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Article: Characterization of electrostatic binding sites of extracellular polymers by linear programming analysis of titration data

TitleCharacterization of electrostatic binding sites of extracellular polymers by linear programming analysis of titration data
Authors
Issue Date2002
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/71002188
Citation
Biotechnology And Bioengineering, 2002, v. 80 n. 7, p. 806-811 How to Cite?
AbstractElectrostatic binding sites of extracellular polymeric substances (EPS) were characterized from titration data using linear programming analysis. Test results for three synthetic solutions of given solutes comprising amino, carboxyl, and phenolic groups indicated that this method was able to identify the electrostatic binding sites. For the six sites with pKa between 3 and 10, the estimated pKa deviated 0.11 ± 0.09 from the theoretical values, and the estimated concentrations deviated 3.0% ± 0.9% from the actual concentrations. Two EPS samples were then extracted from a hydrogen-producing sludge (HPS) and a sulfate-reducing biofilm (SRB). Analysis of charge excess data in titration from pH 3 to 11 indicated that the EPS of HPS comprised of five electrostatic binding sites with pKa ranging from 3 to 11. The pKa values of these binding sites and the possible corresponding functional groups were pKa 4.8 (carboxyl), pKa 6.0 (carboxyl/phosphoric), pKa 7.0 (phosphoric), pKa 9.8 (amine/phenolic), and pKa 11.0 (hydroxyl). EPS of the SRB comprised five of similar binding sites (with corresponding pKa values of 4.4, 6.0, 7.4, 9.4, and 11.0), plus one extra site at pKa 8.2, which was likely corresponding to the sulfhydryl group. The total electrostatic binding site concentration of EPS extracted from HPS were 10.88 mmol/g-EPS, of which the highest concentration was from the site of pKa 11.0. The corresponding values for the EPS extracted from SRB were 16.44 mmol/g-EPS and pKa 4.4. The total concentrations of electrostatic binding sites found in this study were 20- to 30-fold of those reported for bacterial cell surface, implying that EPS might be more crucial in biosorption of metals than bacterial cell surface in wastewater treatment and in bioremediation. © 2002 Wiley Periodicals, Inc.
Persistent Identifierhttp://hdl.handle.net/10722/71471
ISSN
2015 Impact Factor: 4.243
2015 SCImago Journal Rankings: 1.633
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLiu, Hen_HK
dc.contributor.authorFang, HHPen_HK
dc.date.accessioned2010-09-06T06:32:18Z-
dc.date.available2010-09-06T06:32:18Z-
dc.date.issued2002en_HK
dc.identifier.citationBiotechnology And Bioengineering, 2002, v. 80 n. 7, p. 806-811en_HK
dc.identifier.issn0006-3592en_HK
dc.identifier.urihttp://hdl.handle.net/10722/71471-
dc.description.abstractElectrostatic binding sites of extracellular polymeric substances (EPS) were characterized from titration data using linear programming analysis. Test results for three synthetic solutions of given solutes comprising amino, carboxyl, and phenolic groups indicated that this method was able to identify the electrostatic binding sites. For the six sites with pKa between 3 and 10, the estimated pKa deviated 0.11 ± 0.09 from the theoretical values, and the estimated concentrations deviated 3.0% ± 0.9% from the actual concentrations. Two EPS samples were then extracted from a hydrogen-producing sludge (HPS) and a sulfate-reducing biofilm (SRB). Analysis of charge excess data in titration from pH 3 to 11 indicated that the EPS of HPS comprised of five electrostatic binding sites with pKa ranging from 3 to 11. The pKa values of these binding sites and the possible corresponding functional groups were pKa 4.8 (carboxyl), pKa 6.0 (carboxyl/phosphoric), pKa 7.0 (phosphoric), pKa 9.8 (amine/phenolic), and pKa 11.0 (hydroxyl). EPS of the SRB comprised five of similar binding sites (with corresponding pKa values of 4.4, 6.0, 7.4, 9.4, and 11.0), plus one extra site at pKa 8.2, which was likely corresponding to the sulfhydryl group. The total electrostatic binding site concentration of EPS extracted from HPS were 10.88 mmol/g-EPS, of which the highest concentration was from the site of pKa 11.0. The corresponding values for the EPS extracted from SRB were 16.44 mmol/g-EPS and pKa 4.4. The total concentrations of electrostatic binding sites found in this study were 20- to 30-fold of those reported for bacterial cell surface, implying that EPS might be more crucial in biosorption of metals than bacterial cell surface in wastewater treatment and in bioremediation. © 2002 Wiley Periodicals, Inc.en_HK
dc.languageengen_HK
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/71002188en_HK
dc.relation.ispartofBiotechnology and Bioengineeringen_HK
dc.rightsBiotechnology and Bioengineering. Copyright © John Wiley & Sons, Inc.en_HK
dc.subject.meshBacteria - chemistry - classification - metabolismen_HK
dc.subject.meshBinding Sitesen_HK
dc.subject.meshBiofilmsen_HK
dc.subject.meshCell Extracts - chemistryen_HK
dc.subject.meshCell Wall - chemistry - metabolismen_HK
dc.subject.meshClostridium - chemistry - metabolismen_HK
dc.subject.meshComputer Simulationen_HK
dc.subject.meshConductometry - methodsen_HK
dc.subject.meshElectrochemistry - methodsen_HK
dc.subject.meshHydrogen-Ion Concentrationen_HK
dc.subject.meshLactobacillaceae - chemistry - metabolismen_HK
dc.subject.meshModels, Chemicalen_HK
dc.subject.meshPolymers - chemistry - metabolismen_HK
dc.subject.meshProgramming, Linearen_HK
dc.subject.meshSewage - chemistry - microbiologyen_HK
dc.subject.meshSolutions - chemistryen_HK
dc.subject.meshSpecies Specificityen_HK
dc.subject.meshStatic Electricityen_HK
dc.subject.meshSulfur-Reducing Bacteria - chemistry - metabolismen_HK
dc.titleCharacterization of electrostatic binding sites of extracellular polymers by linear programming analysis of titration dataen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0006-3592&volume=80&issue=7&spage=806&epage=811&date=2002&atitle=Characterization+of+electrostatic+binding+sites+of+extracellular+polymers+by+linear+programming+analysis+of+titration+dataen_HK
dc.identifier.emailFang, HHP:hrechef@hkucc.hku.hken_HK
dc.identifier.authorityFang, HHP=rp00115en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1002/bit.10432en_HK
dc.identifier.pmid12402326-
dc.identifier.scopuseid_2-s2.0-0037203060en_HK
dc.identifier.hkuros76005en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0037203060&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume80en_HK
dc.identifier.issue7en_HK
dc.identifier.spage806en_HK
dc.identifier.epage811en_HK
dc.identifier.isiWOS:000179426000009-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridLiu, H=36072584800en_HK
dc.identifier.scopusauthoridFang, HHP=7402542625en_HK

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