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Article: Application of ultrafiltration to improve the extraction of antibiotics

TitleApplication of ultrafiltration to improve the extraction of antibiotics
Authors
KeywordsAntibiotics
Emulsion
Solvent extraction
Ultrafiltration
Issue Date2004
PublisherPergamon. The Journal's web site is located at http://www.elsevier.com/locate/seppur
Citation
Separation And Purification Technology, 2004, v. 34 n. 1-3, p. 115-123 How to Cite?
AbstractThe production of antibiotics typically consists of fermentation, removal of biomass, solvent extraction, and crystallization. Vacuum rotary filtration or other conventional filtration systems and microfiltration are used to remove the biomass, but the biomass removal step fails to remove macromolecules including polysaccharides and proteins which may act as emulsifiers causing emulsion in the subsequent solvent extraction process, and reducing the process efficiency and product quality. In this work, an attempt was made to remove these "emulsifiers" before solvent extraction using ultrafiltration (UF). Bench and pilot scale spiral wound membrane modules with the MWCO of 5, 20 and 50 kDa were tested to treat benzylpenicillin (Pen G) broth following microbe removal. The UF permeates were subsequently fed to solvent extraction (acidic extraction at pH 2.0). The experimental results showed that UF could significantly improve the extraction operation in terms of phase separation, elimination of the need for any de-emulsifier or wet agent, and increase extraction recovery and product quality. Similar improvements from UF were observed in the extractions of erythromycin and medmycin (alkaline extraction at pH 10.0 and 8.7, respectively). The experimental results show that UF is an alternative to the use of de-emulsifier or other wet agent to obtain good phase separation even by gravity without centrifugal extractor in solvent extractions of Pen G, erythromycin and medmycin. The mechanisms of the improvement of extraction were discussed based on the experimental results on characterizing the feed and permeate solutions of UF. And it was concluded that there existed bioemulsifiers in antibiotic fermentation broth and UF could successfully remove them and hence could improve the extraction operation. © 2003 Published by Elsevier B.V.
Persistent Identifierhttp://hdl.handle.net/10722/71706
ISSN
2015 Impact Factor: 3.299
2015 SCImago Journal Rankings: 1.100
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLi, SZen_HK
dc.contributor.authorLi, XYen_HK
dc.contributor.authorCui, ZFen_HK
dc.contributor.authorWang, DZen_HK
dc.date.accessioned2010-09-06T06:34:26Z-
dc.date.available2010-09-06T06:34:26Z-
dc.date.issued2004en_HK
dc.identifier.citationSeparation And Purification Technology, 2004, v. 34 n. 1-3, p. 115-123en_HK
dc.identifier.issn1383-5866en_HK
dc.identifier.urihttp://hdl.handle.net/10722/71706-
dc.description.abstractThe production of antibiotics typically consists of fermentation, removal of biomass, solvent extraction, and crystallization. Vacuum rotary filtration or other conventional filtration systems and microfiltration are used to remove the biomass, but the biomass removal step fails to remove macromolecules including polysaccharides and proteins which may act as emulsifiers causing emulsion in the subsequent solvent extraction process, and reducing the process efficiency and product quality. In this work, an attempt was made to remove these "emulsifiers" before solvent extraction using ultrafiltration (UF). Bench and pilot scale spiral wound membrane modules with the MWCO of 5, 20 and 50 kDa were tested to treat benzylpenicillin (Pen G) broth following microbe removal. The UF permeates were subsequently fed to solvent extraction (acidic extraction at pH 2.0). The experimental results showed that UF could significantly improve the extraction operation in terms of phase separation, elimination of the need for any de-emulsifier or wet agent, and increase extraction recovery and product quality. Similar improvements from UF were observed in the extractions of erythromycin and medmycin (alkaline extraction at pH 10.0 and 8.7, respectively). The experimental results show that UF is an alternative to the use of de-emulsifier or other wet agent to obtain good phase separation even by gravity without centrifugal extractor in solvent extractions of Pen G, erythromycin and medmycin. The mechanisms of the improvement of extraction were discussed based on the experimental results on characterizing the feed and permeate solutions of UF. And it was concluded that there existed bioemulsifiers in antibiotic fermentation broth and UF could successfully remove them and hence could improve the extraction operation. © 2003 Published by Elsevier B.V.en_HK
dc.languageengen_HK
dc.publisherPergamon. The Journal's web site is located at http://www.elsevier.com/locate/seppuren_HK
dc.relation.ispartofSeparation and Purification Technologyen_HK
dc.subjectAntibioticsen_HK
dc.subjectEmulsionen_HK
dc.subjectSolvent extractionen_HK
dc.subjectUltrafiltrationen_HK
dc.titleApplication of ultrafiltration to improve the extraction of antibioticsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1383-5866&volume=34&spage=115&epage=123&date=2004&atitle=Application+of+ultrafiltration+to+improve+the+extraction+of+antibioticsen_HK
dc.identifier.emailLi, XY:xlia@hkucc.hku.hken_HK
dc.identifier.authorityLi, XY=rp00222en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/S1383-5866(03)00185-0en_HK
dc.identifier.scopuseid_2-s2.0-0942280496en_HK
dc.identifier.hkuros90830en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0942280496&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume34en_HK
dc.identifier.issue1-3en_HK
dc.identifier.spage115en_HK
dc.identifier.epage123en_HK
dc.identifier.isiWOS:000188537500017-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridLi, SZ=26660776400en_HK
dc.identifier.scopusauthoridLi, XY=26642887900en_HK
dc.identifier.scopusauthoridCui, ZF=7202504467en_HK
dc.identifier.scopusauthoridWang, DZ=7407068226en_HK

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