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Article: Simultaneous biodegradation of methyl parathion and carbofuran by a genetically engineered microorganism constructed by mini-Tn5 transposon

TitleSimultaneous biodegradation of methyl parathion and carbofuran by a genetically engineered microorganism constructed by mini-Tn5 transposon
Authors
KeywordsBiodegradation
Carbofuran
Genetically engineered microorganism
Methyl parathion
Mini-transposon
Issue Date2007
PublisherSpringer Verlag Dordrecht. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0923-9820
Citation
Biodegradation, 2007, v. 18 n. 4, p. 403-412 How to Cite?
AbstractA genetically engineered microorganism (GEM) capable of simultaneous degrading methyl parathion (MP) and carbofuran was successfully constructed by random insertion of a methyl parathion hydrolase gene (mpd) into the chromosome of a carbofuran degrading Sphingomonas sp. CDS-1 with the mini-transposon system. The GEM constructed was relatively stable and cell viability and original degrading characteristic was not affected compared with the original recipient CDS-1. The effects of temperature, initial pH value, inoculum size and alternative carbon source on the biodegradation of MP and carbofuran were investigated. GEM cells could degrade MP and carbofuran efficiently in a relatively broad range of temperatures from 20 to 30°C, initial pH values from 6.0 to 9.0, and with all initial inoculation cell densities (10 5-10 7 CFU ml -1), even if alternative glucose existed. The optimal temperature and initial pH value for GEM cells to simultaneously degrade MP and carbofuran was at 30°C and at pH 7.0. The removal of MP and carbofuran by GEM cells in sterile and non-sterile soil were also studied. In both soil samples, 50 mg kg -1 MP and 25 mg kg -1 carbofuran could be degraded to an undetectable level within 25 days even if there were indigenous microbial competition and carbon sources effect. In sterile soil, the biodegradation rates of MP and carbofuran were faster, and the decline of the inoculated GEM cells was slower compared with that in non-sterile soil. The GEM constructed in this study was potential useful for pesticides bioremediation in natural environment. © 2006 Springer Science+Business Media B.V.
Persistent Identifierhttp://hdl.handle.net/10722/73173
ISSN
2015 Impact Factor: 2.208
2015 SCImago Journal Rankings: 0.907
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorJiang, Jen_HK
dc.contributor.authorZhang, Ren_HK
dc.contributor.authorLi, Ren_HK
dc.contributor.authorGu, JDen_HK
dc.contributor.authorLi, Sen_HK
dc.date.accessioned2010-09-06T06:48:50Z-
dc.date.available2010-09-06T06:48:50Z-
dc.date.issued2007en_HK
dc.identifier.citationBiodegradation, 2007, v. 18 n. 4, p. 403-412en_HK
dc.identifier.issn0923-9820en_HK
dc.identifier.urihttp://hdl.handle.net/10722/73173-
dc.description.abstractA genetically engineered microorganism (GEM) capable of simultaneous degrading methyl parathion (MP) and carbofuran was successfully constructed by random insertion of a methyl parathion hydrolase gene (mpd) into the chromosome of a carbofuran degrading Sphingomonas sp. CDS-1 with the mini-transposon system. The GEM constructed was relatively stable and cell viability and original degrading characteristic was not affected compared with the original recipient CDS-1. The effects of temperature, initial pH value, inoculum size and alternative carbon source on the biodegradation of MP and carbofuran were investigated. GEM cells could degrade MP and carbofuran efficiently in a relatively broad range of temperatures from 20 to 30°C, initial pH values from 6.0 to 9.0, and with all initial inoculation cell densities (10 5-10 7 CFU ml -1), even if alternative glucose existed. The optimal temperature and initial pH value for GEM cells to simultaneously degrade MP and carbofuran was at 30°C and at pH 7.0. The removal of MP and carbofuran by GEM cells in sterile and non-sterile soil were also studied. In both soil samples, 50 mg kg -1 MP and 25 mg kg -1 carbofuran could be degraded to an undetectable level within 25 days even if there were indigenous microbial competition and carbon sources effect. In sterile soil, the biodegradation rates of MP and carbofuran were faster, and the decline of the inoculated GEM cells was slower compared with that in non-sterile soil. The GEM constructed in this study was potential useful for pesticides bioremediation in natural environment. © 2006 Springer Science+Business Media B.V.en_HK
dc.languageengen_HK
dc.publisherSpringer Verlag Dordrecht. The Journal's web site is located at http://springerlink.metapress.com/openurl.asp?genre=journal&issn=0923-9820en_HK
dc.relation.ispartofBiodegradationen_HK
dc.subjectBiodegradationen_HK
dc.subjectCarbofuranen_HK
dc.subjectGenetically engineered microorganismen_HK
dc.subjectMethyl parathionen_HK
dc.subjectMini-transposonen_HK
dc.titleSimultaneous biodegradation of methyl parathion and carbofuran by a genetically engineered microorganism constructed by mini-Tn5 transposonen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0923-9820&volume=18&spage=403&epage=412&date=2007&atitle=Simultaneous+biodegradation+of+methyl+parathion+and+carbofuran+by+a+genetically+engineered+microorganism+constructed+by+mini-Tn5+transposonen_HK
dc.identifier.emailGu, JD: jdgu@hkucc.hku.hken_HK
dc.identifier.authorityGu, JD=rp00701en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1007/s10532-006-9075-5en_HK
dc.identifier.pmid17091349-
dc.identifier.scopuseid_2-s2.0-34447107621en_HK
dc.identifier.hkuros134261en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-34447107621&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume18en_HK
dc.identifier.issue4en_HK
dc.identifier.spage403en_HK
dc.identifier.epage412en_HK
dc.identifier.isiWOS:000247929200002-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridJiang, J=35240365600en_HK
dc.identifier.scopusauthoridZhang, R=8612262700en_HK
dc.identifier.scopusauthoridLi, R=35223325100en_HK
dc.identifier.scopusauthoridGu, JD=7403129601en_HK
dc.identifier.scopusauthoridLi, S=24587265400en_HK
dc.identifier.citeulike1603059-

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