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Conference Paper: Time should be considered in developmental ecotoxicity test

TitleTime should be considered in developmental ecotoxicity test
Authors
Issue Date2002
PublisherPergamon. The Journal's web site is located at http://www.elsevier.com/locate/marpolbul
Citation
The 3ird International Conference on Marine Pollution and Ecotoxicology, Hong Kong, 10–14th June 2001. In Marine Pollution Bulletin, 2002, v. 45 n. 1-12, p. 92-99 How to Cite?
AbstractDevelopmental toxicity tests are often used for the hazard assessment of chemicals and environmental media. One of the most widely used is the oyster embryo larval test (OEL), in which the development of oyster larvae is arrested at a single fixed time (e.g. 24 or 48 h) of toxic exposure, and the proportion of normal larvae measured. However, a major problem with this conventional approach is the lack of information on temporal trends in development. In this study, Pacific oyster Crassostrea gigas embryos were exposed to nominal concentrations of copper (CuSO 4) of <0.001 (control), 0.60, 1.25, 2.5, 5.0, 10.0 and 20.0 μgl -1 (at 20°C, salinity 35‰ and pH 8.1). Three replicates from each group were arrested and examined every 8 h during 24-72 h of exposure, and the number of viable larvae developed to D-shape was determined. The results revealed that the number of viable D-shape larvae in the control increased rapidly and reached an optimum at 32 h, before declining gradually due to starvation. Analysis of covariance (ANCOVA) showed that larval developmental rates during 0-32 h were significantly inhibited by Cu at all concentrations. This paper demonstrates that arrest and measurement at different time periods are important and should be incorporated into the OEL test. This would maximise the sensitivity of the test in detecting developmental effects in spiked or environmental samples. © 2002 Elsevier Science Ltd. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/73249
ISSN
2015 Impact Factor: 3.099
2015 SCImago Journal Rankings: 1.264
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorWorboys, MAen_HK
dc.contributor.authorLeung, KMYen_HK
dc.contributor.authorGrist, EPMen_HK
dc.contributor.authorCrane, Men_HK
dc.date.accessioned2010-09-06T06:49:32Z-
dc.date.available2010-09-06T06:49:32Z-
dc.date.issued2002en_HK
dc.identifier.citationThe 3ird International Conference on Marine Pollution and Ecotoxicology, Hong Kong, 10–14th June 2001. In Marine Pollution Bulletin, 2002, v. 45 n. 1-12, p. 92-99en_HK
dc.identifier.issn0025-326Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/73249-
dc.description.abstractDevelopmental toxicity tests are often used for the hazard assessment of chemicals and environmental media. One of the most widely used is the oyster embryo larval test (OEL), in which the development of oyster larvae is arrested at a single fixed time (e.g. 24 or 48 h) of toxic exposure, and the proportion of normal larvae measured. However, a major problem with this conventional approach is the lack of information on temporal trends in development. In this study, Pacific oyster Crassostrea gigas embryos were exposed to nominal concentrations of copper (CuSO 4) of <0.001 (control), 0.60, 1.25, 2.5, 5.0, 10.0 and 20.0 μgl -1 (at 20°C, salinity 35‰ and pH 8.1). Three replicates from each group were arrested and examined every 8 h during 24-72 h of exposure, and the number of viable larvae developed to D-shape was determined. The results revealed that the number of viable D-shape larvae in the control increased rapidly and reached an optimum at 32 h, before declining gradually due to starvation. Analysis of covariance (ANCOVA) showed that larval developmental rates during 0-32 h were significantly inhibited by Cu at all concentrations. This paper demonstrates that arrest and measurement at different time periods are important and should be incorporated into the OEL test. This would maximise the sensitivity of the test in detecting developmental effects in spiked or environmental samples. © 2002 Elsevier Science Ltd. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherPergamon. The Journal's web site is located at http://www.elsevier.com/locate/marpolbulen_HK
dc.relation.ispartofMarine Pollution Bulletinen_HK
dc.titleTime should be considered in developmental ecotoxicity testen_HK
dc.typeConference_Paperen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0025-326X&volume=45&spage=92&epage=99&date=2002&atitle=Time+should+be+considered+in+developmental+ecotoxicity+testsen_HK
dc.identifier.emailLeung, KMY: kmyleung@hku.hken_HK
dc.identifier.authorityLeung, KMY=rp00733en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/S0025-326X(02)00116-9en_HK
dc.identifier.pmid12398372-
dc.identifier.scopuseid_2-s2.0-0036741525en_HK
dc.identifier.hkuros76102en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0036741525&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume45en_HK
dc.identifier.issue1-12en_HK
dc.identifier.spage92en_HK
dc.identifier.epage99en_HK
dc.identifier.isiWOS:000178336300012-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridWorboys, MA=7004466579en_HK
dc.identifier.scopusauthoridLeung, KMY=7401860738en_HK
dc.identifier.scopusauthoridGrist, EPM=7003398590en_HK
dc.identifier.scopusauthoridCrane, M=7202466944en_HK
dc.customcontrol.immutablesml 151013 - merged-

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