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Article: Clusterin plays an important role in hepatocellular carcinoma metastasis

TitleClusterin plays an important role in hepatocellular carcinoma metastasis
Authors
Issue Date2006
PublisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/onc
Citation
Oncogene, 2006, v. 25 n. 8, p. 1242-1250 How to Cite?
AbstractTo identify genes associated with tumor metastasis in hepatocellular carcinoma (HCC), gene expression profiles between a pair of primary HCC (H2-P) and their matched metastatic HCC (H2-M) were compared. Overexpression of clusterin (CLU) was found in H2-M cells. To determine the roles CLU played in HCC metastasis, CLU was transfected into H2-P cells. Overexpression of CLU in H2-P cells increased cell migration by twofold in vitro and formation of metastatic tumor nodules in liver by eightfold in vivo. To evaluate the correlation of CLU expression with HCC metastasis, the expression levels of CLU in HCCs were investigated using a tissue microarray (TMA) containing 104 pairs of primary HCCs and their matched metastases. The frequency of CLU overexpression increased significantly in metastatic HCCs (59.1%) compared with that in primary tumors (32.6%, P < 0.001). To gain additional insight into the function of CLU, the expression profile of H2P-CLU was compared with vector-transfected H2-P cells by cDNA microarray. A total of 35 upregulated and 14 downregulated genes were detected in H2P-CLU. One of the upregulated genes known as YKL-40, which is implicated in matrix-remodeling and metastasis, was further studied using TMA. A significant correlation (P < 0.001) between the expression levels of YKL-40 and CLU was observed, implying that the CLU-YKL-40 pathway may play an important role in HCC metastasis. © 2006 Nature Publishing Group. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/71949
ISSN
2015 Impact Factor: 7.932
2015 SCImago Journal Rankings: 4.047
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLau, SHen_HK
dc.contributor.authorSham, JSTen_HK
dc.contributor.authorXie, Den_HK
dc.contributor.authorTzang, CHen_HK
dc.contributor.authorTang, Den_HK
dc.contributor.authorMa, Nen_HK
dc.contributor.authorHu, Len_HK
dc.contributor.authorWang, Yen_HK
dc.contributor.authorWen, JMen_HK
dc.contributor.authorXiao, Gen_HK
dc.contributor.authorZhang, WMen_HK
dc.contributor.authorLau, GKKen_HK
dc.contributor.authorYang, Men_HK
dc.contributor.authorGuan, XYen_HK
dc.date.accessioned2010-09-06T06:36:48Z-
dc.date.available2010-09-06T06:36:48Z-
dc.date.issued2006en_HK
dc.identifier.citationOncogene, 2006, v. 25 n. 8, p. 1242-1250en_HK
dc.identifier.issn0950-9232en_HK
dc.identifier.urihttp://hdl.handle.net/10722/71949-
dc.description.abstractTo identify genes associated with tumor metastasis in hepatocellular carcinoma (HCC), gene expression profiles between a pair of primary HCC (H2-P) and their matched metastatic HCC (H2-M) were compared. Overexpression of clusterin (CLU) was found in H2-M cells. To determine the roles CLU played in HCC metastasis, CLU was transfected into H2-P cells. Overexpression of CLU in H2-P cells increased cell migration by twofold in vitro and formation of metastatic tumor nodules in liver by eightfold in vivo. To evaluate the correlation of CLU expression with HCC metastasis, the expression levels of CLU in HCCs were investigated using a tissue microarray (TMA) containing 104 pairs of primary HCCs and their matched metastases. The frequency of CLU overexpression increased significantly in metastatic HCCs (59.1%) compared with that in primary tumors (32.6%, P < 0.001). To gain additional insight into the function of CLU, the expression profile of H2P-CLU was compared with vector-transfected H2-P cells by cDNA microarray. A total of 35 upregulated and 14 downregulated genes were detected in H2P-CLU. One of the upregulated genes known as YKL-40, which is implicated in matrix-remodeling and metastasis, was further studied using TMA. A significant correlation (P < 0.001) between the expression levels of YKL-40 and CLU was observed, implying that the CLU-YKL-40 pathway may play an important role in HCC metastasis. © 2006 Nature Publishing Group. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/oncen_HK
dc.relation.ispartofOncogeneen_HK
dc.subject.meshAdipokinesen_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshBone Neoplasms - metabolism - secondaryen_HK
dc.subject.meshCarcinoma, Hepatocellular - metabolism - secondaryen_HK
dc.subject.meshCell Movementen_HK
dc.subject.meshClusterin - metabolismen_HK
dc.subject.meshFemaleen_HK
dc.subject.meshGene Expression Profilingen_HK
dc.subject.meshGlycoproteins - metabolismen_HK
dc.subject.meshHumansen_HK
dc.subject.meshKidney Neoplasms - metabolism - secondaryen_HK
dc.subject.meshLectinsen_HK
dc.subject.meshLiver Neoplasms, Experimental - metabolism - pathologyen_HK
dc.subject.meshLymphatic Metastasis - pathologyen_HK
dc.subject.meshMiceen_HK
dc.subject.meshMice, SCIDen_HK
dc.subject.meshOligonucleotide Array Sequence Analysisen_HK
dc.subject.meshTissue Array Analysisen_HK
dc.subject.meshTumor Markers, Biologicalen_HK
dc.titleClusterin plays an important role in hepatocellular carcinoma metastasisen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0950-9232&volume=25&spage=1242&epage=1250&date=2006&atitle=Clusterin+plays+an+important+role+in+hepatocellular+carcinoma+metastasis.en_HK
dc.identifier.emailGuan, XY:xyguan@hkucc.hku.hken_HK
dc.identifier.authorityGuan, XY=rp00454en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1038/sj.onc.1209141en_HK
dc.identifier.pmid16247463-
dc.identifier.scopuseid_2-s2.0-33644518424en_HK
dc.identifier.hkuros119637en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-33644518424&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume25en_HK
dc.identifier.issue8en_HK
dc.identifier.spage1242en_HK
dc.identifier.epage1250en_HK
dc.identifier.isiWOS:000235537500011-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridLau, SH=36194005500en_HK
dc.identifier.scopusauthoridSham, JST=7101655565en_HK
dc.identifier.scopusauthoridXie, D=35070710200en_HK
dc.identifier.scopusauthoridTzang, CH=6508203245en_HK
dc.identifier.scopusauthoridTang, D=12752134500en_HK
dc.identifier.scopusauthoridMa, N=35731661400en_HK
dc.identifier.scopusauthoridHu, L=34770075600en_HK
dc.identifier.scopusauthoridWang, Y=7601486269en_HK
dc.identifier.scopusauthoridWen, JM=7402701931en_HK
dc.identifier.scopusauthoridXiao, G=49764056900en_HK
dc.identifier.scopusauthoridZhang, WM=12646682300en_HK
dc.identifier.scopusauthoridLau, GKK=7102301257en_HK
dc.identifier.scopusauthoridYang, M=7404925734en_HK
dc.identifier.scopusauthoridGuan, XY=7201463221en_HK
dc.identifier.citeulike462007-

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