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Article: Screening of basidiomycetes and xylariaceous fungi for lignin peroxidase and laccase gene-specific sequences

TitleScreening of basidiomycetes and xylariaceous fungi for lignin peroxidase and laccase gene-specific sequences
Authors
Issue Date2005
PublisherElsevier Ltd.
Citation
Mycological Research, 2005, v. 109 n. 1, p. 115-124 How to Cite?
AbstractLignin peroxidase and laccase gene-specific PCR primers were used to screen 38 diverse basidiomycetes and xylariaceous fungi. Lignin peroxidase gene-specific sequences were obtained for basidiomycetes only and were highly divergent. Possession of laccase genes was relatively widespread among basidiomycetes, and is shown for the first time in Xylariaceae. All sequences were highly conserved with no variation resulting in changes to predicted amino acid sequence. Those basidiomycetes shown to possess lignin peroxidase and laccase genes also produced the enzyme in vitro. Conversely none of the xylariaceous fungi shown to possess laccase genes were able to do so, whilst others decolorized Poly R yet yielded no PCR amplicons.
Persistent Identifierhttp://hdl.handle.net/10722/73284
ISSN
2011 Impact Factor: 2.809
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorPointing, SBen_HK
dc.contributor.authorPelling, ALen_HK
dc.contributor.authorSmith, GJDen_HK
dc.contributor.authorHyde, KDen_HK
dc.contributor.authorReddy, CAen_HK
dc.date.accessioned2010-09-06T06:49:51Z-
dc.date.available2010-09-06T06:49:51Z-
dc.date.issued2005en_HK
dc.identifier.citationMycological Research, 2005, v. 109 n. 1, p. 115-124en_HK
dc.identifier.issn0953-7562en_HK
dc.identifier.urihttp://hdl.handle.net/10722/73284-
dc.description.abstractLignin peroxidase and laccase gene-specific PCR primers were used to screen 38 diverse basidiomycetes and xylariaceous fungi. Lignin peroxidase gene-specific sequences were obtained for basidiomycetes only and were highly divergent. Possession of laccase genes was relatively widespread among basidiomycetes, and is shown for the first time in Xylariaceae. All sequences were highly conserved with no variation resulting in changes to predicted amino acid sequence. Those basidiomycetes shown to possess lignin peroxidase and laccase genes also produced the enzyme in vitro. Conversely none of the xylariaceous fungi shown to possess laccase genes were able to do so, whilst others decolorized Poly R yet yielded no PCR amplicons.en_HK
dc.languageengen_HK
dc.publisherElsevier Ltd.en_HK
dc.relation.ispartofMycological Researchen_HK
dc.rightsMycological Research. Copyright © Elsevier Ltd.en_HK
dc.subject.meshAmino Acid Sequenceen_HK
dc.subject.meshAscomycota - enzymology - geneticsen_HK
dc.subject.meshBasidiomycota - enzymology - geneticsen_HK
dc.subject.meshCloning, Molecularen_HK
dc.subject.meshLaccase - genetics - metabolismen_HK
dc.subject.meshMolecular Sequence Dataen_HK
dc.subject.meshPeroxidases - genetics - metabolismen_HK
dc.subject.meshPhylogenyen_HK
dc.subject.meshPolymerase Chain Reactionen_HK
dc.subject.meshSequence Alignmenten_HK
dc.subject.meshSequence Analysis, DNAen_HK
dc.titleScreening of basidiomycetes and xylariaceous fungi for lignin peroxidase and laccase gene-specific sequencesen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0953-7562&volume=109&spage=115&epage=124&date=2005&atitle=Screening+of+basidiomycetes+and+xylariaceous+fungi+for+lignin+peroxidase+and+laccase+gene-specific+sequencesen_HK
dc.identifier.emailPointing, SB: pointing@hkucc.hku.hken_HK
dc.identifier.emailSmith, GJD: gjsmith@hkucc.hku.hken_HK
dc.identifier.emailHyde, KD: kdhyde@hkucc.hku.hk-
dc.identifier.authorityPointing, SB=rp00771en_HK
dc.identifier.authoritySmith, GJD=rp00444en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1017/S0953756204001376en_HK
dc.identifier.pmid15736869en_HK
dc.identifier.scopuseid_2-s2.0-13544249793en_HK
dc.identifier.hkuros113714en_HK
dc.identifier.hkuros115564-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-13544249793&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume109en_HK
dc.identifier.issue1en_HK
dc.identifier.spage115en_HK
dc.identifier.epage124en_HK
dc.identifier.isiWOS:000227421200011-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridPointing, SB=6603986412en_HK
dc.identifier.scopusauthoridPelling, AL=6602962713en_HK
dc.identifier.scopusauthoridSmith, GJD=8344015800en_HK
dc.identifier.scopusauthoridHyde, KD=7102588111en_HK
dc.identifier.scopusauthoridReddy, CA=55416903900en_HK

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