Article: Analysis of genetic alterations in primary nasopharyngeal carcinoma by comparative genomic hybridization

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TitleAnalysis of genetic alterations in primary nasopharyngeal carcinoma by comparative genomic hybridization
AuthorsFang, Y3
Guan, XY2
Guo, Y
Sham, JST2
Deng, M2
Liang, Q1
Li, H3
Zhang, H1 2
Zhou, H1
Trent, J2
Issue Date2001
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/38250
CitationGenes Chromosomes And Cancer, 2001, v. 30 n. 3, p. 254-260 [How to Cite?]
DOI: http://dx.doi.org/10.1002/1098-2264(2000)9999:9999<::AID-GCC1086>3.0.CO;2-D
AbstractTo identify genetic alterations associated with the development and progression of human nasopharyngeal carcinoma (NPC), 57 tumors were analyzed by comparative genomic hybridization (CGH). In 47 cases, chromosomal imbalances were found. Several recurrent chromosomal abnormalities were identified in the present study. The most frequently detected chromosomal gains involved chromosome arms 12q (24 cases, 51%), 4q (17 cases, 36%), 3q (16 cases, 34%), 1q (15 cases, 32%), and 18q (15 cases, 32%). Common regions of gain involved 12q13-q15, 4q12-q21, and 3q21-q26. High-copy-number increases of chromosomal materials were detected in four chromosomal regions, 3q21-q26.2, 4p12-q21, 8p, and 12q14-q15. The most frequently detected loss of chromosomal materials involved chromosome arms 16q (26 cases, 55%), 14q (21 cases, 45%), 1p (20 cases, 43%), 3p (20 cases, 43%), 16p (19 cases, 40%), 11q (17 cases, 36%), and 19p (16 cases, 34%). The most common regions of loss involved 14q24-qter, 1pter-p36.1, 3p22-p21.3, 11q21-qter, and the distal region of 19p. Genomic alterations detected by CGH were compared and found to be largely consistent with those identified in banding analysis and loss of heterozygosity studies. However, several previously unrecognized recurrent alterations were also identified in the present study, including gain of 4q and 18q, and loss of 16q, 14q, and 19p. In addition, gain of 1q, 8q, 18q, and loss of 9q showed a statistically significant association with advanced clinical stages (P < 0.05). Identification of recurrent sites of chromosomal gain and loss identify regions of the genome that may contain oncogenes or tumor suppressor genes, respectively, which may be involved in the tumorigenesis of NPC.
ISSN1045-2257
2011 Impact Factor: 3.306
2011 SCImago Journal Rankings: 0.633
DOIhttp://dx.doi.org/10.1002/1098-2264(2000)9999:9999<::AID-GCC1086>3.0.CO;2-D
ISI Accession Number IDWOS:000166849300005
ReferencesReferences in Scopus
DC Field
Value
dc.contributor.authorFang, Y
dc.contributor.authorGuan, XY
dc.contributor.authorGuo, Y
dc.contributor.authorSham, JST
dc.contributor.authorDeng, M
dc.contributor.authorLiang, Q
dc.contributor.authorLi, H
dc.contributor.authorZhang, H
dc.contributor.authorZhou, H
dc.contributor.authorTrent, J
dc.date.accessioned2010-09-06T06:36:43Z
dc.date.available2010-09-06T06:36:43Z
dc.date.issued2001
dc.description.abstractTo identify genetic alterations associated with the development and progression of human nasopharyngeal carcinoma (NPC), 57 tumors were analyzed by comparative genomic hybridization (CGH). In 47 cases, chromosomal imbalances were found. Several recurrent chromosomal abnormalities were identified in the present study. The most frequently detected chromosomal gains involved chromosome arms 12q (24 cases, 51%), 4q (17 cases, 36%), 3q (16 cases, 34%), 1q (15 cases, 32%), and 18q (15 cases, 32%). Common regions of gain involved 12q13-q15, 4q12-q21, and 3q21-q26. High-copy-number increases of chromosomal materials were detected in four chromosomal regions, 3q21-q26.2, 4p12-q21, 8p, and 12q14-q15. The most frequently detected loss of chromosomal materials involved chromosome arms 16q (26 cases, 55%), 14q (21 cases, 45%), 1p (20 cases, 43%), 3p (20 cases, 43%), 16p (19 cases, 40%), 11q (17 cases, 36%), and 19p (16 cases, 34%). The most common regions of loss involved 14q24-qter, 1pter-p36.1, 3p22-p21.3, 11q21-qter, and the distal region of 19p. Genomic alterations detected by CGH were compared and found to be largely consistent with those identified in banding analysis and loss of heterozygosity studies. However, several previously unrecognized recurrent alterations were also identified in the present study, including gain of 4q and 18q, and loss of 16q, 14q, and 19p. In addition, gain of 1q, 8q, 18q, and loss of 9q showed a statistically significant association with advanced clinical stages (P < 0.05). Identification of recurrent sites of chromosomal gain and loss identify regions of the genome that may contain oncogenes or tumor suppressor genes, respectively, which may be involved in the tumorigenesis of NPC.
dc.description.natureLink_to_subscribed_fulltext
dc.identifier.citationGenes Chromosomes And Cancer, 2001, v. 30 n. 3, p. 254-260 [How to Cite?]
DOI: http://dx.doi.org/10.1002/1098-2264(2000)9999:9999<::AID-GCC1086>3.0.CO;2-D
dc.identifier.doihttp://dx.doi.org/10.1002/1098-2264(2000)9999:9999<::AID-GCC1086>3.0.CO;2-D
dc.identifier.epage260
dc.identifier.hkuros100395
dc.identifier.isiWOS:000166849300005
dc.identifier.issn1045-2257
2011 Impact Factor: 3.306
2011 SCImago Journal Rankings: 0.633
dc.identifier.issue3
dc.identifier.openurl
dc.identifier.pmid11170282
dc.identifier.scopuseid_2-s2.0-0035154261
dc.identifier.spage254
dc.identifier.urihttp://hdl.handle.net/10722/71940
dc.identifier.volume30
dc.languageeng
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/38250
dc.publisher.placeUnited States
dc.relation.ispartofGenes Chromosomes and Cancer
dc.relation.referencesReferences in Scopus
dc.rightsGenes, Chromosomes & Cancer. Copyright © John Wiley & Sons, Inc.
dc.subject.meshAdult
dc.subject.meshAged
dc.subject.meshCarcinoma, Squamous Cell - genetics
dc.subject.meshChromosome Aberrations - genetics
dc.subject.meshChromosome Deletion
dc.subject.meshChromosome Disorders
dc.subject.meshDNA, Neoplasm - analysis
dc.subject.meshFemale
dc.subject.meshHumans
dc.subject.meshMale
dc.subject.meshMiddle Aged
dc.subject.meshNasopharyngeal Neoplasms - genetics
dc.subject.meshNucleic Acid Hybridization - methods
dc.titleAnalysis of genetic alterations in primary nasopharyngeal carcinoma by comparative genomic hybridization
dc.typeArticle
Author Affiliations
  1. National Human Genome Research Institute
  2. The University of Hong Kong
  3. Sun Yat Sen University of Medical Sciences