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Article: Characterization of rearrangements involving 4q, 13q and 16q in hepatocellular carcinoma cell lines using region-specific multiplex-FISH probes

TitleCharacterization of rearrangements involving 4q, 13q and 16q in hepatocellular carcinoma cell lines using region-specific multiplex-FISH probes
Authors
Issue Date2007
PublisherElsevier Ireland Ltd. The Journal's web site is located at http://www.elsevier.com/locate/canlet
Citation
Cancer Letters, 2007, v. 250 n. 1, p. 92-99 How to Cite?
AbstractDeletions in 4q, 13q and 16q were frequently detected in hepatocellular carcinoma (HCC) by comparative genomic hybridization (CGH) studies. However, detailed chromosome structural aberrations are not fully explored. Using CGH combined with multiplex-color FISH (M-FISH) with chromosome region-specific probes (CRPs), chromosome structural aberrations in 4q, 13q and 16q in six HCC cell lines were studied. All CRPs, which were generated from microdissected DNA, were specific, strong in intensity and sensitive enough to detect chromosome structural aberrations including translocation and deletion. FISH with BAC probes was used to further characterize translocation breakpoints and deletions. A breakpoint at 16q22 was localized at a BAC clone (RP11-341K23) and another breakpoint at 4q28 was localized within a 620 kb-region. A minimal deleted region at 13q21 was found between BAC clones RP11-240M20 and RP11-435P18. This study demonstrated that the combination of CGH, M-FISH and BAC-FISH is a very useful tool to detect and characterize translocation breakpoint. © 2006 Elsevier Ireland Ltd. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/71922
ISSN
2015 Impact Factor: 5.992
2015 SCImago Journal Rankings: 2.331
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorTjia, WMen_HK
dc.contributor.authorHu, Len_HK
dc.contributor.authorZhang, MYen_HK
dc.contributor.authorGuan, XYen_HK
dc.date.accessioned2010-09-06T06:36:31Z-
dc.date.available2010-09-06T06:36:31Z-
dc.date.issued2007en_HK
dc.identifier.citationCancer Letters, 2007, v. 250 n. 1, p. 92-99en_HK
dc.identifier.issn0304-3835en_HK
dc.identifier.urihttp://hdl.handle.net/10722/71922-
dc.description.abstractDeletions in 4q, 13q and 16q were frequently detected in hepatocellular carcinoma (HCC) by comparative genomic hybridization (CGH) studies. However, detailed chromosome structural aberrations are not fully explored. Using CGH combined with multiplex-color FISH (M-FISH) with chromosome region-specific probes (CRPs), chromosome structural aberrations in 4q, 13q and 16q in six HCC cell lines were studied. All CRPs, which were generated from microdissected DNA, were specific, strong in intensity and sensitive enough to detect chromosome structural aberrations including translocation and deletion. FISH with BAC probes was used to further characterize translocation breakpoints and deletions. A breakpoint at 16q22 was localized at a BAC clone (RP11-341K23) and another breakpoint at 4q28 was localized within a 620 kb-region. A minimal deleted region at 13q21 was found between BAC clones RP11-240M20 and RP11-435P18. This study demonstrated that the combination of CGH, M-FISH and BAC-FISH is a very useful tool to detect and characterize translocation breakpoint. © 2006 Elsevier Ireland Ltd. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherElsevier Ireland Ltd. The Journal's web site is located at http://www.elsevier.com/locate/canleten_HK
dc.relation.ispartofCancer Lettersen_HK
dc.rightsCancer Letters. Copyright © Elsevier Ireland Ltd.en_HK
dc.subject.meshCarcinoma, Hepatocellular - geneticsen_HK
dc.subject.meshCell Line, Tumoren_HK
dc.subject.meshChromosome Aberrationsen_HK
dc.subject.meshChromosomes, Human, Pair 13en_HK
dc.subject.meshChromosomes, Human, Pair 16en_HK
dc.subject.meshChromosomes, Human, Pair 4en_HK
dc.subject.meshHumansen_HK
dc.subject.meshIn Situ Hybridization, Fluorescence - methodsen_HK
dc.subject.meshLiver Neoplasms - geneticsen_HK
dc.subject.meshTranslocation, Geneticen_HK
dc.titleCharacterization of rearrangements involving 4q, 13q and 16q in hepatocellular carcinoma cell lines using region-specific multiplex-FISH probesen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0304-3835&volume=250&spage=92&epage=99&date=2007&atitle=Characterization+of+rearrangements+involving+4q,+13q+and+16q+in+hepatocellular+carcinoma+cell+lines+using+region-specific+multiplex-FISH+probes.en_HK
dc.identifier.emailGuan, XY:xyguan@hkucc.hku.hken_HK
dc.identifier.authorityGuan, XY=rp00454en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.canlet.2006.09.023en_HK
dc.identifier.pmid17098359-
dc.identifier.scopuseid_2-s2.0-33947582844en_HK
dc.identifier.hkuros133761en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-33947582844&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume250en_HK
dc.identifier.issue1en_HK
dc.identifier.spage92en_HK
dc.identifier.epage99en_HK
dc.identifier.isiWOS:000246260100011-
dc.publisher.placeIrelanden_HK
dc.identifier.scopusauthoridTjia, WM=8631854600en_HK
dc.identifier.scopusauthoridHu, L=34770075600en_HK
dc.identifier.scopusauthoridZhang, MY=8722491600en_HK
dc.identifier.scopusauthoridGuan, XY=7201463221en_HK

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