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Article: Stability of infectious recombinant adeno-associated viral vector in gene delivery

TitleStability of infectious recombinant adeno-associated viral vector in gene delivery
Authors
KeywordsAAV stocks
Clinical trial
Stability
Transduction efficiency
Issue Date2005
PublisherMedical Science International Publishing. The Journal's web site is located at http://www.medscimonit.com
Citation
Medical Science Monitor, 2005, v. 11 n. 9, p. BR305-BR308 How to Cite?
AbstractBackground: The aim of this study is to provide a basis for the design of appropriate protocols for the shipping and storage of rAAV vectors for experimental laboratory studies and clinical trials. Material/Methods: rAAV stocks were generated by standard methods and then subjected to different environments. The transduction efficiency of viral vectors both in vitro and in vivo was determined by luciferase activity and immunohistochemistry. Results: The virus stored at -80°C remained completely stable and had high transduction efficiency. By contrast, the transduction efficiency of all other groups on 293 cells decreased continuously over time. The transduction efficiency of the -20°C group remained relatively high for the first 5 days, but dropped sharply between days 5 and 7. The transduction efficiency for the 4°C group dropped sharply on both days 1 and 7, and continued to decrease to 55% of maximum efficiency by the end of the first month. For both the room temperature (RT) and 37°C groups, a sharp fall in efficiency was observed at day 1, and efficiency continued to decline throughout the experimental period. Data from the in vivo study also revealed that rAAV vector stored at -80°C remained stable and retained its transduction efficiency. Conclusions: The virus stored at -80°C remained completely stable and retained high transduction efficiency. The implications of these findings provide a basis for viral stock portioning and avoidance of freeze-thawing and storing at temperatures above -80°C prior to clinical trials. © Med Sci Monit, 2005.
Persistent Identifierhttp://hdl.handle.net/10722/74097
ISSN
2012 Impact Factor: 1.358
2015 SCImago Journal Rankings: 0.512
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorXu, Ren_HK
dc.contributor.authorRahimi, Men_HK
dc.contributor.authorMa, Hen_HK
dc.contributor.authorFung, Pen_HK
dc.contributor.authorChang, Cen_HK
dc.contributor.authorXu, Sen_HK
dc.contributor.authorDuring, Men_HK
dc.date.accessioned2010-09-06T06:57:46Z-
dc.date.available2010-09-06T06:57:46Z-
dc.date.issued2005en_HK
dc.identifier.citationMedical Science Monitor, 2005, v. 11 n. 9, p. BR305-BR308en_HK
dc.identifier.issn1234-1010en_HK
dc.identifier.urihttp://hdl.handle.net/10722/74097-
dc.description.abstractBackground: The aim of this study is to provide a basis for the design of appropriate protocols for the shipping and storage of rAAV vectors for experimental laboratory studies and clinical trials. Material/Methods: rAAV stocks were generated by standard methods and then subjected to different environments. The transduction efficiency of viral vectors both in vitro and in vivo was determined by luciferase activity and immunohistochemistry. Results: The virus stored at -80°C remained completely stable and had high transduction efficiency. By contrast, the transduction efficiency of all other groups on 293 cells decreased continuously over time. The transduction efficiency of the -20°C group remained relatively high for the first 5 days, but dropped sharply between days 5 and 7. The transduction efficiency for the 4°C group dropped sharply on both days 1 and 7, and continued to decrease to 55% of maximum efficiency by the end of the first month. For both the room temperature (RT) and 37°C groups, a sharp fall in efficiency was observed at day 1, and efficiency continued to decline throughout the experimental period. Data from the in vivo study also revealed that rAAV vector stored at -80°C remained stable and retained its transduction efficiency. Conclusions: The virus stored at -80°C remained completely stable and retained high transduction efficiency. The implications of these findings provide a basis for viral stock portioning and avoidance of freeze-thawing and storing at temperatures above -80°C prior to clinical trials. © Med Sci Monit, 2005.en_HK
dc.languageengen_HK
dc.publisherMedical Science International Publishing. The Journal's web site is located at http://www.medscimonit.comen_HK
dc.relation.ispartofMedical Science Monitoren_HK
dc.subjectAAV stocksen_HK
dc.subjectClinical trialen_HK
dc.subjectStabilityen_HK
dc.subjectTransduction efficiencyen_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshCell Lineen_HK
dc.subject.meshCryopreservationen_HK
dc.subject.meshDependovirus - geneticsen_HK
dc.subject.meshGene Therapyen_HK
dc.subject.meshGenetic Vectorsen_HK
dc.subject.meshHumansen_HK
dc.subject.meshLuciferases - geneticsen_HK
dc.subject.meshMaleen_HK
dc.subject.meshRatsen_HK
dc.subject.meshRats, Wistaren_HK
dc.subject.meshRecombinant Proteins - geneticsen_HK
dc.subject.meshRecombination, Geneticen_HK
dc.subject.meshTemperatureen_HK
dc.subject.meshTransduction, Geneticen_HK
dc.titleStability of infectious recombinant adeno-associated viral vector in gene deliveryen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1234-1010&volume=11&spage=305&epage=308&date=2005&atitle=Stability+of+infectious+recombinant+adeno-associated+viral+vector+in+gene+deliveryen_HK
dc.identifier.emailChang, C: cqchang@eee.hku.hken_HK
dc.identifier.authorityChang, C=rp00095en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.pmid16127351-
dc.identifier.scopuseid_2-s2.0-24944556630en_HK
dc.identifier.hkuros105800en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-24944556630&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume11en_HK
dc.identifier.issue9en_HK
dc.identifier.spageBR305en_HK
dc.identifier.epageBR308en_HK
dc.identifier.isiWOS:000231928500002-
dc.publisher.placePolanden_HK
dc.identifier.scopusauthoridXu, R=7402813857en_HK
dc.identifier.scopusauthoridRahimi, M=8930560500en_HK
dc.identifier.scopusauthoridMa, H=8934492600en_HK
dc.identifier.scopusauthoridFung, P=7101613315en_HK
dc.identifier.scopusauthoridChang, C=7407033052en_HK
dc.identifier.scopusauthoridXu, S=7404439649en_HK
dc.identifier.scopusauthoridDuring, M=7005720022en_HK

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