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Article: Comparative genomic hybridization analysis of genetic aberrations associated with development of esophageal squamous cell carcinoma in Henan, China

TitleComparative genomic hybridization analysis of genetic aberrations associated with development of esophageal squamous cell carcinoma in Henan, China
Authors
Issue Date2008
PublisherBaishideng Publishing Group. The Journal's web site is located at http://www.wjgnet.com/1007-9327/index.htm
Citation
World Journal Of Gastroenterology, 2008, v. 14 n. 12, p. 1828-1835 How to Cite?
AbstractAim: To characterize cytogenetic alterations in esophageal squamous cell carcinoma (ESCC) and its metastasis. Methods: A total of 37 cases of primary ESCC and 15 pairs of primary ESCC tumors and their matched metastatic lymph nodes cases were enrolled from Linzhou, the high incidence area for ESCC in Henan, northern China. The comparative genomic hybridization (CGH) was applied to determine the chromosomal aberrations on the DNA extracted from the frozen ESCC and metastatic lymph node samples from these patients. Results: CGH showed chromosomal aberrations in all the cases. In 37 cases of primary ESCC, chromosomal profile of DNA copy number was characterized by frequently detected gains at 8q (29/37, 78%), 3q (24/ 37, 65%), 5p (19/37, 51%); and frequently detected losses at 3p (21/37, 57%), 8p and 9q (14/37, 38%). In 15 pairs of primary ESCC tumors and their matched metastatic lymph node cases, the majority of the chromosomal aberrations in both primary tumor and metastatic lymph node lesions were consistent with the primary ESCC cases, but new candidate regions of interest were also detected. The most significant finding is the gains of chromosome 6p with a minimum high-level amplification region at 6p12-6q12 in 7 metastatic lymph nodes but only in 2 corresponding primary tumors (P = 0.05) and 20p with a minimum high-level amplification region at 20p12 in 11 metastatic lymph nodes but only in 5 corresponding primary tumors (P < 0.05). Another interesting finding is the loss of chromosome 10p and 10q in 8 and 7 metastatic lymph nodes but only in 2 corresponding primary tumors (P < 0.05). Conclusion: Using the CGH technique to detect chromosomal aberrations in both the primary tumor and its metastatic lymph nodes of ESCC, gains of 8q, 3q and 5p and loss of 3p, 8p, 9q and 13q were specifically implicated in ESCC in Linzhou population. Gains of 6p and 20p and loss of 10pq may contribute to the lymph node metastasis of ESCC. These findings suggest that the gains and losses of chromosomal regions may contain ESCC-related oncogenes and tumor suppressor genes and provide important theoretic information for identifying and cloning novel ESCC-related oncogenes and tumor suppressor genes. © 2008 WJG. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/72014
ISSN
2015 Impact Factor: 2.787
2015 SCImago Journal Rankings: 1.076
PubMed Central ID
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorQin, YRen_HK
dc.contributor.authorWang, LDen_HK
dc.contributor.authorFan, ZMen_HK
dc.contributor.authorKwong, Den_HK
dc.contributor.authorGuan, XYen_HK
dc.date.accessioned2010-09-06T06:37:30Z-
dc.date.available2010-09-06T06:37:30Z-
dc.date.issued2008en_HK
dc.identifier.citationWorld Journal Of Gastroenterology, 2008, v. 14 n. 12, p. 1828-1835en_HK
dc.identifier.issn1007-9327en_HK
dc.identifier.urihttp://hdl.handle.net/10722/72014-
dc.description.abstractAim: To characterize cytogenetic alterations in esophageal squamous cell carcinoma (ESCC) and its metastasis. Methods: A total of 37 cases of primary ESCC and 15 pairs of primary ESCC tumors and their matched metastatic lymph nodes cases were enrolled from Linzhou, the high incidence area for ESCC in Henan, northern China. The comparative genomic hybridization (CGH) was applied to determine the chromosomal aberrations on the DNA extracted from the frozen ESCC and metastatic lymph node samples from these patients. Results: CGH showed chromosomal aberrations in all the cases. In 37 cases of primary ESCC, chromosomal profile of DNA copy number was characterized by frequently detected gains at 8q (29/37, 78%), 3q (24/ 37, 65%), 5p (19/37, 51%); and frequently detected losses at 3p (21/37, 57%), 8p and 9q (14/37, 38%). In 15 pairs of primary ESCC tumors and their matched metastatic lymph node cases, the majority of the chromosomal aberrations in both primary tumor and metastatic lymph node lesions were consistent with the primary ESCC cases, but new candidate regions of interest were also detected. The most significant finding is the gains of chromosome 6p with a minimum high-level amplification region at 6p12-6q12 in 7 metastatic lymph nodes but only in 2 corresponding primary tumors (P = 0.05) and 20p with a minimum high-level amplification region at 20p12 in 11 metastatic lymph nodes but only in 5 corresponding primary tumors (P < 0.05). Another interesting finding is the loss of chromosome 10p and 10q in 8 and 7 metastatic lymph nodes but only in 2 corresponding primary tumors (P < 0.05). Conclusion: Using the CGH technique to detect chromosomal aberrations in both the primary tumor and its metastatic lymph nodes of ESCC, gains of 8q, 3q and 5p and loss of 3p, 8p, 9q and 13q were specifically implicated in ESCC in Linzhou population. Gains of 6p and 20p and loss of 10pq may contribute to the lymph node metastasis of ESCC. These findings suggest that the gains and losses of chromosomal regions may contain ESCC-related oncogenes and tumor suppressor genes and provide important theoretic information for identifying and cloning novel ESCC-related oncogenes and tumor suppressor genes. © 2008 WJG. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherBaishideng Publishing Group. The Journal's web site is located at http://www.wjgnet.com/1007-9327/index.htmen_HK
dc.relation.ispartofWorld Journal of Gastroenterologyen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subject.meshCarcinoma, Squamous Cell - genetics - pathologyen_HK
dc.subject.meshChinaen_HK
dc.subject.meshChromosome Aberrationsen_HK
dc.subject.meshChromosomes, Humanen_HK
dc.subject.meshCytogenetic Analysisen_HK
dc.subject.meshEsophageal Neoplasms - genetics - pathologyen_HK
dc.subject.meshHumansen_HK
dc.subject.meshLymph Nodes - pathologyen_HK
dc.subject.meshLymphatic Metastasisen_HK
dc.subject.meshNucleic Acid Hybridizationen_HK
dc.titleComparative genomic hybridization analysis of genetic aberrations associated with development of esophageal squamous cell carcinoma in Henan, Chinaen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1007-9327&volume=14&spage=1828&epage=1835&date=2008&atitle=Comparative+genomic+hybridization+analysis+of+genetic+aberrations+associated+with+development+of+esophageal+squamous+cell+carcinoma+in+Henan,+China.en_HK
dc.identifier.emailKwong, D:dlwkwong@hku.hken_HK
dc.identifier.emailGuan, XY:xyguan@hkucc.hku.hken_HK
dc.identifier.authorityKwong, D=rp00414en_HK
dc.identifier.authorityGuan, XY=rp00454en_HK
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.3748/wjg.14.1828en_HK
dc.identifier.pmid18350619en_HK
dc.identifier.pmcidPMC2700406-
dc.identifier.scopuseid_2-s2.0-42149174290en_HK
dc.identifier.hkuros146190en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-42149174290&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume14en_HK
dc.identifier.issue12en_HK
dc.identifier.spage1828en_HK
dc.identifier.epage1835en_HK
dc.identifier.isiWOS:000254407200005-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridQin, YR=7403100680en_HK
dc.identifier.scopusauthoridWang, LD=12242861000en_HK
dc.identifier.scopusauthoridFan, ZM=7402099547en_HK
dc.identifier.scopusauthoridKwong, D=15744231600en_HK
dc.identifier.scopusauthoridGuan, XY=7201463221en_HK

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