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Article: Detection of wild type and deleted latent membrane protein 1 (LMP1) of Epstein-Barr virus in clinical biopsy material

TitleDetection of wild type and deleted latent membrane protein 1 (LMP1) of Epstein-Barr virus in clinical biopsy material
Authors
KeywordsEpstein-Barr virus (EBV)
Hodgkin's disease
Immunohistochemistry
Latent membrane protein (LMP 1)
Monoclonal antibodies
Nasopharyngeal carcinoma
Issue Date2004
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jviromet
Citation
Journal Of Virological Methods, 2004, v. 116 n. 1, p. 79-88 How to Cite?
AbstractThe latent membrane protein 1 (LMP1) of Epstein-Barr virus (EBV) is one of two postulated viral oncogenic proteins. Sequence variations, and in particular a 30 base pair deletion variant called CAO, may define different disease populations. We developed a panel of rat monoclonal antibodies (MAb) specific for the non-wild type LMP1 and compared the presence of the antibody staining with LMP1 DNA sequence analysis on clinical samples of nasopharyngeal carcinoma, peripheral T-cell lymphoma (PTCL), Hodgkin's disease, lymphoblastoid cell lines (LCL) from normal volunteers, and patients with nasopharyngeal carcinoma. The results demonstrate specificity of the monoclonal cocktail for detecting the non-wild type LMP1 and the ability to sub-differentiate between the mediterranean type of LMP1 and the CAO-LMP1. Double immunofluorescence on paraffin material using the traditional CS1-4 monoclonal antibodies and the CAO-cocktail revealed no dual population of cells in the biopsy material from the Asian region. © 2003 Elsevier B.V. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/71921
ISSN
2015 Impact Factor: 1.508
2015 SCImago Journal Rankings: 0.866
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorNicholls, Jen_HK
dc.contributor.authorHahn, Pen_HK
dc.contributor.authorKremmer, Een_HK
dc.contributor.authorFröhlich, Ten_HK
dc.contributor.authorArnold, GJen_HK
dc.contributor.authorSham, Jen_HK
dc.contributor.authorKwong, Den_HK
dc.contributor.authorGrässer, FAen_HK
dc.date.accessioned2010-09-06T06:36:31Z-
dc.date.available2010-09-06T06:36:31Z-
dc.date.issued2004en_HK
dc.identifier.citationJournal Of Virological Methods, 2004, v. 116 n. 1, p. 79-88en_HK
dc.identifier.issn0166-0934en_HK
dc.identifier.urihttp://hdl.handle.net/10722/71921-
dc.description.abstractThe latent membrane protein 1 (LMP1) of Epstein-Barr virus (EBV) is one of two postulated viral oncogenic proteins. Sequence variations, and in particular a 30 base pair deletion variant called CAO, may define different disease populations. We developed a panel of rat monoclonal antibodies (MAb) specific for the non-wild type LMP1 and compared the presence of the antibody staining with LMP1 DNA sequence analysis on clinical samples of nasopharyngeal carcinoma, peripheral T-cell lymphoma (PTCL), Hodgkin's disease, lymphoblastoid cell lines (LCL) from normal volunteers, and patients with nasopharyngeal carcinoma. The results demonstrate specificity of the monoclonal cocktail for detecting the non-wild type LMP1 and the ability to sub-differentiate between the mediterranean type of LMP1 and the CAO-LMP1. Double immunofluorescence on paraffin material using the traditional CS1-4 monoclonal antibodies and the CAO-cocktail revealed no dual population of cells in the biopsy material from the Asian region. © 2003 Elsevier B.V. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jvirometen_HK
dc.relation.ispartofJournal of Virological Methodsen_HK
dc.rightsJournal of Virological Methods. Copyright © Elsevier BV.en_HK
dc.subjectEpstein-Barr virus (EBV)en_HK
dc.subjectHodgkin's diseaseen_HK
dc.subjectImmunohistochemistryen_HK
dc.subjectLatent membrane protein (LMP 1)en_HK
dc.subjectMonoclonal antibodiesen_HK
dc.subjectNasopharyngeal carcinomaen_HK
dc.subject.meshAmino Acid Sequenceen_HK
dc.subject.meshAntibodies, Monoclonal - immunologyen_HK
dc.subject.meshAntibodies, Viral - immunologyen_HK
dc.subject.meshAntibody Specificityen_HK
dc.subject.meshAntigens, Viral - immunologyen_HK
dc.subject.meshBiopsyen_HK
dc.subject.meshCell Line, Transformeden_HK
dc.subject.meshCell Line, Tumoren_HK
dc.subject.meshDNA, Viral - analysis - isolation & purificationen_HK
dc.subject.meshEpstein-Barr Virus Infections - virologyen_HK
dc.subject.meshGenes, Viralen_HK
dc.subject.meshHodgkin Disease - virologyen_HK
dc.subject.meshHumansen_HK
dc.subject.meshImmunohistochemistryen_HK
dc.subject.meshLymphocytes - virologyen_HK
dc.subject.meshLymphoma, T-Cell, Peripheral - virologyen_HK
dc.subject.meshMolecular Sequence Dataen_HK
dc.subject.meshNasopharyngeal Neoplasms - virologyen_HK
dc.subject.meshNucleic Acid Amplification Techniquesen_HK
dc.subject.meshOncogene Proteins, Viral - analysis - chemistry - genetics - immunologyen_HK
dc.subject.meshSequence Deletionen_HK
dc.subject.meshViral Matrix Proteins - analysis - chemistry - genetics - immunologyen_HK
dc.titleDetection of wild type and deleted latent membrane protein 1 (LMP1) of Epstein-Barr virus in clinical biopsy materialen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0166-0934&volume=116&issue=1&spage=79&epage=88 &date=2004&atitle=Detection+of+wild+type+and+deleted+latent+membrane+protein+1+(LMP1)+of+Epstein-Barr+virus+in+clinical+biopsy+materialen_HK
dc.identifier.emailNicholls, J:nicholls@pathology.hku.hken_HK
dc.identifier.emailKwong, D:dlwkwong@hku.hken_HK
dc.identifier.authorityNicholls, J=rp00364en_HK
dc.identifier.authorityKwong, D=rp00414en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.jviromet.2003.10.015en_HK
dc.identifier.pmid14715310-
dc.identifier.scopuseid_2-s2.0-0346727188en_HK
dc.identifier.hkuros86069en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0346727188&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume116en_HK
dc.identifier.issue1en_HK
dc.identifier.spage79en_HK
dc.identifier.epage88en_HK
dc.identifier.isiWOS:000188380600010-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridNicholls, J=7201463077en_HK
dc.identifier.scopusauthoridHahn, P=7202817936en_HK
dc.identifier.scopusauthoridKremmer, E=24179132500en_HK
dc.identifier.scopusauthoridFröhlich, T=8277190700en_HK
dc.identifier.scopusauthoridArnold, GJ=35301536600en_HK
dc.identifier.scopusauthoridSham, J=24472255400en_HK
dc.identifier.scopusauthoridKwong, D=15744231600en_HK
dc.identifier.scopusauthoridGrässer, FA=7004302929en_HK

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