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Article: Heterogeneous expression and association of β-catenin, p16 and c-myc in multistage colorectal tumorigenesis and progression detected by tissue microarray

TitleHeterogeneous expression and association of β-catenin, p16 and c-myc in multistage colorectal tumorigenesis and progression detected by tissue microarray
Authors
KeywordsColorectal carcinoma
Fluorescence in situ hybridization
Heterogeneity
Immunohistochemistry
Issue Date2003
PublisherJohn Wiley & Sons, Inc.. The Journal's web site is located at http://www3.interscience.wiley.com/journal/29331/home
Citation
International Journal Of Cancer, 2003, v. 107 n. 6, p. 896-902 How to Cite?
AbstractMost colorectal carcinomas (CRCs) arise from adenomas through an archetypal pathogenic pathway, the adenomacarcinoma-metastasis sequence. Aberrant expression of β-catenin, p 16, E-cadherin and c-myc appears to have played important roles in the development and/or progression of CRC, but their precise distribution pattern and associations in different pathologic loci along CRC's pathogenic pathway have not been thoroughly examined. In this study, a tissue microarray (TMA) containing 85 advanced CRCs in different Dukes stages was constructed. In each of 85 cases, tissue specimens from normal mucosa and primary carcinomas in different layers of the bowel wall were included in the TMA. Tissue specimens from matched adenoma, lymph node metastases and distant metastases were obtained from 22, 21 and 21 cases, respectively. Expression patterns of β-catenin, p16, E-cadherin and c-myc were evaluated by immunohistochemistry. The results revealed that nuclear expression of β-catenin, p16 and c-myc was quantitatively increased from normal mucosa to premalignant adenoma, primary carcinoma and lymph node metastatic carcinoma; the frequency of nuclear overexpression of β-catenin and p 16 in lymph node metastases was significantly higher than that in distant metastases (p < 0.05). These results suggest an association between nuclear overexpression of β-catenin and/or p 16 and CRC lymph node metastasis but not distant metastasis. The results also showed that correlative high nuclear expression of β-catenin and c-myc was observed in primary carcinomas involving the serosa and lymph node metastases (p < 0.05) but not in other pathologic regions of CRCs, suggesting that the tumor microenvironment in different pathologic loci of colorectal tumorigenesis and progression may influence c-myc responsiveness to β-catenin/Tcf activation. © 2003 Wiley-Liss, Inc.
Persistent Identifierhttp://hdl.handle.net/10722/72039
ISSN
2015 Impact Factor: 5.531
2015 SCImago Journal Rankings: 2.657
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorXie, Den_HK
dc.contributor.authorSham, JSTen_HK
dc.contributor.authorZeng, WFen_HK
dc.contributor.authorLin, HLen_HK
dc.contributor.authorChe, LHen_HK
dc.contributor.authorWu, HXen_HK
dc.contributor.authorWen, JMen_HK
dc.contributor.authorFang, Yen_HK
dc.contributor.authorHu, Len_HK
dc.contributor.authorGuan, XYen_HK
dc.date.accessioned2010-09-06T06:37:45Z-
dc.date.available2010-09-06T06:37:45Z-
dc.date.issued2003en_HK
dc.identifier.citationInternational Journal Of Cancer, 2003, v. 107 n. 6, p. 896-902en_HK
dc.identifier.issn0020-7136en_HK
dc.identifier.urihttp://hdl.handle.net/10722/72039-
dc.description.abstractMost colorectal carcinomas (CRCs) arise from adenomas through an archetypal pathogenic pathway, the adenomacarcinoma-metastasis sequence. Aberrant expression of β-catenin, p 16, E-cadherin and c-myc appears to have played important roles in the development and/or progression of CRC, but their precise distribution pattern and associations in different pathologic loci along CRC's pathogenic pathway have not been thoroughly examined. In this study, a tissue microarray (TMA) containing 85 advanced CRCs in different Dukes stages was constructed. In each of 85 cases, tissue specimens from normal mucosa and primary carcinomas in different layers of the bowel wall were included in the TMA. Tissue specimens from matched adenoma, lymph node metastases and distant metastases were obtained from 22, 21 and 21 cases, respectively. Expression patterns of β-catenin, p16, E-cadherin and c-myc were evaluated by immunohistochemistry. The results revealed that nuclear expression of β-catenin, p16 and c-myc was quantitatively increased from normal mucosa to premalignant adenoma, primary carcinoma and lymph node metastatic carcinoma; the frequency of nuclear overexpression of β-catenin and p 16 in lymph node metastases was significantly higher than that in distant metastases (p < 0.05). These results suggest an association between nuclear overexpression of β-catenin and/or p 16 and CRC lymph node metastasis but not distant metastasis. The results also showed that correlative high nuclear expression of β-catenin and c-myc was observed in primary carcinomas involving the serosa and lymph node metastases (p < 0.05) but not in other pathologic regions of CRCs, suggesting that the tumor microenvironment in different pathologic loci of colorectal tumorigenesis and progression may influence c-myc responsiveness to β-catenin/Tcf activation. © 2003 Wiley-Liss, Inc.en_HK
dc.languageengen_HK
dc.publisherJohn Wiley & Sons, Inc.. The Journal's web site is located at http://www3.interscience.wiley.com/journal/29331/homeen_HK
dc.relation.ispartofInternational Journal of Canceren_HK
dc.rightsInternational Journal of Cancer. Copyright © John Wiley & Sons, Inc..en_HK
dc.subjectColorectal carcinomaen_HK
dc.subjectFluorescence in situ hybridizationen_HK
dc.subjectHeterogeneityen_HK
dc.subjectImmunohistochemistryen_HK
dc.subject.meshColorectal Neoplasms - genetics - pathology-
dc.subject.meshCyclin-Dependent Kinase Inhibitor p16 - genetics-
dc.subject.meshCytoskeletal Proteins - genetics-
dc.subject.meshGene Expression Regulation, Neoplastic-
dc.subject.meshOligonucleotide Array Sequence Analysis - methods-
dc.titleHeterogeneous expression and association of β-catenin, p16 and c-myc in multistage colorectal tumorigenesis and progression detected by tissue microarrayen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0020-7136&volume=107&issue=6&spage=896&epage=902&date=2003&atitle=Heterogeneous+expression+and+association+of+β-catenin,+p16+and+c-myc+in+multistage+colorectal+tumorigenesis+and+progression+detected+by+tissue+microarrayen_HK
dc.identifier.emailGuan, XY:xyguan@hkucc.hku.hken_HK
dc.identifier.authorityGuan, XY=rp00454en_HK
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1002/ijc.11514en_HK
dc.identifier.pmid14601048-
dc.identifier.scopuseid_2-s2.0-10744228145en_HK
dc.identifier.hkuros96081en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-10744228145&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume107en_HK
dc.identifier.issue6en_HK
dc.identifier.spage896en_HK
dc.identifier.epage902en_HK
dc.identifier.isiWOS:000186619300005-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridXie, D=35070710200en_HK
dc.identifier.scopusauthoridSham, JST=7101655565en_HK
dc.identifier.scopusauthoridZeng, WF=8338623800en_HK
dc.identifier.scopusauthoridLin, HL=8950219500en_HK
dc.identifier.scopusauthoridChe, LH=7003959690en_HK
dc.identifier.scopusauthoridWu, HX=36189521500en_HK
dc.identifier.scopusauthoridWen, JM=7402701931en_HK
dc.identifier.scopusauthoridFang, Y=7403457405en_HK
dc.identifier.scopusauthoridHu, L=34770075600en_HK
dc.identifier.scopusauthoridGuan, XY=7201463221en_HK

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