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Article: A comparative ESR study on blood and tissue nitric oxide concentration during renal ischemia-reperfusion injury

TitleA comparative ESR study on blood and tissue nitric oxide concentration during renal ischemia-reperfusion injury
Authors
Issue Date2007
PublisherSpringer-Verlag Wien. The Journal's web site is located at http://www.springer.at/amr
Citation
Applied Magnetic Resonance, 2007, v. 32 n. 3, p. 243-255 How to Cite?
AbstractElectron paramagnetic resonance (EPR) spin trapping technology is a sensitive and unambiguous method for detection of nitric oxide (NO). Due to the short lifetime, NO must be trapped before EPR measurement. There are two EPR spin trapping techniques used currently, including the detections of EPR signals of diethyldithiocarbamate-iron-nitric oxide (DETC 2-Fe 2+-NO) and nitrosyl hemoglobin (HbNO). In this study, we firstly investigated the kinetics of the EPR signal of DETC 2-Fe 2+-NO in normal and ischemia-reperfused kidneys. In normal rat kidneys, the signal of DETC 2-Fe 2+-NO was found at 5 min after the spin trappers Fe 2+/DETC were administrated, the peak concentration was at 15 min and the period with relatively stable signal intensity was at the time range from 15 to 70 min. In the ischemia-reperfused rat kidneys, the signal of DETC 2-Fe 2+-NO was increased at 30 min of ischemia and decreased at 60 min of ischemia after the occlusion of renal artery (corresponding to the time course of 60 and 90 min after Fe 2+/DETC injection respectively). We then investigated the EPR signal of HbNO in blood. No characteristic HbNO signal was found in the rats of the sham control and 30 min of ischemia. An HbNO signal occurred in the rats exposed to 60 min of ischemia and it became pronounced with increased duration of reperfusion. The signal intensity reached a plateau at 150 min of reperfusion. The results suggest that the DETC 2-Fe 2+-NO signal can be only suitable for the NO measurement in the short-term ischemia-reperfusion model, whereas the HbNO signal can be applied to represent NO in the relatively long-term ischemia-reperfusion model. In addition, N G-nitro-L- arginine (L-NAME) and allopurinol were used to identify the source of NO. By detecting the HbNO signal, we demonstrated that the activation of xanthine oxidase is an important source of NO formation at the long-term period of ischemia and reperfusion. © Springer-Verlag 2007.
Persistent Identifierhttp://hdl.handle.net/10722/73717
ISSN
2015 Impact Factor: 0.884
2015 SCImago Journal Rankings: 0.358
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorRen, Jen_HK
dc.contributor.authorFung, PCWen_HK
dc.contributor.authorChang, Cen_HK
dc.contributor.authorShen, GXen_HK
dc.contributor.authorLu, Gen_HK
dc.contributor.authorChan, FHYen_HK
dc.contributor.authorLiu, KJen_HK
dc.contributor.authorShen, Jen_HK
dc.date.accessioned2010-09-06T06:54:05Z-
dc.date.available2010-09-06T06:54:05Z-
dc.date.issued2007en_HK
dc.identifier.citationApplied Magnetic Resonance, 2007, v. 32 n. 3, p. 243-255en_HK
dc.identifier.issn0937-9347en_HK
dc.identifier.urihttp://hdl.handle.net/10722/73717-
dc.description.abstractElectron paramagnetic resonance (EPR) spin trapping technology is a sensitive and unambiguous method for detection of nitric oxide (NO). Due to the short lifetime, NO must be trapped before EPR measurement. There are two EPR spin trapping techniques used currently, including the detections of EPR signals of diethyldithiocarbamate-iron-nitric oxide (DETC 2-Fe 2+-NO) and nitrosyl hemoglobin (HbNO). In this study, we firstly investigated the kinetics of the EPR signal of DETC 2-Fe 2+-NO in normal and ischemia-reperfused kidneys. In normal rat kidneys, the signal of DETC 2-Fe 2+-NO was found at 5 min after the spin trappers Fe 2+/DETC were administrated, the peak concentration was at 15 min and the period with relatively stable signal intensity was at the time range from 15 to 70 min. In the ischemia-reperfused rat kidneys, the signal of DETC 2-Fe 2+-NO was increased at 30 min of ischemia and decreased at 60 min of ischemia after the occlusion of renal artery (corresponding to the time course of 60 and 90 min after Fe 2+/DETC injection respectively). We then investigated the EPR signal of HbNO in blood. No characteristic HbNO signal was found in the rats of the sham control and 30 min of ischemia. An HbNO signal occurred in the rats exposed to 60 min of ischemia and it became pronounced with increased duration of reperfusion. The signal intensity reached a plateau at 150 min of reperfusion. The results suggest that the DETC 2-Fe 2+-NO signal can be only suitable for the NO measurement in the short-term ischemia-reperfusion model, whereas the HbNO signal can be applied to represent NO in the relatively long-term ischemia-reperfusion model. In addition, N G-nitro-L- arginine (L-NAME) and allopurinol were used to identify the source of NO. By detecting the HbNO signal, we demonstrated that the activation of xanthine oxidase is an important source of NO formation at the long-term period of ischemia and reperfusion. © Springer-Verlag 2007.en_HK
dc.languageengen_HK
dc.publisherSpringer-Verlag Wien. The Journal's web site is located at http://www.springer.at/amren_HK
dc.relation.ispartofApplied Magnetic Resonanceen_HK
dc.titleA comparative ESR study on blood and tissue nitric oxide concentration during renal ischemia-reperfusion injuryen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0937-9347&volume=32&spage=243&epage=255&date=2007&atitle=A+comparative+ESR+study+on+blood+and+tissue+nitric+oxide+concentration+during+renal+ischemia-reperfusion+injury+en_HK
dc.identifier.emailChang, C: cqchang@eee.hku.hken_HK
dc.identifier.emailShen, GX: gxshen@eee.hku.hken_HK
dc.identifier.emailShen, J: shenjg@hku.hken_HK
dc.identifier.authorityChang, C=rp00095en_HK
dc.identifier.authorityShen, GX=rp00166en_HK
dc.identifier.authorityShen, J=rp00487en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1007/s00723-007-0024-1en_HK
dc.identifier.scopuseid_2-s2.0-47849125651en_HK
dc.identifier.hkuros140228en_HK
dc.identifier.hkuros137364-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-47849125651&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume32en_HK
dc.identifier.issue3en_HK
dc.identifier.spage243en_HK
dc.identifier.epage255en_HK
dc.identifier.isiWOS:000251837000001-
dc.publisher.placeAustriaen_HK
dc.identifier.scopusauthoridRen, J=7403083223en_HK
dc.identifier.scopusauthoridFung, PCW=7101613315en_HK
dc.identifier.scopusauthoridChang, C=7407033052en_HK
dc.identifier.scopusauthoridShen, GX=7401967224en_HK
dc.identifier.scopusauthoridLu, G=24476428000en_HK
dc.identifier.scopusauthoridChan, FHY=7202586429en_HK
dc.identifier.scopusauthoridLiu, KJ=7404200456en_HK
dc.identifier.scopusauthoridShen, J=7404929947en_HK

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