File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: The effects of various irradiation doses on the growth and differentiation of marrow-derived human mesenchymal stromal cells

TitleThe effects of various irradiation doses on the growth and differentiation of marrow-derived human mesenchymal stromal cells
Authors
KeywordsDifferentiation
Irradiation
Mesenchymal stromal cells
Proliferation
Retinoic acid
Issue Date2007
PublisherBlackwell Munksgaard. The Journal's web site is located at http://www.blackwellpublishing.com/journals/PTR
Citation
Pediatric Transplantation, 2007, v. 11 n. 4, p. 379-387 How to Cite?
AbstractMesenchymal stromal cells (MSC) are progenitors of mesenchymal tissues including bones. Irradiation can damage the osteogenic activity of human marrow by suppressing osteoblasts leading to post-irradiation osteoporosis. However, the effect of therapeutic irradiation on MSC remains unexplored. We investigated the effects of various doses of X-ray irradiation on human MSC (hMSC) by measuring its post-irradiation proliferation and differentiation activities. Standard immunophenotypes and differentiating functions of the MSC were determined. Irradiation inhibited proliferation of hMSC up to two wk post-irradiation but thereafter, those residual surviving cells regained their normal proliferation rate. Bone forming activity as reflected by alkaline phosphatase (ALP) and calcium deposition were both reduced in a dose-dependent fashion. Maximum suppressive effect on osteogenic activity was noted in MSC treated with high-dose irradiation (12 Gy). Adipocyte percentage was also reduced by 50% in cultures that received >4 Gy. Attempts to protect the irradiated cells with 1 μm all-trans retinoic acid did not show any beneficial effect on MSC proliferation and differentiation. The direct impairment of proliferation and osteogenic differentiation potential of MSC by irradiation may contribute partly to the post-transplant osteoporosis. © 2007 Blackwell Munksgaard.
Persistent Identifierhttp://hdl.handle.net/10722/71986
ISSN
2015 Impact Factor: 1.284
2015 SCImago Journal Rankings: 0.542
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLi, Jen_HK
dc.contributor.authorKwong, DLWen_HK
dc.contributor.authorChan, GCFen_HK
dc.date.accessioned2010-09-06T06:37:11Z-
dc.date.available2010-09-06T06:37:11Z-
dc.date.issued2007en_HK
dc.identifier.citationPediatric Transplantation, 2007, v. 11 n. 4, p. 379-387en_HK
dc.identifier.issn1397-3142en_HK
dc.identifier.urihttp://hdl.handle.net/10722/71986-
dc.description.abstractMesenchymal stromal cells (MSC) are progenitors of mesenchymal tissues including bones. Irradiation can damage the osteogenic activity of human marrow by suppressing osteoblasts leading to post-irradiation osteoporosis. However, the effect of therapeutic irradiation on MSC remains unexplored. We investigated the effects of various doses of X-ray irradiation on human MSC (hMSC) by measuring its post-irradiation proliferation and differentiation activities. Standard immunophenotypes and differentiating functions of the MSC were determined. Irradiation inhibited proliferation of hMSC up to two wk post-irradiation but thereafter, those residual surviving cells regained their normal proliferation rate. Bone forming activity as reflected by alkaline phosphatase (ALP) and calcium deposition were both reduced in a dose-dependent fashion. Maximum suppressive effect on osteogenic activity was noted in MSC treated with high-dose irradiation (12 Gy). Adipocyte percentage was also reduced by 50% in cultures that received >4 Gy. Attempts to protect the irradiated cells with 1 μm all-trans retinoic acid did not show any beneficial effect on MSC proliferation and differentiation. The direct impairment of proliferation and osteogenic differentiation potential of MSC by irradiation may contribute partly to the post-transplant osteoporosis. © 2007 Blackwell Munksgaard.en_HK
dc.languageengen_HK
dc.publisherBlackwell Munksgaard. The Journal's web site is located at http://www.blackwellpublishing.com/journals/PTRen_HK
dc.relation.ispartofPediatric Transplantationen_HK
dc.subjectDifferentiationen_HK
dc.subjectIrradiationen_HK
dc.subjectMesenchymal stromal cellsen_HK
dc.subjectProliferationen_HK
dc.subjectRetinoic aciden_HK
dc.subject.meshAdipocytes - cytology - drug effects - radiation effectsen_HK
dc.subject.meshAdipogenesis - radiation effectsen_HK
dc.subject.meshAlkaline Phosphatase - metabolismen_HK
dc.subject.meshBone Marrow Cells - cytology - drug effects - radiation effectsen_HK
dc.subject.meshBone and Bones - drug effects - metabolism - radiation effectsen_HK
dc.subject.meshCalcium - metabolismen_HK
dc.subject.meshCell Differentiation - drug effects - radiation effectsen_HK
dc.subject.meshCell Proliferation - drug effects - radiation effectsen_HK
dc.subject.meshCells, Cultureden_HK
dc.subject.meshDose-Response Relationship, Radiationen_HK
dc.subject.meshHumansen_HK
dc.subject.meshKeratolytic Agents - pharmacologyen_HK
dc.subject.meshMesenchymal Stem Cell Transplantationen_HK
dc.subject.meshMesenchymal Stem Cells - cytology - drug effects - radiation effectsen_HK
dc.subject.meshOsteogenesis - drug effects - radiation effectsen_HK
dc.subject.meshRadiation Injuries - prevention & controlen_HK
dc.subject.meshTretinoin - pharmacologyen_HK
dc.subject.meshX-Raysen_HK
dc.titleThe effects of various irradiation doses on the growth and differentiation of marrow-derived human mesenchymal stromal cellsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1397-3142&volume=11&spage=379&epage=387&date=2007&atitle=The+effects+of+various+irradiation+doses+on+the+growth+and+differentiation+of+marrow-derived+human+mesenchymal+stromal+cellsen_HK
dc.identifier.emailKwong, DLW:dlwkwong@hku.hken_HK
dc.identifier.emailChan, GCF:gcfchan@hkucc.hku.hken_HK
dc.identifier.authorityKwong, DLW=rp00414en_HK
dc.identifier.authorityChan, GCF=rp00431en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1111/j.1399-3046.2006.00663.xen_HK
dc.identifier.pmid17493217-
dc.identifier.scopuseid_2-s2.0-34247587315en_HK
dc.identifier.hkuros126938en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-34247587315&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume11en_HK
dc.identifier.issue4en_HK
dc.identifier.spage379en_HK
dc.identifier.epage387en_HK
dc.identifier.isiWOS:000246040100006-
dc.publisher.placeDenmarken_HK
dc.identifier.scopusauthoridLi, J=26650453600en_HK
dc.identifier.scopusauthoridKwong, DLW=15744231600en_HK
dc.identifier.scopusauthoridChan, GCF=16160154400en_HK
dc.identifier.citeulike1265441-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats