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Article: Biochemical pathway and degradation of phthalate ester isomers by bacteria

TitleBiochemical pathway and degradation of phthalate ester isomers by bacteria
Authors
KeywordsBiochemical cooperation
Degradation
Phthalate ester
Plasticizer
Wetland
Issue Date2005
PublisherI W A Publishing. The Journal's web site is located at http://www.iwapublishing.com/template.cfm?name=iwapwst
Citation
Water Science And Technology, 2005, v. 52 n. 8, p. 241-248 How to Cite?
AbstractDegradation of dimethyl isophthalate (DMI) and dimethyl phthalate ester (DMPE) was investigated using microorganisms isolated from mangrove sediment of Hong Kong Mai Po Nature Reserve. One enrichment culture was capable of utilizing DMI as the sole source of carbon and energy, but none of the bacteria in the enrichment culture was capable of degrading DMI alone. In co-culture of two bacteria, degradation was observed proceeding through monomethyl isophthalate (MMI) ester and isophthalic acid (IPA) before the aromatic ring opening. Using DMI as the sole carbon and energy source, Klebsiella oxytoca Sc and Methylobacterium mesophilicum Sr degraded DMI through the biochemical cooperation. The initial hydrolytic reaction of the ester bond was by K. oxytoca Sc and the next step of transformation was by M. mesophilicum Sr, and IPA was degraded by both of them. In another investigation, a novel bacterium, strain MPsc, was isolated for degradation of dimethyl phthalate ester (DMPE) also from the mangrove sediment. On the basis of phenotypic, biochemical and 16S rDNA gene sequence analyses, the strain MPsc should be considered as a new bacterium at the genus level (8% differences). This strain, together with a Rhodococcus zopfii isolated from the same mangrove sediment, was able to degrade DMPE aerobically. The consortium consisting of the two species degraded 450 mg/l DMPE within 3 days as the sole source of carbon and energy, but none of the individual species alone was able to transform DMPE. Furthermore, the biochemical degradation pathway proceeded through monomethyl phthalate (MMP), phthalic acid (PA) and then protocatechuate before aromatic ring cleavage. Our results suggest that degradation of complex organic compounds including DMI and DMPE may be carried out by several members of microorganisms working together in the natural environments. © IWA Publishing 2005.
Persistent Identifierhttp://hdl.handle.net/10722/73415
ISSN
2015 Impact Factor: 1.064
2015 SCImago Journal Rankings: 0.469
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorGu, JDen_HK
dc.contributor.authorLi, Jen_HK
dc.contributor.authorWang, Yen_HK
dc.date.accessioned2010-09-06T06:51:03Z-
dc.date.available2010-09-06T06:51:03Z-
dc.date.issued2005en_HK
dc.identifier.citationWater Science And Technology, 2005, v. 52 n. 8, p. 241-248en_HK
dc.identifier.issn0273-1223en_HK
dc.identifier.urihttp://hdl.handle.net/10722/73415-
dc.description.abstractDegradation of dimethyl isophthalate (DMI) and dimethyl phthalate ester (DMPE) was investigated using microorganisms isolated from mangrove sediment of Hong Kong Mai Po Nature Reserve. One enrichment culture was capable of utilizing DMI as the sole source of carbon and energy, but none of the bacteria in the enrichment culture was capable of degrading DMI alone. In co-culture of two bacteria, degradation was observed proceeding through monomethyl isophthalate (MMI) ester and isophthalic acid (IPA) before the aromatic ring opening. Using DMI as the sole carbon and energy source, Klebsiella oxytoca Sc and Methylobacterium mesophilicum Sr degraded DMI through the biochemical cooperation. The initial hydrolytic reaction of the ester bond was by K. oxytoca Sc and the next step of transformation was by M. mesophilicum Sr, and IPA was degraded by both of them. In another investigation, a novel bacterium, strain MPsc, was isolated for degradation of dimethyl phthalate ester (DMPE) also from the mangrove sediment. On the basis of phenotypic, biochemical and 16S rDNA gene sequence analyses, the strain MPsc should be considered as a new bacterium at the genus level (8% differences). This strain, together with a Rhodococcus zopfii isolated from the same mangrove sediment, was able to degrade DMPE aerobically. The consortium consisting of the two species degraded 450 mg/l DMPE within 3 days as the sole source of carbon and energy, but none of the individual species alone was able to transform DMPE. Furthermore, the biochemical degradation pathway proceeded through monomethyl phthalate (MMP), phthalic acid (PA) and then protocatechuate before aromatic ring cleavage. Our results suggest that degradation of complex organic compounds including DMI and DMPE may be carried out by several members of microorganisms working together in the natural environments. © IWA Publishing 2005.en_HK
dc.languageengen_HK
dc.publisherI W A Publishing. The Journal's web site is located at http://www.iwapublishing.com/template.cfm?name=iwapwsten_HK
dc.relation.ispartofWater Science and Technologyen_HK
dc.subjectBiochemical cooperationen_HK
dc.subjectDegradationen_HK
dc.subjectPhthalate esteren_HK
dc.subjectPlasticizeren_HK
dc.subjectWetlanden_HK
dc.titleBiochemical pathway and degradation of phthalate ester isomers by bacteriaen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0273-1223&volume=52&issue=8&spage=241&epage=248&date=2005&atitle=Biochemical+pathway+and+degradation+of+phthalate+ester+isomers+by+bacteriaen_HK
dc.identifier.emailGu, JD: jdgu@hkucc.hku.hken_HK
dc.identifier.authorityGu, JD=rp00701en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.pmid16312973-
dc.identifier.scopuseid_2-s2.0-28444449404en_HK
dc.identifier.hkuros116761en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-28444449404&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume52en_HK
dc.identifier.issue8en_HK
dc.identifier.spage241en_HK
dc.identifier.epage248en_HK
dc.identifier.isiWOS:000233644300029-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridGu, JD=7403129601en_HK
dc.identifier.scopusauthoridLi, J=26643007400en_HK
dc.identifier.scopusauthoridWang, Y=9736272200en_HK

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