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Article: Hexavalent chromium induced stress and metabolic responses in hybrid willows

TitleHexavalent chromium induced stress and metabolic responses in hybrid willows
Authors
KeywordsAccumulation
Enzyme
Hexavalent chromium (Cr6+)
Oxidative stress
Phytoremediation
Translocation
Willows
Issue Date2007
PublisherSpringer New York LLC. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0963-9292
Citation
Ecotoxicology, 2007, v. 16 n. 3, p. 299-309 How to Cite?
AbstractMetabolic responses to hexavalent chromium (Cr6+) stress and the uptake and translocation of Cr6+ were investigated using pre-rooted hybrid willows (Salix matsudana Koidz x Salix alba L.) exposed to hydroponic solution spiked with K2CrO4 at 24.0 ± 1°C for 192 h. Various physiological parameters of the plants were monitored to determine toxicity from Cr6+ exposure. At Cr6+ treatments of ≤2.1 mg Cr/l, the transpiration rate of plants was > 50% higher than that of the non-treated control plants. As Cr concentrations were increased further, a slight increase in the transpiration rate was also observed compared with the controls. Negligible difference in the chlorophyll contents in leaves between the treated and the non-treated control plants was measured, except for willows exposed to 1.05 mg Cr/l. The response of soluble proteins in leaves of willows to Cr treatments was remarkable. Cr-induced toxicity appeared in all treatments resulting in reduced activities of catalase (CAT) and peroxidase (POD) compared to the controls. Superoxide dismutases (SOD) activity in the leaf cells showed a positive increase after Cr exposure. Of all selected parameters, soluble proteins in leaves were the most sensitive to Cr6+ doses, showing a significant linear correlation negatively (R 2 = 0.931). Uptake of Cr6+ by willows grown in flasks was found to increase linearly with the added Cr6+ (a zero order kinetics), as indicated by the high R 2 (0.9322). Recovery of Cr in different parts of plant materials varied significantly with roots being the dominant site of Cr accumulation. Although the translocation to shoots was detected, the amount of Cr translocated to shoots was considerably small. The capacity of willows to assimilate Cr6+ was also evaluated using detached leaves and roots in sealed glass vessels in vivo. Uptake of Cr by roots was mediated possibly through an active transport mechanism, whereas the cuticle of leaves was the major obstacle to uptake Cr from the hydroponic solution. In addition, both cysteine and ascorbic acid showed a remarkable potential to reduce Cr 6+ at a neutral pH. Results indicated that the added Cr did not cause deleterious effects on plant physiological functions over a 192-h period of exposure. Significant removal of Cr from the hydroponic solution was observed in the presence of hybrid willows. The data also suggest that phytoremediation of Cr6+ is possible and ecologically safe due to the minor translocation of Cr to aerial tissues. © 2007 Springer Science+Business Media, LLC.
Persistent Identifierhttp://hdl.handle.net/10722/73177
ISSN
2015 Impact Factor: 2.329
2015 SCImago Journal Rankings: 1.108
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorYu, XZen_HK
dc.contributor.authorGu, JDen_HK
dc.contributor.authorHuang, SZen_HK
dc.date.accessioned2010-09-06T06:48:53Z-
dc.date.available2010-09-06T06:48:53Z-
dc.date.issued2007en_HK
dc.identifier.citationEcotoxicology, 2007, v. 16 n. 3, p. 299-309en_HK
dc.identifier.issn0963-9292en_HK
dc.identifier.urihttp://hdl.handle.net/10722/73177-
dc.description.abstractMetabolic responses to hexavalent chromium (Cr6+) stress and the uptake and translocation of Cr6+ were investigated using pre-rooted hybrid willows (Salix matsudana Koidz x Salix alba L.) exposed to hydroponic solution spiked with K2CrO4 at 24.0 ± 1°C for 192 h. Various physiological parameters of the plants were monitored to determine toxicity from Cr6+ exposure. At Cr6+ treatments of ≤2.1 mg Cr/l, the transpiration rate of plants was > 50% higher than that of the non-treated control plants. As Cr concentrations were increased further, a slight increase in the transpiration rate was also observed compared with the controls. Negligible difference in the chlorophyll contents in leaves between the treated and the non-treated control plants was measured, except for willows exposed to 1.05 mg Cr/l. The response of soluble proteins in leaves of willows to Cr treatments was remarkable. Cr-induced toxicity appeared in all treatments resulting in reduced activities of catalase (CAT) and peroxidase (POD) compared to the controls. Superoxide dismutases (SOD) activity in the leaf cells showed a positive increase after Cr exposure. Of all selected parameters, soluble proteins in leaves were the most sensitive to Cr6+ doses, showing a significant linear correlation negatively (R 2 = 0.931). Uptake of Cr6+ by willows grown in flasks was found to increase linearly with the added Cr6+ (a zero order kinetics), as indicated by the high R 2 (0.9322). Recovery of Cr in different parts of plant materials varied significantly with roots being the dominant site of Cr accumulation. Although the translocation to shoots was detected, the amount of Cr translocated to shoots was considerably small. The capacity of willows to assimilate Cr6+ was also evaluated using detached leaves and roots in sealed glass vessels in vivo. Uptake of Cr by roots was mediated possibly through an active transport mechanism, whereas the cuticle of leaves was the major obstacle to uptake Cr from the hydroponic solution. In addition, both cysteine and ascorbic acid showed a remarkable potential to reduce Cr 6+ at a neutral pH. Results indicated that the added Cr did not cause deleterious effects on plant physiological functions over a 192-h period of exposure. Significant removal of Cr from the hydroponic solution was observed in the presence of hybrid willows. The data also suggest that phytoremediation of Cr6+ is possible and ecologically safe due to the minor translocation of Cr to aerial tissues. © 2007 Springer Science+Business Media, LLC.en_HK
dc.languageengen_HK
dc.publisherSpringer New York LLC. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0963-9292en_HK
dc.relation.ispartofEcotoxicologyen_HK
dc.subjectAccumulationen_HK
dc.subjectEnzymeen_HK
dc.subjectHexavalent chromium (Cr6+)en_HK
dc.subjectOxidative stressen_HK
dc.subjectPhytoremediationen_HK
dc.subjectTranslocationen_HK
dc.subjectWillowsen_HK
dc.titleHexavalent chromium induced stress and metabolic responses in hybrid willowsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0963-9292&volume=16&spage=299&epage=309&date=2007&atitle=Hexavalent+chromium+induced+stress+and+metabolic+responses+in+hybrid+willowsen_HK
dc.identifier.emailGu, JD: jdgu@hkucc.hku.hken_HK
dc.identifier.authorityGu, JD=rp00701en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1007/s10646-006-0129-6en_HK
dc.identifier.pmid17253159-
dc.identifier.scopuseid_2-s2.0-33947327305en_HK
dc.identifier.hkuros134266en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-33947327305&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume16en_HK
dc.identifier.issue3en_HK
dc.identifier.spage299en_HK
dc.identifier.epage309en_HK
dc.identifier.isiWOS:000245471300002-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridYu, XZ=24449490500en_HK
dc.identifier.scopusauthoridGu, JD=7403129601en_HK
dc.identifier.scopusauthoridHuang, SZ=16047520200en_HK
dc.identifier.citeulike1238790-

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