File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Morphology and adhesion of mesenchymal stem cells on PLLA, apatite and apatite/collagen surfaces

TitleMorphology and adhesion of mesenchymal stem cells on PLLA, apatite and apatite/collagen surfaces
Authors
Issue Date2008
PublisherSpringer New York LLC. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0957-4530
Citation
Journal Of Materials Science: Materials In Medicine, 2008, v. 19 n. 7, p. 2563-2567 How to Cite?
AbstractBiomimetic apatite/collagen composite coating, previously reported particularly with regard to its fabrication, characterization and interaction with osteoblast-like cells, has been investigated in this study to understand the response of human mesenchymal stem cells (hMSC) to such surface. PLLA films and PLLA films with apatite coating were compared with PLLA films with apatite/collagen composite coating. The hMSC morphology in response to such conditions was first observed using fluorescence microscopy. To further understand such cell-material interactions at a molecular level, integrin expression, actin assembly and vinculin-positive focal adhesion plaques were examined. Our results demonstrated that spreading of stem cells on the apatite/collagen composite surface was determined best among the three types of surfaces, followed by the apatite surface and then the PLLA control. Integrin expression on the apatite/collagen surface was higher than those on the apatite surface and PLLA surface. Immunostaining for vinculin and actin suggested that the composite coating on PLLA enhanced the formation of focal adhesion. © Springer Science+Business Media, LLC 2007.
Persistent Identifierhttp://hdl.handle.net/10722/75852
ISSN
2015 Impact Factor: 2.272
2013 SCImago Journal Rankings: 0.897
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorChen, Yen_HK
dc.contributor.authorCho, MRen_HK
dc.contributor.authorMak, AFTen_HK
dc.contributor.authorLi, JSen_HK
dc.contributor.authorWang, Men_HK
dc.contributor.authorSun, Sen_HK
dc.date.accessioned2010-09-06T07:15:12Z-
dc.date.available2010-09-06T07:15:12Z-
dc.date.issued2008en_HK
dc.identifier.citationJournal Of Materials Science: Materials In Medicine, 2008, v. 19 n. 7, p. 2563-2567en_HK
dc.identifier.issn0957-4530en_HK
dc.identifier.urihttp://hdl.handle.net/10722/75852-
dc.description.abstractBiomimetic apatite/collagen composite coating, previously reported particularly with regard to its fabrication, characterization and interaction with osteoblast-like cells, has been investigated in this study to understand the response of human mesenchymal stem cells (hMSC) to such surface. PLLA films and PLLA films with apatite coating were compared with PLLA films with apatite/collagen composite coating. The hMSC morphology in response to such conditions was first observed using fluorescence microscopy. To further understand such cell-material interactions at a molecular level, integrin expression, actin assembly and vinculin-positive focal adhesion plaques were examined. Our results demonstrated that spreading of stem cells on the apatite/collagen composite surface was determined best among the three types of surfaces, followed by the apatite surface and then the PLLA control. Integrin expression on the apatite/collagen surface was higher than those on the apatite surface and PLLA surface. Immunostaining for vinculin and actin suggested that the composite coating on PLLA enhanced the formation of focal adhesion. © Springer Science+Business Media, LLC 2007.en_HK
dc.languageengen_HK
dc.publisherSpringer New York LLC. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0957-4530en_HK
dc.relation.ispartofJournal of Materials Science: Materials in Medicineen_HK
dc.titleMorphology and adhesion of mesenchymal stem cells on PLLA, apatite and apatite/collagen surfacesen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0957-4530&volume=19&spage=2563&epage=2567&date=2008&atitle=Morphology+and+adhesion+of+mesenchymal+stem+cells+on+PLLA,+apatite+and+apatite/collagen+surfacesen_HK
dc.identifier.emailWang, M:memwang@hku.hken_HK
dc.identifier.authorityWang, M=rp00185en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1007/s10856-007-3195-2en_HK
dc.identifier.scopuseid_2-s2.0-73149103724en_HK
dc.identifier.hkuros148140en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-73149103724&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume19en_HK
dc.identifier.issue7en_HK
dc.identifier.spage2563en_HK
dc.identifier.epage2567en_HK
dc.identifier.isiWOS:000255879100006-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridChen, Y=8643007600en_HK
dc.identifier.scopusauthoridCho, MR=7401726783en_HK
dc.identifier.scopusauthoridMak, AFT=35516744100en_HK
dc.identifier.scopusauthoridLi, JS=7410064873en_HK
dc.identifier.scopusauthoridWang, M=15749714100en_HK
dc.identifier.scopusauthoridSun, S=55178691300en_HK

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats