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Article: Identification and characterization of a "biomarker of toxicity" from the proteome of the paralytic shellfish toxin-producing dinoflagellate Alexandrium tamarense (Dinophyceae)

TitleIdentification and characterization of a "biomarker of toxicity" from the proteome of the paralytic shellfish toxin-producing dinoflagellate Alexandrium tamarense (Dinophyceae)
Authors
KeywordsAlexandrium tamarense
Biomarker of toxicity
Harmful algal bloom species
Paralytic shellfish poisoning toxin
Polyclonal antibodies
Issue Date2006
PublisherWiley - V C H Verlag GmbH & Co KGaA. The Journal's web site is located at http://www.wiley-vch.de/home/proteomics
Citation
Proteomics, 2006, v. 6 n. 2, p. 654-666 How to Cite?
AbstractThe objective of this study was to identify and characterize a "biomarker of toxicity" from the proteome of Alexandrium tamarense, a paralytic shellfish toxin (PST)-producing dinoflagellate. A combination of 2-DE and MS approaches was employed to identify proteins of interest in the vegetative cells of several strains of A. tamarense with different toxin compositions and from different geographical locations. The electrophoretic analysis of the total water-soluble proteins from these toxic strains by 2-DE showed that several abundant proteins, namely AT-T1, AT-T2 and AT-T3, differing slightly in apparent M r and pIs, were consistently present in all toxic strains of A. tamarense. Further analysis by MALDI-TOF MS and N-terminal amino acid sequencing revealed that they are isoforms of the same protein. Even more intriguing is that these proteins in A. tamarense have similar amino acid sequences and are closely related to a "biomarker of toxicity" previously reported in A. minutum. Unambiguous and highly species-specific identification was later achieved by comparing the PMFs of proteins in these two species. An initial attempt to characterize these proteins by generation of murine polyclonal antibodies against the ATT1 protein was successful. Western blot analysis using the murine AT-T1-polycolonal antibodies identified all the toxic strains of A. tamarense and A. minutum, but not the nontoxic strain of A. tamarense. These results indicate that these protein characteristics for toxic strains are species-specific and that they are stable properties of the tested algae which are clearly distinguishable irrespective of geographical location and toxin composition. To our knowledge, this is the first study to demonstrate the use of polyclonal antibodies against marker proteins purified from 2-DE gels to distinguish different strains and species of the PST-producing dinoflagellate Alexandrium. It provides the basis for the production of monoclonal antibody probes against the "biomarkers of toxicity" for those dinoflagellates whose genome is incompletely characterized. Potentially, immunoassays could be developed to detect the presence of toxic algae in routine monitoring programs as well as to predict bloom development and movement. © 2006 Wiley-VCH Verlag GmbH & Co. KGaA.
Persistent Identifierhttp://hdl.handle.net/10722/73193
ISSN
2015 Impact Factor: 4.079
2015 SCImago Journal Rankings: 1.476
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorChan, LLen_HK
dc.contributor.authorSit, WHen_HK
dc.contributor.authorLam, PKSen_HK
dc.contributor.authorHsieh, DPHen_HK
dc.contributor.authorHodgkiss, IJen_HK
dc.contributor.authorWan, JMFen_HK
dc.contributor.authorHo, AYTen_HK
dc.contributor.authorChoi, NMCen_HK
dc.contributor.authorWang, DZen_HK
dc.contributor.authorDudgeon, Den_HK
dc.date.accessioned2010-09-06T06:49:01Z-
dc.date.available2010-09-06T06:49:01Z-
dc.date.issued2006en_HK
dc.identifier.citationProteomics, 2006, v. 6 n. 2, p. 654-666en_HK
dc.identifier.issn1615-9853en_HK
dc.identifier.urihttp://hdl.handle.net/10722/73193-
dc.description.abstractThe objective of this study was to identify and characterize a "biomarker of toxicity" from the proteome of Alexandrium tamarense, a paralytic shellfish toxin (PST)-producing dinoflagellate. A combination of 2-DE and MS approaches was employed to identify proteins of interest in the vegetative cells of several strains of A. tamarense with different toxin compositions and from different geographical locations. The electrophoretic analysis of the total water-soluble proteins from these toxic strains by 2-DE showed that several abundant proteins, namely AT-T1, AT-T2 and AT-T3, differing slightly in apparent M r and pIs, were consistently present in all toxic strains of A. tamarense. Further analysis by MALDI-TOF MS and N-terminal amino acid sequencing revealed that they are isoforms of the same protein. Even more intriguing is that these proteins in A. tamarense have similar amino acid sequences and are closely related to a "biomarker of toxicity" previously reported in A. minutum. Unambiguous and highly species-specific identification was later achieved by comparing the PMFs of proteins in these two species. An initial attempt to characterize these proteins by generation of murine polyclonal antibodies against the ATT1 protein was successful. Western blot analysis using the murine AT-T1-polycolonal antibodies identified all the toxic strains of A. tamarense and A. minutum, but not the nontoxic strain of A. tamarense. These results indicate that these protein characteristics for toxic strains are species-specific and that they are stable properties of the tested algae which are clearly distinguishable irrespective of geographical location and toxin composition. To our knowledge, this is the first study to demonstrate the use of polyclonal antibodies against marker proteins purified from 2-DE gels to distinguish different strains and species of the PST-producing dinoflagellate Alexandrium. It provides the basis for the production of monoclonal antibody probes against the "biomarkers of toxicity" for those dinoflagellates whose genome is incompletely characterized. Potentially, immunoassays could be developed to detect the presence of toxic algae in routine monitoring programs as well as to predict bloom development and movement. © 2006 Wiley-VCH Verlag GmbH & Co. KGaA.en_HK
dc.languageengen_HK
dc.publisherWiley - V C H Verlag GmbH & Co KGaA. The Journal's web site is located at http://www.wiley-vch.de/home/proteomicsen_HK
dc.relation.ispartofProteomicsen_HK
dc.subjectAlexandrium tamarenseen_HK
dc.subjectBiomarker of toxicityen_HK
dc.subjectHarmful algal bloom speciesen_HK
dc.subjectParalytic shellfish poisoning toxinen_HK
dc.subjectPolyclonal antibodiesen_HK
dc.titleIdentification and characterization of a "biomarker of toxicity" from the proteome of the paralytic shellfish toxin-producing dinoflagellate Alexandrium tamarense (Dinophyceae)en_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1615-9853&volume=6&spage=654&epage=666&date=2006&atitle=Identification+and+characterization+of+a+%27biomarker+of+toxicity%27+from+the+proteome+of+the+paralytic+shellfish+toxin-producing+dinoflagellate+Alexandrium+tamarense+(Dinophyceae)en_HK
dc.identifier.emailWan, JMF: jmfwan@hku.hken_HK
dc.identifier.emailDudgeon, D: ddudgeon@hku.hken_HK
dc.identifier.authorityWan, JMF=rp00798en_HK
dc.identifier.authorityDudgeon, D=rp00691en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1002/pmic.200401350en_HK
dc.identifier.pmid16342137-
dc.identifier.scopuseid_2-s2.0-32144460520en_HK
dc.identifier.hkuros123607en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-32144460520&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume6en_HK
dc.identifier.issue2en_HK
dc.identifier.spage654en_HK
dc.identifier.epage666en_HK
dc.identifier.isiWOS:000235207700028-
dc.publisher.placeGermanyen_HK
dc.identifier.scopusauthoridChan, LL=35757489200en_HK
dc.identifier.scopusauthoridSit, WH=8528923000en_HK
dc.identifier.scopusauthoridLam, PKS=7202365776en_HK
dc.identifier.scopusauthoridHsieh, DPH=7103265321en_HK
dc.identifier.scopusauthoridHodgkiss, IJ=7006614647en_HK
dc.identifier.scopusauthoridWan, JMF=8930305000en_HK
dc.identifier.scopusauthoridHo, AYT=23667369600en_HK
dc.identifier.scopusauthoridChoi, NMC=12239088700en_HK
dc.identifier.scopusauthoridWang, DZ=7407075632en_HK
dc.identifier.scopusauthoridDudgeon, D=7006559840en_HK

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