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Article: High frequency of promoter hypermethylation of the death-associated protein-kinase gene in nasopharyngeal carcinoma and its detection in the peripheral blood of patients

TitleHigh frequency of promoter hypermethylation of the death-associated protein-kinase gene in nasopharyngeal carcinoma and its detection in the peripheral blood of patients
Authors
Issue Date2002
PublisherAmerican Association for Cancer Research.
Citation
Clinical Cancer Research, 2002, v. 8 n. 2, p. 433-437 How to Cite?
AbstractPurpose: Death-associated protein (DAP)-kinase gene is frequently inactivated by promoter hypermethylation in cancer. The aim of this study was to evaluate the promoter methylation status of the DAP-kinase gene in nasopharyngeal carcinoma (NPC). Experimental design: The methylation status was evaluated by methylation-specific PCR (MSP). Thirty-two NPC biopsy specimens, plasma and buffy coat of 12 patients, 5 NPC cell lines, 3 normal nasopharyngeal biopsy tissues, and 2 normal nasopharyngeal epithelial primary cultures were included in this study. Results: There was no promoter hypermethylation in all 3 normal nasopharyngeal tissues and 2 normal nasopharyngeal primary cultures. Hypermethylation was found in 24 (75%) NPC primary tumor biopsies and 4 (80%) NPC cell lines. Of the 24 patients with hypermethylation of DAPkinase promoter in the primary tumors, 12 patients had their plasma and buffy coat DNA available for MSP study. Hypermethylated DAP-kinase promoter was detectable in 5 patients in the plasma but not in the buffy coat, 2 patients in the buffy coat but not in the plasma, and 1 patient in both plasma and buffy coat. Four patients had no detectable hypermethylated DAP-kinase promoter in both plasma and buffy coat. Hypermethylation of DAP-kinase promoter was found in both early- and late-stage NPC. Conclusions: Our results show that hypermethylation of the DAP-kinase promoter is a common early event in NPC. The high frequency of identification of hypermethylated DAP-kinase promoter in plasma and buffy coat of NPC patients illustrates its potential clinical application as tumor marker for the diagnosis and monitoring of treatment result.
Persistent Identifierhttp://hdl.handle.net/10722/72034
ISSN
2015 Impact Factor: 8.738
2015 SCImago Journal Rankings: 5.314
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorThian, SWen_HK
dc.contributor.authorHsiao, WCen_HK
dc.contributor.authorKwong, CTen_HK
dc.contributor.authorWilliam, IWen_HK
dc.contributor.authorKwong, DLWen_HK
dc.contributor.authorSham, JSTen_HK
dc.contributor.authorYuen, APWen_HK
dc.contributor.authorYok, LKen_HK
dc.date.accessioned2010-09-06T06:37:42Z-
dc.date.available2010-09-06T06:37:42Z-
dc.date.issued2002en_HK
dc.identifier.citationClinical Cancer Research, 2002, v. 8 n. 2, p. 433-437en_HK
dc.identifier.issn1078-0432en_HK
dc.identifier.urihttp://hdl.handle.net/10722/72034-
dc.description.abstractPurpose: Death-associated protein (DAP)-kinase gene is frequently inactivated by promoter hypermethylation in cancer. The aim of this study was to evaluate the promoter methylation status of the DAP-kinase gene in nasopharyngeal carcinoma (NPC). Experimental design: The methylation status was evaluated by methylation-specific PCR (MSP). Thirty-two NPC biopsy specimens, plasma and buffy coat of 12 patients, 5 NPC cell lines, 3 normal nasopharyngeal biopsy tissues, and 2 normal nasopharyngeal epithelial primary cultures were included in this study. Results: There was no promoter hypermethylation in all 3 normal nasopharyngeal tissues and 2 normal nasopharyngeal primary cultures. Hypermethylation was found in 24 (75%) NPC primary tumor biopsies and 4 (80%) NPC cell lines. Of the 24 patients with hypermethylation of DAPkinase promoter in the primary tumors, 12 patients had their plasma and buffy coat DNA available for MSP study. Hypermethylated DAP-kinase promoter was detectable in 5 patients in the plasma but not in the buffy coat, 2 patients in the buffy coat but not in the plasma, and 1 patient in both plasma and buffy coat. Four patients had no detectable hypermethylated DAP-kinase promoter in both plasma and buffy coat. Hypermethylation of DAP-kinase promoter was found in both early- and late-stage NPC. Conclusions: Our results show that hypermethylation of the DAP-kinase promoter is a common early event in NPC. The high frequency of identification of hypermethylated DAP-kinase promoter in plasma and buffy coat of NPC patients illustrates its potential clinical application as tumor marker for the diagnosis and monitoring of treatment result.en_HK
dc.languageengen_HK
dc.publisherAmerican Association for Cancer Research.en_HK
dc.relation.ispartofClinical Cancer Researchen_HK
dc.subject.meshApoptosis Regulatory Proteinsen_HK
dc.subject.meshBiopsyen_HK
dc.subject.meshCalcium-Calmodulin-Dependent Protein Kinases - biosynthesis - blood - geneticsen_HK
dc.subject.meshCarcinoma - blood - enzymologyen_HK
dc.subject.meshDNA Methylationen_HK
dc.subject.meshFemaleen_HK
dc.subject.meshHumansen_HK
dc.subject.meshMaleen_HK
dc.subject.meshNasopharyngeal Neoplasms - blood - enzymologyen_HK
dc.subject.meshPromoter Regions, Geneticen_HK
dc.subject.meshSulfites - metabolismen_HK
dc.titleHigh frequency of promoter hypermethylation of the death-associated protein-kinase gene in nasopharyngeal carcinoma and its detection in the peripheral blood of patientsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1078-0432&volume=8&issue=2&spage=433&epage=437&date=2002&atitle=High+frequency+of+promoter+hypermethylation+of+the+death-associated+protein-kinase+gene+in+nasopharyngeal+carcinoma+and+its+detection+in+the+peripheral+blood+of+patientsen_HK
dc.identifier.emailThian, SW: thiansze@graduate.hku.hken_HK
dc.identifier.emailKwong, DLW: dlwkwong@hku.hken_HK
dc.identifier.authorityThian, SW=rp00478en_HK
dc.identifier.authorityKwong, DLW=rp00414en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.pmid11839660en_HK
dc.identifier.scopuseid_2-s2.0-0036190813en_HK
dc.identifier.hkuros65980en_HK
dc.identifier.hkuros142530-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0036190813&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume8en_HK
dc.identifier.issue2en_HK
dc.identifier.spage433en_HK
dc.identifier.epage437en_HK
dc.identifier.isiWOS:000173908600016-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridThian, SW=7403531328en_HK
dc.identifier.scopusauthoridHsiao, WC=7005796086en_HK
dc.identifier.scopusauthoridKwong, CT=7006566932en_HK
dc.identifier.scopusauthoridWilliam, IW=6506569969en_HK
dc.identifier.scopusauthoridKwong, DLW=15744231600en_HK
dc.identifier.scopusauthoridSham, JST=24472255400en_HK
dc.identifier.scopusauthoridYuen, APW=7006290111en_HK
dc.identifier.scopusauthoridYok, LK=6508259849en_HK

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