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Article: Effect of dexamethasone on cultured human tenocytes and its reversibility by platelet-derived growth factor

TitleEffect of dexamethasone on cultured human tenocytes and its reversibility by platelet-derived growth factor
Authors
Issue Date2003
PublisherJournal of Bone and Joint Surgery. The Journal's web site is located at http://www.jbjs.org
Citation
Journal Of Bone And Joint Surgery - Series A, 2003, v. 85 n. 10, p. 1914-1920 How to Cite?
AbstractBackground: Many cases of tendon rupture after glucocorticoid injections have been reported in the literature. Despite previous studies on the histological and biomechanical changes in tendons after glucocorticoid injections, the role of glucocorticoid in causing tendon rupture still remains controversial. The objective of this study was to determine whether glucocorticoid has deleterious effects on the cellular metabolism and collagen production of cultured human tenocytes and the reversibility of these effects by platelet-derived growth factor-BB (PDGFBB). Methods: Primary cultures of human tenocytes obtained from explants of healthy patellar tendon, harvested during anterior cruciate ligament reconstructions, were performed. The effects on cell viability, cell proliferation, and induction of apoptosis were measured by [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay, 5-bromo-deoxyuridine incorporation, and DNA fragmentation assay, respectively. The effect on collagen synthesis was measured by 3H-proline incorporation assay. Results: The number of viable cells was decreased, in a dose-dependent manner, by the administration of 10 -9 to 10-4-M dexamethasone. This dose range also suppressed cell proliferation. No apoptotic effect was detected. Treatment with 10-6-M dexamethasone significantly reduced the amount of collagen synthesis. Co-incubation with 10 ng/mL of PDGFBB significantly reversed the effects caused by 10-6-M dexamethasone. Conclusions: Dexamethasone significantly decreased cell viability, suppressed cell proliferation, and reduced collagen synthesis in cultured human tenocytes. The effects were reversed by the simultaneous administration of PDGFBB. Clinical Relevance: The current study demonstrates that glucocorticoids adversely affect human tenocytes in cell culture. As the effects are reversible with simultaneous administration of PDGFBB, the growth factor may be useful clinically as a protective agent for patients receiving local glucocorticoid injections.
Persistent Identifierhttp://hdl.handle.net/10722/76086
ISSN
2015 Impact Factor: 5.163
2015 SCImago Journal Rankings: 2.938
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorWong, MWNen_HK
dc.contributor.authorTang, YYNen_HK
dc.contributor.authorLee, SKMen_HK
dc.contributor.authorFu, BSCen_HK
dc.contributor.authorChan, BPen_HK
dc.contributor.authorChan, CKMen_HK
dc.date.accessioned2010-09-06T07:17:30Z-
dc.date.available2010-09-06T07:17:30Z-
dc.date.issued2003en_HK
dc.identifier.citationJournal Of Bone And Joint Surgery - Series A, 2003, v. 85 n. 10, p. 1914-1920en_HK
dc.identifier.issn0021-9355en_HK
dc.identifier.urihttp://hdl.handle.net/10722/76086-
dc.description.abstractBackground: Many cases of tendon rupture after glucocorticoid injections have been reported in the literature. Despite previous studies on the histological and biomechanical changes in tendons after glucocorticoid injections, the role of glucocorticoid in causing tendon rupture still remains controversial. The objective of this study was to determine whether glucocorticoid has deleterious effects on the cellular metabolism and collagen production of cultured human tenocytes and the reversibility of these effects by platelet-derived growth factor-BB (PDGFBB). Methods: Primary cultures of human tenocytes obtained from explants of healthy patellar tendon, harvested during anterior cruciate ligament reconstructions, were performed. The effects on cell viability, cell proliferation, and induction of apoptosis were measured by [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay, 5-bromo-deoxyuridine incorporation, and DNA fragmentation assay, respectively. The effect on collagen synthesis was measured by 3H-proline incorporation assay. Results: The number of viable cells was decreased, in a dose-dependent manner, by the administration of 10 -9 to 10-4-M dexamethasone. This dose range also suppressed cell proliferation. No apoptotic effect was detected. Treatment with 10-6-M dexamethasone significantly reduced the amount of collagen synthesis. Co-incubation with 10 ng/mL of PDGFBB significantly reversed the effects caused by 10-6-M dexamethasone. Conclusions: Dexamethasone significantly decreased cell viability, suppressed cell proliferation, and reduced collagen synthesis in cultured human tenocytes. The effects were reversed by the simultaneous administration of PDGFBB. Clinical Relevance: The current study demonstrates that glucocorticoids adversely affect human tenocytes in cell culture. As the effects are reversible with simultaneous administration of PDGFBB, the growth factor may be useful clinically as a protective agent for patients receiving local glucocorticoid injections.en_HK
dc.languageengen_HK
dc.publisherJournal of Bone and Joint Surgery. The Journal's web site is located at http://www.jbjs.orgen_HK
dc.relation.ispartofJournal of Bone and Joint Surgery - Series Aen_HK
dc.subject.meshAnti-Inflammatory Agents - pharmacologyen_HK
dc.subject.meshAnticoagulants - pharmacologyen_HK
dc.subject.meshApoptosis - drug effectsen_HK
dc.subject.meshCell Division - drug effectsen_HK
dc.subject.meshCell Survival - drug effectsen_HK
dc.subject.meshCells, Cultureden_HK
dc.subject.meshCollagen - biosynthesis - drug effectsen_HK
dc.subject.meshDexamethasone - pharmacologyen_HK
dc.subject.meshDose-Response Relationship, Drugen_HK
dc.subject.meshHumansen_HK
dc.subject.meshPlatelet-Derived Growth Factor - pharmacologyen_HK
dc.subject.meshTendons - cytology - drug effects - metabolismen_HK
dc.subject.meshTime Factorsen_HK
dc.titleEffect of dexamethasone on cultured human tenocytes and its reversibility by platelet-derived growth factoren_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0021-9355&volume=85-A&issue=10&spage=1914&epage=1920.&date=2003&atitle=Effect+of+dexamethasone+on+cultured+human+tenocytes+and+its+reversibility+by+platelet-derived+growth+factoren_HK
dc.identifier.emailChan, BP:bpchan@hkucc.hku.hken_HK
dc.identifier.authorityChan, BP=rp00087en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.pmid14563798-
dc.identifier.scopuseid_2-s2.0-0141889331en_HK
dc.identifier.hkuros89218en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0141889331&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume85en_HK
dc.identifier.issue10en_HK
dc.identifier.spage1914en_HK
dc.identifier.epage1920en_HK
dc.identifier.isiWOS:000185972500008-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridWong, MWN=7403908619en_HK
dc.identifier.scopusauthoridTang, YYN=9942880700en_HK
dc.identifier.scopusauthoridLee, SKM=7601419361en_HK
dc.identifier.scopusauthoridFu, BSC=7402732268en_HK
dc.identifier.scopusauthoridChan, BP=7201530390en_HK
dc.identifier.scopusauthoridChan, CKM=7404813824en_HK

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