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Article: Analysis of parental strain DNA fragments existing in GEMs-Fhhh

TitleAnalysis of parental strain DNA fragments existing in GEMs-Fhhh
Authors
KeywordsDNA fragment
GEMs
PCR
Pollution control
Protoplast fusion
Target gene
Issue Date2003
PublisherI O S Press. The Journal's web site is located at http://www.iospress.nl/html/10010742.php
Citation
Journal Of Environmental Sciences, 2003, v. 15 n. 5, p. 590-594 How to Cite?
AbstractThere were 6 target DNA fragments of the three parental strains existing in the cell of GEMs(genetically engineered microorganism strain) Fhhh measured in this research by PCR(polymerase chain reaction). The determination showed that GEMs Fhhh contained all the 6 target DNA fragments, mnp1, mnp2, lip1, lip2, FLO1 and 16S rDNA, and had the molecular genetic stability. Meanwhile the PCR production of each parental strain could only had its target DNA fragments and was different from each other. It may illustrate that the technique of the inter-kingdom protoplast fusion for the construction of GEMs Fhhh through the process of intercellular gene recombination could be used as a reliable bioengineering technique to create the specific functional stain for the pollution control.
Persistent Identifierhttp://hdl.handle.net/10722/73245
ISSN
2015 Impact Factor: 2.208
2015 SCImago Journal Rankings: 0.832
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorHao, CBen_HK
dc.contributor.authorYan, Jen_HK
dc.contributor.authorQu, MMen_HK
dc.contributor.authorWang, Den_HK
dc.contributor.authorCheng, SPen_HK
dc.contributor.authorGu, JDen_HK
dc.contributor.authorQiu, WFen_HK
dc.contributor.authorWang, YYen_HK
dc.date.accessioned2010-09-06T06:49:30Z-
dc.date.available2010-09-06T06:49:30Z-
dc.date.issued2003en_HK
dc.identifier.citationJournal Of Environmental Sciences, 2003, v. 15 n. 5, p. 590-594en_HK
dc.identifier.issn1001-0742en_HK
dc.identifier.urihttp://hdl.handle.net/10722/73245-
dc.description.abstractThere were 6 target DNA fragments of the three parental strains existing in the cell of GEMs(genetically engineered microorganism strain) Fhhh measured in this research by PCR(polymerase chain reaction). The determination showed that GEMs Fhhh contained all the 6 target DNA fragments, mnp1, mnp2, lip1, lip2, FLO1 and 16S rDNA, and had the molecular genetic stability. Meanwhile the PCR production of each parental strain could only had its target DNA fragments and was different from each other. It may illustrate that the technique of the inter-kingdom protoplast fusion for the construction of GEMs Fhhh through the process of intercellular gene recombination could be used as a reliable bioengineering technique to create the specific functional stain for the pollution control.en_HK
dc.languageengen_HK
dc.publisherI O S Press. The Journal's web site is located at http://www.iospress.nl/html/10010742.phpen_HK
dc.relation.ispartofJournal of Environmental Sciencesen_HK
dc.rightsJournal of Environmental Sciences. Copyright © I O S Press.en_HK
dc.subjectDNA fragmenten_HK
dc.subjectGEMsen_HK
dc.subjectPCRen_HK
dc.subjectPollution controlen_HK
dc.subjectProtoplast fusionen_HK
dc.subjectTarget geneen_HK
dc.titleAnalysis of parental strain DNA fragments existing in GEMs-Fhhhen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1001-0742&volume=15&spage=590&epage=594&date=2003&atitle=Analysis+of+parental+strain+DNA+fragments+existing+in+GEMs-Fhhhen_HK
dc.identifier.emailGu, JD: jdgu@hkucc.hku.hken_HK
dc.identifier.authorityGu, JD=rp00701en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.pmid14562916-
dc.identifier.scopuseid_2-s2.0-0141795628en_HK
dc.identifier.hkuros86628en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0141795628&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume15en_HK
dc.identifier.issue5en_HK
dc.identifier.spage590en_HK
dc.identifier.epage594en_HK
dc.identifier.isiWOS:000185051200003-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridHao, CB=15762471600en_HK
dc.identifier.scopusauthoridYan, J=7403729064en_HK
dc.identifier.scopusauthoridQu, MM=11139459800en_HK
dc.identifier.scopusauthoridWang, D=8877064200en_HK
dc.identifier.scopusauthoridCheng, SP=18039405400en_HK
dc.identifier.scopusauthoridGu, JD=7403129601en_HK
dc.identifier.scopusauthoridQiu, WF=7202211475en_HK
dc.identifier.scopusauthoridWang, YY=7601509523en_HK

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