File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: pVC, a small cryptic plasmid from the environmental isolate of Vibrio cholerae MP-1

TitlepVC, a small cryptic plasmid from the environmental isolate of Vibrio cholerae MP-1
Authors
KeywordsHorizontal gene transfer
Plasmid
Replicon
Vibrio cholerae
Issue Date2007
PublisherHan-Gug Misaengmul Hag-hoe. The Journal's web site is located at http://www.msk.or.kr/jm/jmhome.htm
Citation
Journal Of Microbiology, 2007, v. 45 n. 3, p. 193-198 How to Cite?
AbstractA marine bacterium was isolated from Mai Po Nature Reserve of Hong Kong and identified as Vibrio cholerae MP-1. It contains a small plasmid designated as pVC of 3.8 kb. Four open reading frames (ORFs) are identified on the plasmid, but none of them shows homology to any known protein. Database search indicated that a 440 bp fragment is 96% identical to a fragment found in a small plasmid of another V. cholerae. Further experiments demonstrated that a 2.3 kb EcoRI fragment containing the complete ORF1, partial ORF4 and their intergenic region could self-replicate. Additional analyses revealed that sequence upstream of ORF1 showed the features characteristic of theta type replicons. Protein encoded by ORF1 has two characteristic motifs existed in most replication initiator proteins (Rep): the leucine zipper (LZ) motif located at the N-terminal region and the alpha helix-turn-alpha helix motif (HTH) located at the C-terminal end. The results suggest that pVC replicates via the theta type mechanism and is likely a novel type of theta replicon. Copyright © 2007, The Microbiological Society of Korea.
Persistent Identifierhttp://hdl.handle.net/10722/73377
ISSN
2015 Impact Factor: 1.621
2015 SCImago Journal Rankings: 0.741
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorZhang, Ren_HK
dc.contributor.authorWang, Yen_HK
dc.contributor.authorPak, CLen_HK
dc.contributor.authorGu, JDen_HK
dc.date.accessioned2010-09-06T06:50:42Z-
dc.date.available2010-09-06T06:50:42Z-
dc.date.issued2007en_HK
dc.identifier.citationJournal Of Microbiology, 2007, v. 45 n. 3, p. 193-198en_HK
dc.identifier.issn1225-8873en_HK
dc.identifier.urihttp://hdl.handle.net/10722/73377-
dc.description.abstractA marine bacterium was isolated from Mai Po Nature Reserve of Hong Kong and identified as Vibrio cholerae MP-1. It contains a small plasmid designated as pVC of 3.8 kb. Four open reading frames (ORFs) are identified on the plasmid, but none of them shows homology to any known protein. Database search indicated that a 440 bp fragment is 96% identical to a fragment found in a small plasmid of another V. cholerae. Further experiments demonstrated that a 2.3 kb EcoRI fragment containing the complete ORF1, partial ORF4 and their intergenic region could self-replicate. Additional analyses revealed that sequence upstream of ORF1 showed the features characteristic of theta type replicons. Protein encoded by ORF1 has two characteristic motifs existed in most replication initiator proteins (Rep): the leucine zipper (LZ) motif located at the N-terminal region and the alpha helix-turn-alpha helix motif (HTH) located at the C-terminal end. The results suggest that pVC replicates via the theta type mechanism and is likely a novel type of theta replicon. Copyright © 2007, The Microbiological Society of Korea.en_HK
dc.languageengen_HK
dc.publisherHan-Gug Misaengmul Hag-hoe. The Journal's web site is located at http://www.msk.or.kr/jm/jmhome.htmen_HK
dc.relation.ispartofJournal of Microbiologyen_HK
dc.subjectHorizontal gene transferen_HK
dc.subjectPlasmiden_HK
dc.subjectRepliconen_HK
dc.subjectVibrio choleraeen_HK
dc.titlepVC, a small cryptic plasmid from the environmental isolate of Vibrio cholerae MP-1en_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1225-8873&volume=45&spage=193&epage=198&date=2007&atitle=pVC,+a+small+cryptic+plasmid+from+the+environmental+isolate+of+Vibrio+cholerae+MP-1en_HK
dc.identifier.emailPak, CL: pcleung@hkucc.hku.hken_HK
dc.identifier.emailGu, JD: jdgu@hkucc.hku.hken_HK
dc.identifier.authorityPak, CL=rp00735en_HK
dc.identifier.authorityGu, JD=rp00701en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.pmid17618223en_HK
dc.identifier.scopuseid_2-s2.0-34447133466en_HK
dc.identifier.hkuros134282en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-34447133466&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume45en_HK
dc.identifier.issue3en_HK
dc.identifier.spage193en_HK
dc.identifier.epage198en_HK
dc.identifier.isiWOS:000247703700002-
dc.publisher.placeKorea, Republic ofen_HK
dc.identifier.scopusauthoridZhang, R=8612262700en_HK
dc.identifier.scopusauthoridWang, Y=12776188600en_HK
dc.identifier.scopusauthoridPak, CL=35080988800en_HK
dc.identifier.scopusauthoridGu, JD=7403129601en_HK

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats