued2001
 
dc.description.abstractArachidonic acid release from membrane phospholipids is essential for tumour cell proliferation. Lipoxygenases constitute a pathway for arachidonate metabolism. The present study investigated the expression of 12-lipoxygenase and its effect on cell proliferation as well as survival in two human gastric cancer cell lines (AGS and MKN-28). RT-PCR and western blots, respectively, showed 12-LOX mRNA and protein expression in both AGS and MKN-28 cell lines. Treatment with a 12-LOX inhibitor, baicalein, significantly inhibited cancer cell proliferation, but a metabolite of 12-LOX activity, 12 hydroxyeicosatetraenoic acid (12-HETE) reversed baicalein-induced growth inhibition. Furthermore, the blockade of the 12-LOX pathway through a 12-LOX inhibitor and antisense induced apoptosis of gastric cancer cell lines. The biochemical characteristics of apoptosis were p53-independent combined with a decrease in bcl-2 expression. Caspase-7 was proteolytically activated and responsible for the apoptosis execution.
 
dc.description.natureLink_to_OA_fulltext
 
dc.identifier.citationCarcinogenesis, 2001, v. 22 n. 9, p. 1349-1354 [How to Cite?]
DOI: http://dx.doi.org/10.1093/carcin/22.9.1349
 
dc.identifier.doihttp://dx.doi.org/10.1093/carcin/22.9.1349
 
dc.identifier.epage1354
 
dc.identifier.isiWOS:000171021100003
 
dc.identifier.issn0143-3334
2013 Impact Factor: 5.266
 
dc.identifier.issue9
 
dc.identifier.openurl
 
dc.identifier.pmid11532854
 
dc.identifier.scopuseid_2-s2.0-0034811654
 
dc.identifier.spage1349
 
dc.identifier.urihttp://hdl.handle.net/10722/76285
 
dc.identifier.volume22
 
dc.languageeng
 
dc.publisherOxford University Press. The Journal's web site is located at http://carcin.oxfordjournals.org/
 
dc.publisher.placeUnited Kingdom
 
dc.relation.ispartofCarcinogenesis
 
dc.relation.referencesReferences in Scopus
 
dc.rightsCarcinogenesis. Copyright © Oxford University Press.
 
dc.subject.mesh12-Hydroxy-5,8,10,14-eicosatetraenoic Acid - pharmacology
 
dc.subject.meshApoptosis - drug effects - physiology
 
dc.subject.meshArachidonate 12-Lipoxygenase - biosynthesis - genetics
 
dc.subject.meshBlood Platelets - enzymology
 
dc.subject.meshBlotting, Western
 
dc.subject.meshCaspase 3
 
dc.subject.meshCaspase 7
 
dc.subject.meshCaspases - metabolism
 
dc.subject.meshCell Division - drug effects - physiology
 
dc.subject.meshDrug Interactions
 
dc.subject.meshFlavanones
 
dc.subject.meshFlavonoids - pharmacology
 
dc.subject.meshGene Expression Regulation, Neoplastic - drug effects
 
dc.subject.meshGrowth Inhibitors - pharmacology
 
dc.subject.meshHumans
 
dc.subject.meshLipoxygenase Inhibitors - pharmacology
 
dc.subject.meshRNA, Messenger - biosynthesis - genetics
 
dc.subject.meshStomach Neoplasms - enzymology - pathology
 
dc.subject.meshTumor Cells, Cultured
 
dc.title12-Lipoxygenase inhibition induced apoptosis in human gastric cancer cells
 
dc.typeArticle
 
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Author Affiliations
  1. The University of Hong Kong