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Article: Comparative proteomic analysis of the esophageal squamous carcinoma cell line EC109 and its multi-drug resistant subline EC109/CDDP

TitleComparative proteomic analysis of the esophageal squamous carcinoma cell line EC109 and its multi-drug resistant subline EC109/CDDP
Authors
KeywordsEsophageal squamous cell carcinoma
Multidrug resistance
Proteomics
Issue Date2010
PublisherSpandidos Publications. The Journal's web site is located at http://www.spandidos-publications.com/ijo/
Citation
International Journal Of Oncology, 2010, v. 36 n. 1, p. 265-274 How to Cite?
AbstractTo gain insights into the mechanisms of drug resistance in esophageal squamous cell carcinoma (ESCC), we employed proteomic techniques to study the global protein change of the multi-drug resistant ESCC cell line EC109/CDDP, which was established in our previous work, in comparison with its parental drug sensitive cell line EC109. By two-dimensional electrophoresis and mass spectrometry, we successfully identified 44 proteins with altered expression levels. These proteins are involved in endoplasmic reticulum stress response, metabolic process, DNA replication and repair, nucleotide binding, calcium binding, and cytoskeletal proteins. Among them, the differential expression levels of thioredoxin domain-containing protein 4 precursor and cystathionine γ-lyase were further validated by Western blot and RT-PCR. Our present results lay foundation for future in-depth work on molecular mechanism of ESCC drug resistance, and aid in the identification and use of novel markers in clinical practice.
Persistent Identifierhttp://hdl.handle.net/10722/72041
ISSN
2015 Impact Factor: 3.018
2015 SCImago Journal Rankings: 1.270
ISI Accession Number ID
Funding AgencyGrant Number
Guangdong Natural Science Foundation7001531
Funding Information:

The study was supported by Guangdong Natural Science Foundation (Grants No. 7001531).

References

 

DC FieldValueLanguage
dc.contributor.authorWen, Jen_HK
dc.contributor.authorZheng, Ben_HK
dc.contributor.authorHu, Yen_HK
dc.contributor.authorZhang, Xen_HK
dc.contributor.authorYang, Hen_HK
dc.contributor.authorLi, Yen_HK
dc.contributor.authorZhang, CYen_HK
dc.contributor.authorLuo, KJen_HK
dc.contributor.authorZang, Xen_HK
dc.contributor.authorLi, YFen_HK
dc.contributor.authorGuan, XYen_HK
dc.contributor.authorFu, JHen_HK
dc.date.accessioned2010-09-06T06:37:46Z-
dc.date.available2010-09-06T06:37:46Z-
dc.date.issued2010en_HK
dc.identifier.citationInternational Journal Of Oncology, 2010, v. 36 n. 1, p. 265-274en_HK
dc.identifier.issn1019-6439en_HK
dc.identifier.urihttp://hdl.handle.net/10722/72041-
dc.description.abstractTo gain insights into the mechanisms of drug resistance in esophageal squamous cell carcinoma (ESCC), we employed proteomic techniques to study the global protein change of the multi-drug resistant ESCC cell line EC109/CDDP, which was established in our previous work, in comparison with its parental drug sensitive cell line EC109. By two-dimensional electrophoresis and mass spectrometry, we successfully identified 44 proteins with altered expression levels. These proteins are involved in endoplasmic reticulum stress response, metabolic process, DNA replication and repair, nucleotide binding, calcium binding, and cytoskeletal proteins. Among them, the differential expression levels of thioredoxin domain-containing protein 4 precursor and cystathionine γ-lyase were further validated by Western blot and RT-PCR. Our present results lay foundation for future in-depth work on molecular mechanism of ESCC drug resistance, and aid in the identification and use of novel markers in clinical practice.en_HK
dc.languageengen_HK
dc.publisherSpandidos Publications. The Journal's web site is located at http://www.spandidos-publications.com/ijo/en_HK
dc.relation.ispartofInternational Journal of Oncologyen_HK
dc.subjectEsophageal squamous cell carcinomaen_HK
dc.subjectMultidrug resistanceen_HK
dc.subjectProteomicsen_HK
dc.subject.meshAntineoplastic Agents - pharmacology-
dc.subject.meshCarcinoma, Squamous Cell - metabolism-
dc.subject.meshCisplatin - pharmacology-
dc.subject.meshDrug Resistance, Neoplasm-
dc.subject.meshEsophageal Neoplasms - metabolism-
dc.titleComparative proteomic analysis of the esophageal squamous carcinoma cell line EC109 and its multi-drug resistant subline EC109/CDDPen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1019-6439&volume=36&issue=1&spage=265&epage=274&date=2010&atitle=Comparative+proteomic+analysis+of+the+esophageal+squamous+carcinoma+cell+line+EC109+and+its+multi-drug+resistant+subline+EC109/CDDPen_HK
dc.identifier.emailGuan, XY:xyguan@hkucc.hku.hken_HK
dc.identifier.authorityGuan, XY=rp00454en_HK
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.3892/ijo_00000497en_HK
dc.identifier.pmid19956855en_HK
dc.identifier.scopuseid_2-s2.0-74549121530en_HK
dc.identifier.hkuros169982en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-74549121530&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume36en_HK
dc.identifier.issue1en_HK
dc.identifier.spage265en_HK
dc.identifier.epage274en_HK
dc.identifier.isiWOS:000272877100028-
dc.publisher.placeGreeceen_HK
dc.identifier.scopusauthoridWen, J=7402701826en_HK
dc.identifier.scopusauthoridZheng, B=35319446400en_HK
dc.identifier.scopusauthoridHu, Y=7407115980en_HK
dc.identifier.scopusauthoridZhang, X=35108652900en_HK
dc.identifier.scopusauthoridYang, H=7406561423en_HK
dc.identifier.scopusauthoridLi, Y=36078824800en_HK
dc.identifier.scopusauthoridZhang, CY=14033447100en_HK
dc.identifier.scopusauthoridLuo, KJ=25028129100en_HK
dc.identifier.scopusauthoridZang, X=35742430200en_HK
dc.identifier.scopusauthoridLi, YF=35215536300en_HK
dc.identifier.scopusauthoridGuan, XY=7201463221en_HK
dc.identifier.scopusauthoridFu, JH=8228815900en_HK

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