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Article: 1p31, 7q21 and 18q21 chromosomal aberrations and candidate genes in acquired vinblastine resistance of human cervical carcinoma KB cells

Title1p31, 7q21 and 18q21 chromosomal aberrations and candidate genes in acquired vinblastine resistance of human cervical carcinoma KB cells
Authors
Issue Date2008
PublisherDemetrios A Spandidos Ed & Pub. The Journal's web site is located at http://147.52.72.117/OR/or.htm
Citation
Oncology Reports, 2008, v. 19 n. 5, p. 1155-1164 How to Cite?
AbstractVinblastine (VBL) is used to treat certain kinds of ancer including Hodgkin's lymphoma, lung cancer, breast cancer, testicular cancer and cervical carcinoma. However, the rapid development of resistance during therapy remains a major clinical challenge. In order to reverse cancer cell resistance, the goal of this study was to find differentially expressed genes and chromosomal alterations in multidrug resistant (MDR) KB-v1 cells, further to probe the relationship between drug resistance and differential genes, and chromosomal changes in MDR cancer cells. Comparative genomic hybridization (CGH) analysis of MDR KB-v1 and their parental KB-3-1 cells revealed chromosomal changes; microarray-based expression profiling was carried out by comparing the gene expressions of MDR KB-v1 cells and KB-3-1 cells. We have identified 3 chromosomal gains in regions of 1p31, 7q21 and 18q21 in MDR cells and 10 genes (CYR61, UGTREL7, MBD1, NARS, ATP5A1, ABCB1, ABCB4, PEG10, MCM7, SERPINE1) contained in these regions were also up-regulated in MDR KB-v1 cells. Forty-nine genes were down-regulated when KB-v1 cells were subjected to lower dose or depletion of the drug. We have confirmed some gene expression changes by reverse transcription-polymerase chain reaction and Northern blots. These are the first data describing the relationship of 1p31 and 18q21 chromosomal aberrations and candidate genes in acquired vinblastine-resistance. This study also demonstrates that the combination of CGH and cDNA microarray is a very useful tool to detect drug resistant targets in cancer treatment.
Persistent Identifierhttp://hdl.handle.net/10722/72013
ISSN
2015 Impact Factor: 2.486
2015 SCImago Journal Rankings: 0.968
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorWang, Jen_HK
dc.contributor.authorTai, LSen_HK
dc.contributor.authorTzang, CHen_HK
dc.contributor.authorFong, WFen_HK
dc.contributor.authorGuan, XYen_HK
dc.contributor.authorYang, Men_HK
dc.date.accessioned2010-09-06T06:37:29Z-
dc.date.available2010-09-06T06:37:29Z-
dc.date.issued2008en_HK
dc.identifier.citationOncology Reports, 2008, v. 19 n. 5, p. 1155-1164en_HK
dc.identifier.issn1021-335Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/72013-
dc.description.abstractVinblastine (VBL) is used to treat certain kinds of ancer including Hodgkin's lymphoma, lung cancer, breast cancer, testicular cancer and cervical carcinoma. However, the rapid development of resistance during therapy remains a major clinical challenge. In order to reverse cancer cell resistance, the goal of this study was to find differentially expressed genes and chromosomal alterations in multidrug resistant (MDR) KB-v1 cells, further to probe the relationship between drug resistance and differential genes, and chromosomal changes in MDR cancer cells. Comparative genomic hybridization (CGH) analysis of MDR KB-v1 and their parental KB-3-1 cells revealed chromosomal changes; microarray-based expression profiling was carried out by comparing the gene expressions of MDR KB-v1 cells and KB-3-1 cells. We have identified 3 chromosomal gains in regions of 1p31, 7q21 and 18q21 in MDR cells and 10 genes (CYR61, UGTREL7, MBD1, NARS, ATP5A1, ABCB1, ABCB4, PEG10, MCM7, SERPINE1) contained in these regions were also up-regulated in MDR KB-v1 cells. Forty-nine genes were down-regulated when KB-v1 cells were subjected to lower dose or depletion of the drug. We have confirmed some gene expression changes by reverse transcription-polymerase chain reaction and Northern blots. These are the first data describing the relationship of 1p31 and 18q21 chromosomal aberrations and candidate genes in acquired vinblastine-resistance. This study also demonstrates that the combination of CGH and cDNA microarray is a very useful tool to detect drug resistant targets in cancer treatment.en_HK
dc.languageengen_HK
dc.publisherDemetrios A Spandidos Ed & Pub. The Journal's web site is located at http://147.52.72.117/OR/or.htmen_HK
dc.relation.ispartofOncology Reportsen_HK
dc.subject.meshAntineoplastic Agents - pharmacologyen_HK
dc.subject.meshCell Cycleen_HK
dc.subject.meshCell Line, Tumoren_HK
dc.subject.meshChromosome Aberrationsen_HK
dc.subject.meshChromosomes, Human, Pair 1en_HK
dc.subject.meshChromosomes, Human, Pair 18en_HK
dc.subject.meshChromosomes, Human, Pair 7en_HK
dc.subject.meshDrug Resistance, Neoplasmen_HK
dc.subject.meshFemaleen_HK
dc.subject.meshGene Expression Regulation, Neoplasticen_HK
dc.subject.meshHumansen_HK
dc.subject.meshKB Cellsen_HK
dc.subject.meshNucleic Acid Hybridizationen_HK
dc.subject.meshUterine Cervical Neoplasms - geneticsen_HK
dc.subject.meshVinblastine - pharmacologyen_HK
dc.title1p31, 7q21 and 18q21 chromosomal aberrations and candidate genes in acquired vinblastine resistance of human cervical carcinoma KB cellsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1021-335X&volume=19&spage=1155&epage=1164&date=2008&atitle=1p31,+7q21+and+18q21+chromosomal+aberrations+and+candidate+genes+in+acquired+vinblastine+resistance+of+human+cervical+carcinoma+KB+cells.en_HK
dc.identifier.emailGuan, XY:xyguan@hkucc.hku.hken_HK
dc.identifier.authorityGuan, XY=rp00454en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.pmid18425371-
dc.identifier.scopuseid_2-s2.0-47549107093en_HK
dc.identifier.hkuros146187en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-47549107093&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume19en_HK
dc.identifier.issue5en_HK
dc.identifier.spage1155en_HK
dc.identifier.epage1164en_HK
dc.identifier.isiWOS:000255312000012-
dc.publisher.placeGreeceen_HK
dc.identifier.scopusauthoridWang, J=7701314990en_HK
dc.identifier.scopusauthoridTai, LS=7004457333en_HK
dc.identifier.scopusauthoridTzang, CH=6508203245en_HK
dc.identifier.scopusauthoridFong, WF=24471508200en_HK
dc.identifier.scopusauthoridGuan, XY=7201463221en_HK
dc.identifier.scopusauthoridYang, M=35204210300en_HK

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