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Article: Characterization of 3p, 5p, and 3q in two nasopharyngeal carcinoma cell lines, using region-specific multiplex fluorescence in situ hybridization probes
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TitleCharacterization of 3p, 5p, and 3q in two nasopharyngeal carcinoma cell lines, using region-specific multiplex fluorescence in situ hybridization probes
 
AuthorsTjia, WM1
Sham, JST1
Hu, L1
Tai, ALS1
Guan, XY1
 
Issue Date2005
 
PublisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/cancergene
 
CitationCancer Genetics And Cytogenetics, 2005, v. 158 n. 1, p. 61-66 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.cancergencyto.2004.08.024
 
AbstractAmplification of chromosome arms 3q and 5p and deletion of 3p were frequently detected in nasopharyngeal carcinoma (NPC) with comparative genomic hybridization and loss of heterozygosity studies. To identify the minimal amplified or deleted regions in these arms, structural aberrations in chromosome arms 3p, 3q, and 5p in two NPC cell lines, CNE1 and SUNE1, were studied with multiplex-color FISH (M-FISH) and chromosome region-specific probes (CRP). All CRPs, which were generated from microdissected DNA, were specific and strong in intensity, and sensitive enough to detect chromosome aberrations including translocations, deletions, and amplifications of target regions. In these two NPC cell lines, minimal regions of deletion and amplification were found at 3p12 and 3q26∼q27, respectively. On 5p, most of the regions were amplified as intact copies. Interregion translocations of these three arms were also observed. The amplification on 3q26∼q27 provided useful hints for further screening the minimal amplification at RP11-115J24 (3q26.2), containing candidate oncogene eIF-5A2. M-FISH with CRPs is thus not only useful in revealing a comprehensive picture of structural aberrations in target chromosomes, but also in narrowing down the minimal region for screening cancer-related genes. © 2005 Elsevier Inc. All rights reserved.
 
ISSN0165-4608
 
DOIhttp://dx.doi.org/10.1016/j.cancergencyto.2004.08.024
 
ISI Accession Number IDWOS:000228117500007
 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorTjia, WM
 
dc.contributor.authorSham, JST
 
dc.contributor.authorHu, L
 
dc.contributor.authorTai, ALS
 
dc.contributor.authorGuan, XY
 
dc.date.accessioned2010-09-06T06:36:33Z
 
dc.date.available2010-09-06T06:36:33Z
 
dc.date.issued2005
 
dc.description.abstractAmplification of chromosome arms 3q and 5p and deletion of 3p were frequently detected in nasopharyngeal carcinoma (NPC) with comparative genomic hybridization and loss of heterozygosity studies. To identify the minimal amplified or deleted regions in these arms, structural aberrations in chromosome arms 3p, 3q, and 5p in two NPC cell lines, CNE1 and SUNE1, were studied with multiplex-color FISH (M-FISH) and chromosome region-specific probes (CRP). All CRPs, which were generated from microdissected DNA, were specific and strong in intensity, and sensitive enough to detect chromosome aberrations including translocations, deletions, and amplifications of target regions. In these two NPC cell lines, minimal regions of deletion and amplification were found at 3p12 and 3q26∼q27, respectively. On 5p, most of the regions were amplified as intact copies. Interregion translocations of these three arms were also observed. The amplification on 3q26∼q27 provided useful hints for further screening the minimal amplification at RP11-115J24 (3q26.2), containing candidate oncogene eIF-5A2. M-FISH with CRPs is thus not only useful in revealing a comprehensive picture of structural aberrations in target chromosomes, but also in narrowing down the minimal region for screening cancer-related genes. © 2005 Elsevier Inc. All rights reserved.
 
dc.description.naturelink_to_subscribed_fulltext
 
dc.identifier.citationCancer Genetics And Cytogenetics, 2005, v. 158 n. 1, p. 61-66 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.cancergencyto.2004.08.024
 
dc.identifier.doihttp://dx.doi.org/10.1016/j.cancergencyto.2004.08.024
 
dc.identifier.epage66
 
dc.identifier.hkuros115338
 
dc.identifier.isiWOS:000228117500007
 
dc.identifier.issn0165-4608
 
dc.identifier.issue1
 
dc.identifier.openurl
 
dc.identifier.pmid15771906
 
dc.identifier.scopuseid_2-s2.0-14844355856
 
dc.identifier.spage61
 
dc.identifier.urihttp://hdl.handle.net/10722/71924
 
dc.identifier.volume158
 
dc.languageeng
 
dc.publisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/cancergene
 
dc.publisher.placeUnited States
 
dc.relation.ispartofCancer Genetics and Cytogenetics
 
dc.relation.referencesReferences in Scopus
 
dc.rightsCancer Genetics and Cytogenetics. Copyright © Elsevier Inc.
 
dc.subject.meshCell Line, Tumor
 
dc.subject.meshChromosome Aberrations
 
dc.subject.meshChromosomes, Human, Pair 3
 
dc.subject.meshChromosomes, Human, Pair 5
 
dc.subject.meshGenetic Markers
 
dc.subject.meshHumans
 
dc.subject.meshIn Situ Hybridization, Fluorescence
 
dc.subject.meshMolecular Probes
 
dc.subject.meshNasopharyngeal Neoplasms - genetics - pathology
 
dc.titleCharacterization of 3p, 5p, and 3q in two nasopharyngeal carcinoma cell lines, using region-specific multiplex fluorescence in situ hybridization probes
 
dc.typeArticle
 
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Author Affiliations
  1. The University of Hong Kong