Transcription of mnp gene in inter-kingdom fusant Fhhh for degradation of PTA wastewater

Abstract

The molecular genetic stability of the inter- kingdom fusant Fhhh and the transcription of mnp gene in degradation of the purified terephthalic acid (PTA) wastewater were studied in this paper. Fhhh contained 3 DNA fragments including mnp gene , FLOI gene and 16SrDNA demonstrated with polymerase chain reaction. The 3 DNA fragments were from the three parental strains , which were the fungous Phanerochaete chrysosporium , the fungous S accharomyces cerevisiae , and the native bacterium strain YZI respectively. The transcription of mnp gene existing in both Fhhh and P chrysosporium could happen meaured with RT- PCR in degradation of PTA wastewater. Meanwhile 4 metal ions of Mn2 + , Cu2 + , Zn2 + and Se4 + in PTA wastewater were at optimal levels. The data suggested that the fact of mnp transcription of Fhhh in PTA wastewater should be favorable for setting up regulating system to increase treatment efficiency in molecular genetics. 報道Fhhh的遺傳穩定性, 及其降解精對苯二甲酸(PTA)廢水過程中基因轉錄的研究結果. 聚合酶鏈式反應PCR的測定結果, Fhhh同時含有mnp(來自黃孢原毛平革真菌)、FL01(來自釀酒酵母真菌)和16SrDNA(來自土著細菌)的3個親株的基因DNA片斷. 表明Fhhh具有分子遺傳穩定性. 反轉錄-擴增(RT_PCR)的測定表明, Fhhh和黃孢原毛平革菌在降解PTA廢水過程中, 在Mn2+、Cu2+、Zn2+、Se4+ 4種金屬元素優化水平的條件下, mnp基因均可轉錄. 研究結果為高效處理PTA廢水, 增添了分子遺傳學的調控途徑.