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Article: Surface-enhanced laser desorption/ionization time-of-flight mass spectrometry serum protein profiling to identify nasopharyngeal carcinoma

TitleSurface-enhanced laser desorption/ionization time-of-flight mass spectrometry serum protein profiling to identify nasopharyngeal carcinoma
Authors
KeywordsBiomarker
Classification and regression tree (CART)
Mass spectrometry
Nasopharyngeal carcinoma (NPC)
Surface-enhanced laser desorption/ionization (SELDI)
Issue Date2006
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/28741
Citation
Cancer, 2006, v. 107 n. 1, p. 99-107 How to Cite?
AbstractBACKGROUND. Diagnosis of nasopharyngeal carcinoma (NPC) at an early disease-stage is important for successful treatment and improving the outcome of patients. The use of serum protein profiles and a classification tree algorithm were explored to distinguish NPC from noncancer. METHODS. Serum samples were applied to metal affinity protein chips to generate mass spectra by surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF-MS). Protein peak identification and clustering were performed using the Biomarker Wizard software. Proteomic spectra of serum samples from 50 NPC patients and 54 noncancer controls were used as a training set and a classification tree with 6 distinct protein masses was generated by using Biomarker Pattern software. The validity of the classification tree was then challenged with a blind test set including another 20 NPC patients and 25 noncancer controls. RESULTS. The software identified an average of 93 mass peaks/spectrum and 6 of the identified peaks were used to construct the classification tree. The classification tree correctly determined 83% (123 of 149) of the test samples with 83% (58 of 70) of the NPC samples and 82% (65 of 79) of the noncancer samples. In a combination of the serum protein profiles with Epstein-Barr (EBV) nuclear antigen 1 (EBNAI IgA) test, the diagnostic sensitivity and specificity were increased to 99% and 96%, respectively. CONCLUSIONS. The results suggest that SELDI-TOF-MS serum protein profiles could discriminate NPC from noncancer. The combination of serum protein profiles with an EBV antibody serology test could further improve the accuracy of NPC screening. © 2006 American Cancer Society.
Persistent Identifierhttp://hdl.handle.net/10722/71904
ISSN
2014 Impact Factor: 4.889
2014 SCImago Journal Rankings: 2.510
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorHo, DWYen_HK
dc.contributor.authorYang, ZFen_HK
dc.contributor.authorWong, BYHen_HK
dc.contributor.authorKwong, DLWen_HK
dc.contributor.authorSham, JSTen_HK
dc.contributor.authorWei, WIen_HK
dc.contributor.authorYuen, APWen_HK
dc.date.accessioned2010-09-06T06:36:20Z-
dc.date.available2010-09-06T06:36:20Z-
dc.date.issued2006en_HK
dc.identifier.citationCancer, 2006, v. 107 n. 1, p. 99-107en_HK
dc.identifier.issn0008-543Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/71904-
dc.description.abstractBACKGROUND. Diagnosis of nasopharyngeal carcinoma (NPC) at an early disease-stage is important for successful treatment and improving the outcome of patients. The use of serum protein profiles and a classification tree algorithm were explored to distinguish NPC from noncancer. METHODS. Serum samples were applied to metal affinity protein chips to generate mass spectra by surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF-MS). Protein peak identification and clustering were performed using the Biomarker Wizard software. Proteomic spectra of serum samples from 50 NPC patients and 54 noncancer controls were used as a training set and a classification tree with 6 distinct protein masses was generated by using Biomarker Pattern software. The validity of the classification tree was then challenged with a blind test set including another 20 NPC patients and 25 noncancer controls. RESULTS. The software identified an average of 93 mass peaks/spectrum and 6 of the identified peaks were used to construct the classification tree. The classification tree correctly determined 83% (123 of 149) of the test samples with 83% (58 of 70) of the NPC samples and 82% (65 of 79) of the noncancer samples. In a combination of the serum protein profiles with Epstein-Barr (EBV) nuclear antigen 1 (EBNAI IgA) test, the diagnostic sensitivity and specificity were increased to 99% and 96%, respectively. CONCLUSIONS. The results suggest that SELDI-TOF-MS serum protein profiles could discriminate NPC from noncancer. The combination of serum protein profiles with an EBV antibody serology test could further improve the accuracy of NPC screening. © 2006 American Cancer Society.en_HK
dc.languageengen_HK
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/28741en_HK
dc.relation.ispartofCanceren_HK
dc.rightsCancer. Copyright © John Wiley & Sons, Inc.en_HK
dc.subjectBiomarkeren_HK
dc.subjectClassification and regression tree (CART)en_HK
dc.subjectMass spectrometryen_HK
dc.subjectNasopharyngeal carcinoma (NPC)en_HK
dc.subjectSurface-enhanced laser desorption/ionization (SELDI)en_HK
dc.subject.meshAdulten_HK
dc.subject.meshAgeden_HK
dc.subject.meshAged, 80 and overen_HK
dc.subject.meshAlgorithmsen_HK
dc.subject.meshBiological Markers - blooden_HK
dc.subject.meshDecision Treesen_HK
dc.subject.meshFemaleen_HK
dc.subject.meshHumansen_HK
dc.subject.meshMaleen_HK
dc.subject.meshMicrochip Analytical Proceduresen_HK
dc.subject.meshMiddle Ageden_HK
dc.subject.meshNasopharyngeal Neoplasms - blood - classification - diagnosisen_HK
dc.subject.meshNeoplasm Proteins - blooden_HK
dc.subject.meshReproducibility of Resultsen_HK
dc.subject.meshSensitivity and Specificityen_HK
dc.subject.meshSpectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methodsen_HK
dc.subject.meshSurface Propertiesen_HK
dc.titleSurface-enhanced laser desorption/ionization time-of-flight mass spectrometry serum protein profiling to identify nasopharyngeal carcinomaen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0008-543X&volume=107&issue=1&spage=99&epage=107&date=2006&atitle=Surface-enhanced+laser+desorption/ionization+time-of-flight+mass+spectrometry+serum+protein+profiling+to+identify+nasopharyngeal+carcinomaen_HK
dc.identifier.emailKwong, DLW: dlwkwong@hku.hken_HK
dc.identifier.emailWei, WI: hrmswwi@hku.hken_HK
dc.identifier.authorityKwong, DLW=rp00414en_HK
dc.identifier.authorityWei, WI=rp00323en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1002/cncr.21970en_HK
dc.identifier.pmid16708360en_HK
dc.identifier.scopuseid_2-s2.0-33745318656en_HK
dc.identifier.hkuros119065en_HK
dc.identifier.hkuros137546-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-33745318656&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume107en_HK
dc.identifier.issue1en_HK
dc.identifier.spage99en_HK
dc.identifier.epage107en_HK
dc.identifier.isiWOS:000238469500013-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridHo, DWY=7402971906en_HK
dc.identifier.scopusauthoridYang, ZF=14018809600en_HK
dc.identifier.scopusauthoridWong, BYH=7402023295en_HK
dc.identifier.scopusauthoridKwong, DLW=15744231600en_HK
dc.identifier.scopusauthoridSham, JST=24472255400en_HK
dc.identifier.scopusauthoridWei, WI=7403321552en_HK
dc.identifier.scopusauthoridYuen, APW=7006290111en_HK

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