Article: Characterization of 3p, 5p, and 3q in two nasopharyngeal carcinoma cell lines, using region-specific multiplex fluorescence in situ hybridization probes

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TitleCharacterization of 3p, 5p, and 3q in two nasopharyngeal carcinoma cell lines, using region-specific multiplex fluorescence in situ hybridization probes
AuthorsTjia, WM1
Sham, JST1
Hu, L1
Tai, ALS1
Guan, XY1
Issue Date2005
PublisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/cancergene
CitationCancer Genetics And Cytogenetics, 2005, v. 158 n. 1, p. 61-66 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.cancergencyto.2004.08.024
AbstractAmplification of chromosome arms 3q and 5p and deletion of 3p were frequently detected in nasopharyngeal carcinoma (NPC) with comparative genomic hybridization and loss of heterozygosity studies. To identify the minimal amplified or deleted regions in these arms, structural aberrations in chromosome arms 3p, 3q, and 5p in two NPC cell lines, CNE1 and SUNE1, were studied with multiplex-color FISH (M-FISH) and chromosome region-specific probes (CRP). All CRPs, which were generated from microdissected DNA, were specific and strong in intensity, and sensitive enough to detect chromosome aberrations including translocations, deletions, and amplifications of target regions. In these two NPC cell lines, minimal regions of deletion and amplification were found at 3p12 and 3q26∼q27, respectively. On 5p, most of the regions were amplified as intact copies. Interregion translocations of these three arms were also observed. The amplification on 3q26∼q27 provided useful hints for further screening the minimal amplification at RP11-115J24 (3q26.2), containing candidate oncogene eIF-5A2. M-FISH with CRPs is thus not only useful in revealing a comprehensive picture of structural aberrations in target chromosomes, but also in narrowing down the minimal region for screening cancer-related genes. © 2005 Elsevier Inc. All rights reserved.
ISSN0165-4608
2011 Impact Factor: 1.389
2011 SCImago Journal Rankings: 0.162
DOIhttp://dx.doi.org/10.1016/j.cancergencyto.2004.08.024
ISI Accession Number IDWOS:000228117500007
ReferencesReferences in Scopus
DC Field
Value
dc.contributor.authorTjia, WM
dc.contributor.authorSham, JST
dc.contributor.authorHu, L
dc.contributor.authorTai, ALS
dc.contributor.authorGuan, XY
dc.date.accessioned2010-09-06T06:36:33Z
dc.date.available2010-09-06T06:36:33Z
dc.date.issued2005
dc.description.abstractAmplification of chromosome arms 3q and 5p and deletion of 3p were frequently detected in nasopharyngeal carcinoma (NPC) with comparative genomic hybridization and loss of heterozygosity studies. To identify the minimal amplified or deleted regions in these arms, structural aberrations in chromosome arms 3p, 3q, and 5p in two NPC cell lines, CNE1 and SUNE1, were studied with multiplex-color FISH (M-FISH) and chromosome region-specific probes (CRP). All CRPs, which were generated from microdissected DNA, were specific and strong in intensity, and sensitive enough to detect chromosome aberrations including translocations, deletions, and amplifications of target regions. In these two NPC cell lines, minimal regions of deletion and amplification were found at 3p12 and 3q26∼q27, respectively. On 5p, most of the regions were amplified as intact copies. Interregion translocations of these three arms were also observed. The amplification on 3q26∼q27 provided useful hints for further screening the minimal amplification at RP11-115J24 (3q26.2), containing candidate oncogene eIF-5A2. M-FISH with CRPs is thus not only useful in revealing a comprehensive picture of structural aberrations in target chromosomes, but also in narrowing down the minimal region for screening cancer-related genes. © 2005 Elsevier Inc. All rights reserved.
dc.description.natureLink_to_subscribed_fulltext
dc.identifier.citationCancer Genetics And Cytogenetics, 2005, v. 158 n. 1, p. 61-66 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.cancergencyto.2004.08.024
dc.identifier.doihttp://dx.doi.org/10.1016/j.cancergencyto.2004.08.024
dc.identifier.epage66
dc.identifier.hkuros115338
dc.identifier.isiWOS:000228117500007
dc.identifier.issn0165-4608
2011 Impact Factor: 1.389
2011 SCImago Journal Rankings: 0.162
dc.identifier.issue1
dc.identifier.openurl
dc.identifier.pmid15771906
dc.identifier.scopuseid_2-s2.0-14844355856
dc.identifier.spage61
dc.identifier.urihttp://hdl.handle.net/10722/71924
dc.identifier.volume158
dc.languageeng
dc.publisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/cancergene
dc.publisher.placeUnited States
dc.relation.ispartofCancer Genetics and Cytogenetics
dc.relation.referencesReferences in Scopus
dc.rightsCancer Genetics and Cytogenetics. Copyright © Elsevier Inc.
dc.subject.meshCell Line, Tumor
dc.subject.meshChromosome Aberrations
dc.subject.meshChromosomes, Human, Pair 3
dc.subject.meshChromosomes, Human, Pair 5
dc.subject.meshGenetic Markers
dc.subject.meshHumans
dc.subject.meshIn Situ Hybridization, Fluorescence
dc.subject.meshMolecular Probes
dc.subject.meshNasopharyngeal Neoplasms - genetics - pathology
dc.titleCharacterization of 3p, 5p, and 3q in two nasopharyngeal carcinoma cell lines, using region-specific multiplex fluorescence in situ hybridization probes
dc.typeArticle
Author Affiliations
  1. The University of Hong Kong