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Article: Long term effect of methylparathion contamination on soil microbial community diversity estimated by 16S rRNA gene cloning

TitleLong term effect of methylparathion contamination on soil microbial community diversity estimated by 16S rRNA gene cloning
Authors
KeywordsCulture-independent method
Dominant phylotype
Long-term methylparathion contamination
Microbial community
Novel bacterial division
Issue Date2006
PublisherSpringer New York LLC. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0963-9292
Citation
Ecotoxicology, 2006, v. 15 n. 6, p. 523-530 How to Cite?
AbstractThe long-term effects of methylparathion contamination on the diversity of soil microbial community was investigated by a culture-independent approach using small subunit ribosomal RNA (SSU rRNA) gene-based cloning. Microbial DNA extracted from both the control soil sample and methylparathion contaminated soil sample was subjected to PCR amplification with primers specific for bacterial 16S rRNA gene sequences. From the PCR amplification product, clone libraries were constructed for both samples. Phylotypes were defined by performing a restriction fragment length polymorphism analysis of 16S rRNA gene sequences with the enzymes RsaI and HhaI. A total of 603 phylotypes were identified among the 16S ribosomal DNA (rDNA) clones, the phylotype richness, frequency distribution (evenness) of the two clone libraries were compared by using a variety of diversity indices. Phylogenetic analysis of the sequences of the dominant phylotypes revealed that the bacterial communities changed noticeably. In the control soil, the dominant bacterial groups included a member of a novel bacterial division, the bacillus genus, and a member of α-proteobacteria, while in methylparathion contaminated soil, the dominant phylotypes were replaced by a member of the flexibactera-cytophaga-bacteroides division and two members of the γ-proteobacteria subdivision. This is the first report of the long-term effects of methylparathion (one of the major pesticides widely used in developing countries) on soil microbial community diversity and structure by a culture-independent method, and provides the evidences to assess the long-term environmental toxicological effects of methylparathion from the microbial community viewpoint. © Springer Science+Business Media, LLC 2006.
Persistent Identifierhttp://hdl.handle.net/10722/73205
ISSN
2015 Impact Factor: 2.329
2015 SCImago Journal Rankings: 1.108
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorZhang, Ren_HK
dc.contributor.authorJiang, Jen_HK
dc.contributor.authorGu, JDen_HK
dc.contributor.authorLi, Sen_HK
dc.date.accessioned2010-09-06T06:49:08Z-
dc.date.available2010-09-06T06:49:08Z-
dc.date.issued2006en_HK
dc.identifier.citationEcotoxicology, 2006, v. 15 n. 6, p. 523-530en_HK
dc.identifier.issn0963-9292en_HK
dc.identifier.urihttp://hdl.handle.net/10722/73205-
dc.description.abstractThe long-term effects of methylparathion contamination on the diversity of soil microbial community was investigated by a culture-independent approach using small subunit ribosomal RNA (SSU rRNA) gene-based cloning. Microbial DNA extracted from both the control soil sample and methylparathion contaminated soil sample was subjected to PCR amplification with primers specific for bacterial 16S rRNA gene sequences. From the PCR amplification product, clone libraries were constructed for both samples. Phylotypes were defined by performing a restriction fragment length polymorphism analysis of 16S rRNA gene sequences with the enzymes RsaI and HhaI. A total of 603 phylotypes were identified among the 16S ribosomal DNA (rDNA) clones, the phylotype richness, frequency distribution (evenness) of the two clone libraries were compared by using a variety of diversity indices. Phylogenetic analysis of the sequences of the dominant phylotypes revealed that the bacterial communities changed noticeably. In the control soil, the dominant bacterial groups included a member of a novel bacterial division, the bacillus genus, and a member of α-proteobacteria, while in methylparathion contaminated soil, the dominant phylotypes were replaced by a member of the flexibactera-cytophaga-bacteroides division and two members of the γ-proteobacteria subdivision. This is the first report of the long-term effects of methylparathion (one of the major pesticides widely used in developing countries) on soil microbial community diversity and structure by a culture-independent method, and provides the evidences to assess the long-term environmental toxicological effects of methylparathion from the microbial community viewpoint. © Springer Science+Business Media, LLC 2006.en_HK
dc.languageengen_HK
dc.publisherSpringer New York LLC. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0963-9292en_HK
dc.relation.ispartofEcotoxicologyen_HK
dc.subjectCulture-independent methoden_HK
dc.subjectDominant phylotypeen_HK
dc.subjectLong-term methylparathion contaminationen_HK
dc.subjectMicrobial communityen_HK
dc.subjectNovel bacterial divisionen_HK
dc.titleLong term effect of methylparathion contamination on soil microbial community diversity estimated by 16S rRNA gene cloningen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0963-9292&volume=15&spage=523&epage=530&date=2006&atitle=Long+term+effect+of+methylparathion+contamination+on+soil+microbial+community+diversity+estimated+by+16S+rRNA+gene+cloningen_HK
dc.identifier.emailGu, JD: jdgu@hkucc.hku.hken_HK
dc.identifier.authorityGu, JD=rp00701en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1007/s10646-006-0088-yen_HK
dc.identifier.pmid16816989-
dc.identifier.scopuseid_2-s2.0-33748985805en_HK
dc.identifier.hkuros134265en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-33748985805&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume15en_HK
dc.identifier.issue6en_HK
dc.identifier.spage523en_HK
dc.identifier.epage530en_HK
dc.identifier.isiWOS:000240719000004-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridZhang, R=8612262700en_HK
dc.identifier.scopusauthoridJiang, J=35240365600en_HK
dc.identifier.scopusauthoridGu, JD=7403129601en_HK
dc.identifier.scopusauthoridLi, S=24587265400en_HK

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