File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Membrane-associated hexavalent chromium reductase of Bacillus megaterium TKW3 with induced expression

TitleMembrane-associated hexavalent chromium reductase of Bacillus megaterium TKW3 with induced expression
Authors
KeywordsBacillus megaterium
Bioremediation
Chromate reductase
Metalloid
Proteomics
Selenium
Issue Date2006
PublisherHan'gug Mi'saengmul Saengmyeong Gong Haghoe. The Journal's web site is located at http://www.jmb.or.kr/
Citation
Journal Of Microbiology And Biotechnology, 2006, v. 16 n. 6, p. 855-862 How to Cite?
AbstractHexavalent chromium (Cr6+) is a highly harmful pollutant, which can be detoxified and precipitated through reduction to Cr3+. Bacillus megaterium TKW3 previously isolated from chromium-contaminated marine sediments was capable of reducing Cr6+ in concomitance with metalloids (Se4+, Se6+, and As5+). Notwithstanding approximately 50% inhibition, it was the first report of simultaneous bacterial reduction of Cr6+ and Se4+ (to elemental Se). No significant difference was observed among electron donors (glucose, maltose, and mannitol) on Cr6+ reduction by B. megaterium TKW3. The reduction was constitutive and determined to be non-plasmid mediated. Peptide mass fingerprints (PMF) revealed a novel aerobic membrane-associated reductase with Cr6+-induced expression and specific reductive activity (in nmol Cr6+/mg protein/min) of 0.220 as compared with 0.087 of the soluble protein fraction. Respiratory inhibitor NaN3 did not interfere with the reductase activity. Transmission electron microscopy with energy dispersive X-ray (TEM-EDX) analysis confirmed the aggregation of reduced chromium along the intracellular membrane region. Future identification of the N-terminal amino acid sequence of this reductase will facilitate purification and understanding of its enzymatic action. © The Korean Society for Microbiology and Biotechnology.
Persistent Identifierhttp://hdl.handle.net/10722/73246
ISSN
2015 Impact Factor: 1.685
2015 SCImago Journal Rankings: 0.548
References

 

DC FieldValueLanguage
dc.contributor.authorCheung, KHen_HK
dc.contributor.authorLai, HYen_HK
dc.contributor.authorGu, JDen_HK
dc.date.accessioned2010-09-06T06:49:30Z-
dc.date.available2010-09-06T06:49:30Z-
dc.date.issued2006en_HK
dc.identifier.citationJournal Of Microbiology And Biotechnology, 2006, v. 16 n. 6, p. 855-862en_HK
dc.identifier.issn1017-7825en_HK
dc.identifier.urihttp://hdl.handle.net/10722/73246-
dc.description.abstractHexavalent chromium (Cr6+) is a highly harmful pollutant, which can be detoxified and precipitated through reduction to Cr3+. Bacillus megaterium TKW3 previously isolated from chromium-contaminated marine sediments was capable of reducing Cr6+ in concomitance with metalloids (Se4+, Se6+, and As5+). Notwithstanding approximately 50% inhibition, it was the first report of simultaneous bacterial reduction of Cr6+ and Se4+ (to elemental Se). No significant difference was observed among electron donors (glucose, maltose, and mannitol) on Cr6+ reduction by B. megaterium TKW3. The reduction was constitutive and determined to be non-plasmid mediated. Peptide mass fingerprints (PMF) revealed a novel aerobic membrane-associated reductase with Cr6+-induced expression and specific reductive activity (in nmol Cr6+/mg protein/min) of 0.220 as compared with 0.087 of the soluble protein fraction. Respiratory inhibitor NaN3 did not interfere with the reductase activity. Transmission electron microscopy with energy dispersive X-ray (TEM-EDX) analysis confirmed the aggregation of reduced chromium along the intracellular membrane region. Future identification of the N-terminal amino acid sequence of this reductase will facilitate purification and understanding of its enzymatic action. © The Korean Society for Microbiology and Biotechnology.en_HK
dc.languageengen_HK
dc.publisherHan'gug Mi'saengmul Saengmyeong Gong Haghoe. The Journal's web site is located at http://www.jmb.or.kr/en_HK
dc.relation.ispartofJournal of Microbiology and Biotechnologyen_HK
dc.subjectBacillus megateriumen_HK
dc.subjectBioremediationen_HK
dc.subjectChromate reductaseen_HK
dc.subjectMetalloiden_HK
dc.subjectProteomicsen_HK
dc.subjectSeleniumen_HK
dc.titleMembrane-associated hexavalent chromium reductase of Bacillus megaterium TKW3 with induced expressionen_HK
dc.typeArticleen_HK
dc.identifier.emailGu, JD: jdgu@hkucc.hku.hken_HK
dc.identifier.authorityGu, JD=rp00701en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.scopuseid_2-s2.0-33745617675en_HK
dc.identifier.hkuros119910en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-33745617675&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume16en_HK
dc.identifier.issue6en_HK
dc.identifier.spage855en_HK
dc.identifier.epage862en_HK
dc.publisher.placeKorea, Republic ofen_HK
dc.identifier.scopusauthoridCheung, KH=7402406659en_HK
dc.identifier.scopusauthoridLai, HY=15755593800en_HK
dc.identifier.scopusauthoridGu, JD=7403129601en_HK

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats